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| Product ID | ORK01973 | 
|---|---|
| Accession No | AB007860 | 
| Description | ArfGAP with SH3 domain, ankyrin repeat and PH domain 2, transcript variant 1 | 
| Clone name | hg01091 | 
| Vector information | |
| cDNA sequence | DNA sequence (5711 bp) Predicted protein sequence (1022 aa) | 
| 
    HaloTag ORF Clone | 
    FHC01973
       | 
| Flexi ORF Clone | FXC01973 | 
| Source | Human adult brain | 
| Rouge ID | mKIAA0400
    
    by Kazusa Mouse cDNA Project | 
 Length: 5711 bp
 Length: 5711 bp Physical map
 Physical map 
     Restriction map
 Restriction map Prediction of protein coding region (GeneMark analysis).
 Prediction of protein coding region (GeneMark analysis).
    | N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | No warning | No warning | 
 Length: 1022 aa
 
        Length: 1022 aa Result of homology search against nr database 
        (FASTA output,
	Multiple alignment)
        Result of homology search against nr database 
        (FASTA output,
	Multiple alignment)|  | 
 | 
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
 Result of homology search against HUGE database
        (FASTA output,
	Multiple alignment)
        Result of homology search against HUGE database
        (FASTA output,
	Multiple alignment)|  | 
 | 
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
 Result of motif / domain search (InterProScan and SOSUI)
	Result of motif / domain search (InterProScan and SOSUI) Result of InterProScan
 Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition | 
|---|---|---|---|---|---|
| BlastProDom | IPR001452 | 963 | 1017 | PD000066 | Src homology-3 | 
| FPrintScan | IPR001164 | 449 | 468 | PR00405 | Arf GTPase activating protein | 
| IPR001164 | 468 | 485 | PR00405 | Arf GTPase activating protein | |
| IPR001164 | 489 | 510 | PR00405 | Arf GTPase activating protein | |
| IPR001452 | 977 | 992 | PR00452 | Src homology-3 | |
| IPR001452 | 994 | 1003 | PR00452 | Src homology-3 | |
| IPR001452 | 1008 | 1020 | PR00452 | Src homology-3 | |
| HMMPfam | IPR001849 | 322 | 413 | PF00169 | Pleckstrin-like | 
| IPR001164 | 437 | 559 | PF01412 | Arf GTPase activating protein | |
| IPR002110 | 600 | 635 | PF00023 | Ankyrin | |
| IPR002110 | 636 | 668 | PF00023 | Ankyrin | |
| IPR002110 | 669 | 689 | PF00023 | Ankyrin | |
| IPR001452 | 963 | 1017 | PF00018 | Src homology-3 | |
| HMMSmart | IPR001849 | 322 | 415 | SM00233 | Pleckstrin-like | 
| IPR001164 | 437 | 557 | SM00105 | Arf GTPase activating protein | |
| IPR002110 | 600 | 632 | SM00248 | Ankyrin | |
| IPR002110 | 636 | 665 | SM00248 | Ankyrin | |
| IPR002110 | 669 | 699 | SM00248 | Ankyrin | |
| IPR001452 | 963 | 1021 | SM00326 | Src homology-3 | |
| ProfileScan | IPR001849 | 321 | 413 | PS50003 | Pleckstrin-like | 
| IPR001164 | 437 | 559 | PS50115 | Arf GTPase activating protein | |
| IPR002110 | 600 | 691 | PS50297 | Ankyrin | |
| IPR002110 | 636 | 668 | PS50088 | Ankyrin | |
| IPR001452 | 960 | 1022 | PS50002 | Src homology-3 | 
|  RT-PCR | 
|---|
 Experimental conditions
 Experimental conditions| Primer_f | GCTGCAACCAAAGTAATCAGG | 
|---|---|
| Primer_r | GTGTGATCTGTTAAGGGGTGG | 
| PCR conditions | 95 °C  30 sec  55 °C  30 sec  72 °C  60 sec  30 cycles  | 
 Chromosome No. 2
 Chromosome No. 2 Experimental conditions
 Experimental conditions| Panel name | GeneBridge 4 | 
|---|---|
| Primer_f | GCTGCAACCAAAGTAATCAGG | 
| Primer_r | GTGTGATCTGTTAAGGGGTGG | 
| PCR product length | 100 bp | 
| PCR conditions | 95 °C  15 sec  64 °C  60 sec  30 cycles | 
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