##################################
# NO CUT ENZYMES (   88 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AgeI        AhdI        Alw44I      Aor51HI     ApaI        
ApaLI       AscI        Asp700I     Asp718I     AspEI       
AviII       AvrII       BalI        BbrPI       BcgI        
BciVI       BclI        BlnI        BsaAI       BsaBI       
BsaWI       BseAI       BsiWI       BsmBI       BspDI       
BspEI       BspHI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     ClaI        DraIII      
DrdI        Eam1105I    Eco47III    EcoO65I     FbaI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        KpnI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MunI        NcoI        
NotI        NruI        NspV        PflMI       PinAI       
PmaCI       PmeI        PmlI        Psp1406I    PvuI        
RcaI        SacI        SalI        SbfI        SexAI       
SfiI        SfuI        SgrAI       SmaI        SnaBI       
Sse8387I    StuI        Van91I      XbaI        XcmI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AcyI          284  GGCGCC               G[GA]^CG[CT]C
AflII        3307  CTTAAG               C^TTAAG
AlwNI        3640  CAGCTACTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         2092  CCTCAGG              CC^T[ACGT]AGG
AspHI        2531  GAGCAC               G[AT]GC[AT]^C
AspI         2897  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        2958  GGATCC               G^GATCC
BbeI          284  GGCGCC               GGCGC^C
BfrI         3307  CTTAAG               C^TTAAG
BglI          115  GCCGGCCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1034  AGATCT               A^GATCT
BsaHI         284  GGCGCC               G[GA]^CG[CT]C
BsiEI          47  CGGCCG               CG[GA][CT]^CG
BsiHKAI      2531  GAGCAC               G[AT]GC[AT]^C
Bsp1407I     1805  TGTACA               T^GTACA
BspMI        3319  ACCTGC               ACCTGC(4/8)
BsrBI         199  CCGCTC               CCGCTC(-3/-3)
BsrGI        1805  TGTACA               T^GTACA
BssHII        276  GCGCGC               G^CGCGC
BssSI        2534  CACGAG               CACGAG(-5/-1)
BstXI        2152  CCAGTCGTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       2092  CCTCAGG              CC^T[ACGT]AGG
CpoI          125  CGGACCG              CG^G[AT]CCG
EagI           47  CGGCCG               C^GGCCG
EclXI          47  CGGCCG               C^GGCCG
Eco52I         47  CGGCCG               C^GGCCG
Eco81I       2092  CCTCAGG              CC^T[ACGT]AGG
EcoRI        3871  GAATTC               G^AATTC
HaeII         284  GGCGCC               [GA]GCGC^[CT]
HgaI         1358  GACGC                GACGC(5/10)
Hin1I         284  GGCGCC               G[GA]^CG[CT]C
KasI          284  GGCGCC               G^GCGCC
KspI          300  CCGCGG               CCGC^GG
MaeII         421  ACGT                 A^CGT
NarI          284  GGCGCC               GG^CGCC
NdeI         3411  CATATG               CA^TATG
PacI         3426  TTAATTAA             TTAAT^TAA
PaeR7I       2824  CTCGAG               C^TCGAG
PflFI        2897  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         136  GGGTCCC              [GA]G^G[AT]CC[CT]
PshAI        3728  GACCACTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII         125  CGGACCG              CG^G[AT]CCG
SacII         300  CCGCGG               CCGC^GG
SapI         1187  GAAGAGC              GCTCTTC(1/4)
ScaI         1873  AGTACT               AGT^ACT
SfoI          284  GGCGCC               GGC^GCC
SpeI         2978  ACTAGT               A^CTAGT
SphI         1523  GCATGC               GCATG^C
SspBI        1805  TGTACA               T^GTACA
SwaI         3371  ATTTAAAT             ATTT^AAAT
Tth111I      2897  GACCAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         2824  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AflIII       2866  ACATGT               A^C[GA][CT]GT
             3990  ACATGT              
AseI         2573  ATTAAT               AT^TAAT
             3293  ATTAAT              
AsnI         2573  ATTAAT               AT^TAAT
             3293  ATTAAT              
BanI          284  GGCGCC               G^G[CT][GA]CC
             1166  GGCACC              
BanII         295  GAGCCC               G[GA]GC[CT]^C
             1961  GGGCTC              
BbsI         2787  GTCTTC               GAAGAC(2/6)
             3994  GTCTTC              
BlpI         1963  GCTCAGC              GC^T[ACGT]AGC
             2528  GCTGAGC             
Bpu1102I     1963  GCTCAGC              GC^T[ACGT]AGC
             2528  GCTGAGC             
BpuAI        2787  GTCTTC               GAAGAC(2/6)
             3994  GTCTTC              
BsgI         3457  CTGCAC               GTGCAG(16/14)
              392  GTGCAG              
BspLU11I     2866  ACATGT               A^CATGT
             3990  ACATGT              
BsrFI          49  GCCGGC               [GA]^CCGG[CT]
              115  GCCGGC              
CelII        1963  GCTCAGC              GC^T[ACGT]AGC
             2528  GCTGAGC             
Cfr10I         49  GCCGGC               [GA]^CCGG[CT]
              115  GCCGGC              
DraII         136  GGGTCCC              [GA]G^G[ACGT]CC[CT]
             1692  GGGGCCT             
DsaI          300  CCGCGG               C^C[GA][CT]GG
              353  CCGTGG              
EaeI           47  CGGCCG               [CT]^GGCC[GA]
              350  TGGCCG              
EcoNI         320  CCTGGAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3999  CCTTTTTGAGG         
EcoO109I      136  GGGTCCC              [GA]G^G[ACGT]CC[CT]
             1692  GGGGCCT             
EcoRV        1278  GATATC               GAT^ATC
             3355  GATATC              
EcoT14I      2274  CCAAGG               C^C[AT][AT]GG
             2654  CCTTGG              
EcoT22I       699  ATGCAT               ATGCA^T
             3170  ATGCAT              
NaeI           49  GCCGGC               GCC^GGC
              115  GCCGGC              
NgoMIV         49  GCCGGC               G^CCGGC
              115  GCCGGC              
NheI         1361  GCTAGC               G^CTAGC
             2268  GCTAGC              
NsiI          699  ATGCAT               ATGCA^T
             3170  ATGCAT              
PshBI        2573  ATTAAT               AT^TAAT
             3293  ATTAAT              
SmlI         2824  CTCGAG               C^T[CT][GA]AG
             3307  CTTAAG              
SspI         2622  AATATT               AAT^ATT
             3528  AATATT              
StyI         2274  CCAAGG               C^C[AT][AT]GG
             2654  CCTTGG              


#########################################
# 3 OR MORE CUT ENZYMES (   88 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        ApoI        AvaI        AvaII       BbvCI       
BbvI        BcnI        BfaI        BmyI        BpmI        
BsaI        BsaJI       BseRI       BsiYI       BslI        
BsmAI       BsmFI       BsmI        BsoBI       Bsp1286I    
BsrDI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraI        EarI        EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PleI        PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII