Gene/Protein Characteristic Table for KIAA1476
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Product ID ORK04255
Accession No AB040909
Description bromodomain adjacent to zinc finger domain, 2B
Clone name fj04778s1
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (7985 bp)
Predicted protein sequence (2142 aa)
Source Human fetal brain
Rouge ID mKIAA1476 by Kazusa Mouse cDNA Project
Note We replaced fj04778, former representative clones for KIAA1476 with fj04778s1. (2003/4/2)
Features of the cloned cDNA sequence
Description

Length: 7985 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 1213 bp
Genome contig ID gi89161199r_159783809
PolyA signal sequence
(AATAAA,-22)
+----*----+----*----+----*----+----
AAGTTTTATTTAAAATAAAATGTTGTGGAAAAGGT
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
AGCATTCTTTTTTTAGGAGTGTTATTTTTCACTATGTGTGGCACGGATAC

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 2 r 159883809 160181326 36 99.8 Perfect prediction
Features of the protein sequence
Description

Length: 2142 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
XP_856656 0 96.0 similar to Brom...
Canis lupus fam...
XP_848667 0 94.5 similar to Brom...
Canis lupus fam...
XP_856693 0 92.0 similar to Brom...
Canis lupus fam...
CAM26914 0 84.4 bromodomain adj...
Mus musculus
EAX11404 0 98.3 bromodomain adj...
Homo sapiens
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB002312 1.2e-17 36.6 KIAA0314
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
FPrintScan IPR001487 2054 2067 PR00503 Bromodomain
IPR001487 2068 2084 PR00503 Bromodomain
IPR001487 2084 2102 PR00503 Bromodomain
IPR001487 2102 2121 PR00503 Bromodomain
HMMPfam IPR001739 747 796 PF01429 Methyl-CpG binding
IPR004022 1062 1123 PF02791 DDT
IPR001965 1907 1955 PF00628 Zinc finger
IPR001487 2038 2126 PF00439 Bromodomain
HMMSmart IPR001739 750 820 SM00391 Methyl-CpG binding
IPR004022 1061 1126 SM00571 DDT
IPR001965 1907 1953 SM00249 Zinc finger
IPR001487 2032 2140 SM00297 Bromodomain
ProfileScan IPR001739 747 822 PS50982 Methyl-CpG binding
IPR004022 1061 1126 PS50827 DDT
IPR001965 1905 1955 PS50016 Zinc finger
IPR001487 2051 2121 PS50014 Bromodomain
ScanRegExp IPR001487 2056 2113 PS00633 Bromodomain
Experimental conditions
Primer_f CATTATCAGAAGCTCGCAGTG
Primer_r CCCTTTCGACAGATTTGGCAG
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 2
Experimental conditions
Panel name GeneBridge 4
Primer_f TAGTCTTTTGCCACGAACACC
Primer_r AGATACTCCACCTTTCACCTG
PCR product length 202(600) bp
PCR conditions 95 °C15 sec64 °C60 sec30 cycles
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