Gene/Protein Characteristic Table for KIAA1244
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Product ID ORK00777
Accession No AB033070
Description ARFGEF family member 3
Clone name hf00438s1
Vector information
The cDNA fragment was originally inserted at SalI-NotI site ...
cDNA sequence DNA sequence (7932 bp)
Predicted protein sequence (1872 aa)
Flexi ORF Clone FXC00777
Source Human adult brain
Rouge ID mKIAA1244 by Kazusa Mouse cDNA Project
Note We replaced hf00438, former representative clones for KIAA1244 with hf00438s1. (2002/5/10)
Features of the cloned cDNA sequence
Description

Length: 7932 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning No warning
Integrity of 3' end
Length of 3'UTR 2313 bp
Genome contig ID gi89161210f_138518411
PolyA signal sequence
(None)
+----*----+----*----+----*----+----
ACTCCAGACTGGGTGACAAGAGTGAAACTCCATCT
Flanking genome sequence
(183220 - 183269)
----+----*----+----*----+----*----+----*----+----*
AAAAAAAAAAAAAAAAAAAAGTGAATACTGTATCCCAAAGTATGTTAGTT

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 6 f 138618411 138701629 25 100.0 Perfect prediction
Features of the protein sequence
Description

Length: 1872 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
XP_518767 0 99.8 hypothetical pr...
Pan troglodytes
XP_001095348 0 98.9 similar to CG59...
Macaca mulatta
BAG10007 0 100.0 brefeldin A-inh...
synthetic construct
AAL04174 0 100.0 BIG3 [Homo sapi...
Homo sapiens
Q3UGY8 0 93.0 Brefeldin A-inh...
Mus musculus
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR015403 938 1014 PF09324 Protein of unknown function DUF1981
HMMSmart IPR000904 281 493 SM00222 SEC7-like

Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length
SOSUI2 1 5 TAPALSGPVARTIYYIAAELVRL 27 SECONDARY 23
2 114 GIEACYAAVSCVCTLLGALDELS 136 PRIMARY 23
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f TTGAGGAGCTGAAGGATGGGG
Primer_r TGTCAAGGCTCCTCTCGTTCC
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 6
Experimental conditions
Panel name GeneBridge 4
Primer_f TTGAGGAGCTGAAGGATGGGG
Primer_r TGTCAAGGCTCCTCTCGTTCC
PCR product length 135 bp
PCR conditions 95 °C15 sec68 °C60 sec30 cycles
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