##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII AclI AgeI Alw44I ApaLI
AscI AseI AsnI BsaBI BsiWI
BspDI BspLU11I BsrFI BssSI BstBI
BstEII BstPI Cfr10I ClaI CpoI
DraI DraIII EcoO65I EcoRV FseI
HpaI MamI MfeI MunI NaeI
NdeI NgoMIV NheI NspV PacI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SbfI ScaI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccIII 1907 TCCGGA T^CCGGA
AflII 3060 CTTAAG C^TTAAG
AflIII 29 ACGCGT A^C[GA][CT]GT
AhdI 4908 GACAGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 4908 GACAGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 456 CCTAGG C^CTAGG
BfrI 3060 CTTAAG C^TTAAG
BlnI 456 CCTAGG C^CTAGG
BseAI 1907 TCCGGA T^CCGGA
BsiEI 2 CGGCCG CG[GA][CT]^CG
BsmBI 4663 CGTCTC CGTCTC(1/5)
Bsp1407I 3325 TGTACA T^GTACA
BspEI 1907 TCCGGA T^CCGGA
BsrGI 3325 TGTACA T^GTACA
BssHII 45 GCGCGC G^CGCGC
Bst1107I 2423 GTATAC GTA^TAC
BstZ17I 2423 GTATAC GTA^TAC
DrdI 5910 GACTGGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2 CGGCCG C^GGCCG
Eam1105I 4908 GACAGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2 CGGCCG C^GGCCG
Eco52I 2 CGGCCG C^GGCCG
EcoRI 309 GAATTC G^AATTC
EcoT22I 2273 ATGCAT ATGCA^T
HindIII 2436 AAGCTT A^AGCTT
MluI 29 ACGCGT A^CGCGT
MroI 1907 TCCGGA T^CCGGA
NotI 1 GCGGCCGC GC^GGCCGC
NruI 9 TCGCGA TCG^CGA
NsiI 2273 ATGCAT ATGCA^T
PaeR7I 4032 CTCGAG C^TCGAG
PshAI 3786 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 3266 GTCGAC G^TCGAC
SapI 3516 GAAGAGC GCTCTTC(1/4)
SfiI 158 GGCCCCGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 2905 GCATGC GCATG^C
SspBI 3325 TGTACA T^GTACA
StuI 4871 AGGCCT AGG^CCT
XhoI 4032 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AspI 1736 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2442 GACGCTGTC
AviII 2461 TGCGCA TGC^GCA
5729 TGCGCA
BamHI 142 GGATCC G^GATCC
2072 GGATCC
BbeI 2389 GGCGCC GGCGC^C
4407 GGCGCC
BbrPI 4331 CACGTG CAC^GTG
4434 CACGTG
BciVI 5679 GGATAC GTATCC(6/5)
1649 GTATCC
BsaWI 1907 TCCGGA [AT]^CCGG[AT]
4267 ACCGGA
BspHI 2509 TCATGA T^CATGA
5053 TCATGA
BsrDI 2372 CATTGC GCAATG(2/0)
4087 GCAATG
BstAPI 2275 GCATGATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5706 GCACAGCCTGC
FspI 2461 TGCGCA TGC^GCA
5729 TGCGCA
HincII 3266 GTCGAC GT[CT]^[GA]AC
4256 GTTGAC
HindII 3266 GTCGAC GT[CT]^[GA]AC
4256 GTTGAC
KasI 2389 GGCGCC G^GCGCC
4407 GGCGCC
KspI 794 CCGCGG CCGC^GG
5296 CCGCGG
NarI 2389 GGCGCC GG^CGCC
4407 GGCGCC
PflFI 1736 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2442 GACGCTGTC
PflMI 702 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4496 CCATGCTGTGG
PmaCI 4331 CACGTG CAC^GTG
4434 CACGTG
PmlI 4331 CACGTG CAC^GTG
4434 CACGTG
RcaI 2509 TCATGA T^CATGA
5053 TCATGA
SacI 2118 GAGCTC GAGCT^C
3810 GAGCTC
SacII 794 CCGCGG CCGC^GG
5296 CCGCGG
SexAI 601 ACCAGGT A^CC[AT]GGT
5474 ACCTGGT
SfoI 2389 GGCGCC GGC^GCC
4407 GGCGCC
Tth111I 1736 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2442 GACGCTGTC
Van91I 702 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4496 CCATGCTGTGG
XcmI 401 CCAAGAGCAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1198 CCAGGGATGATATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
Acc65I AccI AccII AciI AcsI
AcyI AfaI AluI AlwI AlwNI
AocI Aor51HI ApaI ApoI Asp700I
Asp718I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcgI BclI BcnI BfaI BglI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco47III Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KpnI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XhoII XmnI