Gene/Protein Characteristic Table for KIAA1400
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Product ID ORK01629
Accession No AB037821
Description protocadherin 10, transcript variant 1
Clone name hk08136
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (4554 bp)
Predicted protein sequence (1093 aa)
Flexi ORF Clone FXC01629
Source Human adult brain
Rouge ID mKIAA1400 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 4554 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 605 bp
Genome contig ID gi89161207f_134189920
PolyA signal sequence
(AATAAA,-23)
+----*----+----*----+----*----+----
TCAAAGCTTCTAAATAAATATAAAATATATATATT
Flanking genome sequence
(141452 - 141501)
----+----*----+----*----+----*----+----*----+----*
ATATTATATAATTTTCCTAAAATGTGGTACAACTCAGTTGGTTTTTAAAT

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 4 f 134289920 134331370 5 99.2 Perfect prediction
Features of the protein sequence
Description

Length: 1093 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
Q9P2E7 0 100.0 Protocadherin-1...
Homo sapiens
XP_001134989 0 99.9 protocadherin 1...
Pan troglodytes
AAI11561 0 99.9 Protocadherin 1...
Homo sapiens
XP_869875 0 98.7 similar to prot...
Bos taurus
AAK57195 0 97.4 OL-protocadheri...
Mus musculus
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB046782 2.1e-63 37.8 KIAA1562
AB011160 5.2e-62 40.0 KIAA0588
AB002343 4.5e-58 41.9 KIAA0345
AB002325 4.5e-27 43.9 KIAA0327
AB053445 2.3e-24 29.7 KIAA1774
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
FPrintScan IPR002126 115 134 PR00205 Cadherin
IPR002126 303 332 PR00205 Cadherin
IPR002126 481 493 PR00205 Cadherin
IPR002126 495 514 PR00205 Cadherin
IPR002126 514 527 PR00205 Cadherin
IPR002126 574 600 PR00205 Cadherin
IPR002126 609 626 PR00205 Cadherin
HMMPfam IPR013164 71 154 PF08266 Cadherin
IPR002126 180 264 PF00028 Cadherin
IPR002126 308 402 PF00028 Cadherin
IPR002126 420 507 PF00028 Cadherin
IPR002126 521 618 PF00028 Cadherin
IPR002126 647 732 PF00028 Cadherin
HMMSmart IPR002126 87 173 SM00112 Cadherin
IPR002126 197 301 SM00112 Cadherin
IPR002126 325 409 SM00112 Cadherin
IPR002126 433 514 SM00112 Cadherin
IPR002126 538 625 SM00112 Cadherin
IPR002126 656 739 SM00112 Cadherin
ProfileScan IPR002126 116 175 PS50268 Cadherin
IPR002126 176 303 PS50268 Cadherin
IPR002126 304 411 PS50268 Cadherin
IPR002126 412 516 PS50268 Cadherin
IPR002126 517 627 PS50268 Cadherin
IPR002126 643 733 PS50268 Cadherin
ScanRegExp IPR002126 163 173 PS00232 Cadherin
IPR002126 291 301 PS00232 Cadherin
IPR002126 399 409 PS00232 Cadherin
IPR002126 504 514 PS00232 Cadherin
IPR002126 615 625 PS00232 Cadherin
IPR013032 812 823 PS00022 EGF-like region

Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length
SOSUI2 1 19 ALLFVFFQIFFCFVVVGEVIGWL 41 PRIMARY 23
2 50 FLLEMIVLLLFALLWMVEGVFSQ 72 PRIMARY 23
3 768 TLILIIALGSVSFIFLLAMIVLA 790 PRIMARY 23
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f TGTCACTGAGCCCTTTAGATG
Primer_r TTGGAATAGCTTGCGGAACAC
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 4
Experimental conditions
Panel name GeneBridge 4
Primer_f TGTCACTGAGCCCTTTAGATG
Primer_r TTGGAATAGCTTGCGGAACAC
PCR product length 152 bp
PCR conditions 95 °C15 sec62 °C60 sec30 cycles
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