##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII AccIII AclI AflII Aor51HI
AscI AseI AsnI BbsI BclI
BfrI BlpI Bpu1102I BpuAI BsaBI
BseAI BsiWI BspDI BspEI BssHII
Bst1107I BstZ17I CelII ClaI CpoI
DrdI Eco47III EcoRV EcoT22I FbaI
FseI HindIII HpaI KspI MamI
MfeI MroI MunI NdeI NotI
NruI NsiI PacI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SbfI ScaI SfiI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 4313 GGTACC G^GTACC
AgeI 4316 ACCGGT A^CCGGT
Asp718I 4313 GGTACC G^GTACC
BbrPI 4239 CACGTG CAC^GTG
BcgI 3466 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 617 GTATCC GTATCC(6/5)
BglII 210 AGATCT A^GATCT
BsaAI 4239 CACGTG [CT]AC^GT[GA]
BsaWI 4316 ACCGGT [AT]^CCGG[AT]
BsiEI 975 CGGCCG CG[GA][CT]^CG
BspLU11I 1845 ACATGT A^CATGT
BsrDI 5205 CATTGC GCAATG(2/0)
BstBI 4085 TTCGAA TT^CGAA
DraI 2608 TTTAAA TTT^AAA
EagI 975 CGGCCG C^GGCCG
EclXI 975 CGGCCG C^GGCCG
Eco52I 975 CGGCCG C^GGCCG
EcoRI 5131 GAATTC G^AATTC
KpnI 4313 GGTACC GGTAC^C
MluI 1863 ACGCGT A^CGCGT
NaeI 324 GCCGGC GCC^GGC
NcoI 3760 CCATGG C^CATGG
NgoMIV 324 GCCGGC G^CCGGC
NheI 3861 GCTAGC G^CTAGC
NspV 4085 TTCGAA TT^CGAA
PaeR7I 2285 CTCGAG C^TCGAG
PflMI 1726 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 4316 ACCGGT A^CCGGT
PmaCI 4239 CACGTG CAC^GTG
PmlI 4239 CACGTG CAC^GTG
SalI 341 GTCGAC G^TCGAC
SapI 808 GCTCTTC GCTCTTC(1/4)
SfuI 4085 TTCGAA TT^CGAA
Van91I 1726 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2127 TCTAGA T^CTAGA
XhoI 2285 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 1845 ACATGT A^C[GA][CT]GT
1863 ACGCGT
Alw44I 242 GTGCAC G^TGCAC
1859 GTGCAC
ApaLI 242 GTGCAC G^TGCAC
1859 GTGCAC
Asp700I 1357 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4485 GAATGGCTTC
AspI 935 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
5567 GACCCTGTC
AviII 741 TGCGCA TGC^GCA
783 TGCGCA
AvrII 4074 CCTAGG C^CTAGG
5068 CCTAGG
BamHI 4105 GGATCC G^GATCC
4644 GGATCC
BlnI 4074 CCTAGG C^CTAGG
5068 CCTAGG
BsmI 3346 GAATGC GAATGC(1/-1)
4443 GCATTC
Bsp1407I 864 TGTACA T^GTACA
5413 TGTACA
BsrGI 864 TGTACA T^GTACA
5413 TGTACA
BstEII 115 GGTCACC G^GT[ACGT]ACC
1536 GGTCACC
BstPI 115 GGTCACC G^GT[ACGT]ACC
1536 GGTCACC
EcoO65I 115 GGTCACC G^GT[ACGT]ACC
1536 GGTCACC
FspI 741 TGCGCA TGC^GCA
783 TGCGCA
HincII 341 GTCGAC GT[CT]^[GA]AC
4666 GTTGAC
HindII 341 GTCGAC GT[CT]^[GA]AC
4666 GTTGAC
NspI 1845 ACATGT [GA]CATG^[CT]
1877 GCATGT
PflFI 935 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
5567 GACCCTGTC
SspBI 864 TGTACA T^GTACA
5413 TGTACA
StuI 1571 AGGCCT AGG^CCT
3748 AGGCCT
Tth111I 935 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
5567 GACCCTGTC
XcmI 2922 CCAGGGAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5204 CCATTGCTTCACTGG
XmnI 1357 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4485 GAATGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AhdI AluI AlwI AlwNI
AocI ApaI ApoI AspEI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspHI BspMI BsrBI BsrFI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
Eam1105I EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII RcaI RsaI
SacI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SfoI SmaI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmaCI XmaI