Gene/Protein Characteristic Table for KIAA0732
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Product ID ORK00120
Accession No AB018275
Description SMG6 nonsense mediated mRNA decay factor, transcript variant 1
Clone name fg05988b
Vector information
The cDNA fragment was inserted at the NotI site of the
cDNA sequence DNA sequence (6002 bp)
Predicted protein sequence (1449 aa)
Flexi ORF Clone FXC00120
Source Human fetal brain
Rouge ID mKIAA0732 by Kazusa Mouse cDNA Project
Note We replaced hk03717, former representative clones for KIAA0732 with fg05988b. (2001/5/29)
Features of the cloned cDNA sequence
Description

Length: 6002 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning Warning
Integrity of 3' end
Length of 3'UTR 1650 bp
Genome contig ID gi51511734r_1809888
PolyA signal sequence
(AATAAA,-24)
+----*----+----*----+----*----+----
TTTGTTTCCTTAATAAATTTTTAGTTATGAAACAT
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
ACCTGACCTCTTGCTGATCTGTTTCTGGGACTGGGGAGGGAGGCAGGTCC

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 17 r 1909888 2153826 19 99.4 Terminal No-hit
Features of the protein sequence
Description

Length: 1449 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
EAW90558 0 99.9 hCG1813980, iso...
Homo sapiens
BAG11233 0 100.0 Smg-6 homolog, ...
synthetic construct
AAN46114 0 99.9 telomerase subu...
Homo sapiens
Q86US8 0 99.9 Telomerase-bind...
Homo sapiens
AAO17581 0 99.9 Est1p-like prot...
Homo sapiens
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
D87437 2.1e-08 24.1 KIAA0250
AB029012 7.2e-05 24.8 KIAA1089
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMSmart IPR006596 1276 1427 SM00670 Nucleotide binding protein
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f TCTTCCCCTTCTAGTTGATCC
Primer_r CTGTACTAGGTTCCTGCCATG
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 17
Experimental conditions
Panel name UniGene
Primer_f -
Primer_r -
PCR product length -
PCR conditions -
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