##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
Acc65I AccIII AclI AflII Alw44I
ApaLI AscI Asp718I AviII BciVI
BfrI BglII BsaBI BseAI BsiWI
Bsp1407I BspEI BsrGI BssHII BssSI
BstBI BstEII BstPI CpoI EcoO65I
EcoRI FseI FspI HincII HindII
HpaI KpnI MamI MluI MroI
NdeI NheI NotI NruI NspV
PacI PmeI Psp1406I PvuI RsrII
SalI SbfI SfiI SfuI SgrAI
SpeI Sse8387I SspBI SwaI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AatII 2899 GACGTC GACGT^C
AhdI 970 GACATTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2230 AGCGCT AGC^GCT
ApaI 3870 GGGCCC GGGCC^C
AseI 6072 ATTAAT AT^TAAT
AsnI 6072 ATTAAT AT^TAAT
AspEI 970 GACATTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1026 CCTAGG C^CTAGG
BamHI 5076 GGATCC G^GATCC
BlnI 1026 CCTAGG C^CTAGG
BspDI 1702 ATCGAT AT^CGAT
BspLU11I 2754 ACATGT A^CATGT
Bst1107I 7375 GTATAC GTA^TAC
BstXI 1748 CCAAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 7375 GTATAC GTA^TAC
ClaI 1702 ATCGAT AT^CGAT
DrdI 2908 GACCAGGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 970 GACATTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2230 AGCGCT AGC^GCT
EcoT22I 327 ATGCAT ATGCA^T
KspI 2382 CCGCGG CCGC^GG
NsiI 327 ATGCAT ATGCA^T
PaeR7I 631 CTCGAG C^TCGAG
PshBI 6072 ATTAAT AT^TAAT
SacII 2382 CCGCGG CCGC^GG
SapI 1003 GAAGAGC GCTCTTC(1/4)
SnaBI 6368 TACGTA TAC^GTA
SphI 325 GCATGC GCATG^C
XhoI 631 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AgeI 1034 ACCGGT A^CCGGT
3427 ACCGGT
Asp700I 1380 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3091 GAAGCTTTTC
AspI 834 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2341 GACCTCGTC
BbeI 1365 GGCGCC GGCGC^C
4489 GGCGCC
BcgI 4325 CGATACAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1820 GCATGAGCCTCG
BsaI 2973 GAGACC GGTCTC(1/5)
7731 GAGACC
BsaWI 1034 ACCGGT [AT]^CCGG[AT]
3427 ACCGGT
BsiEI 1354 CGGCCG CG[GA][CT]^CG
4583 CGGCCG
BspHI 1388 TCATGA T^CATGA
2396 TCATGA
BstAPI 438 GCAACTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
6015 GCAATATCTGC
EagI 1354 CGGCCG C^GGCCG
4583 CGGCCG
EclXI 1354 CGGCCG C^GGCCG
4583 CGGCCG
Eco52I 1354 CGGCCG C^GGCCG
4583 CGGCCG
EcoNI 2724 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4605 CCTTTTTGAGG
EcoRV 3736 GATATC GAT^ATC
7356 GATATC
KasI 1365 GGCGCC G^GCGCC
4489 GGCGCC
MfeI 3483 CAATTG C^AATTG
6515 CAATTG
MunI 3483 CAATTG C^AATTG
6515 CAATTG
NaeI 18 GCCGGC GCC^GGC
3343 GCCGGC
NarI 1365 GGCGCC GG^CGCC
4489 GGCGCC
NgoMIV 18 GCCGGC G^CCGGC
3343 GCCGGC
PflFI 834 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2341 GACCTCGTC
PinAI 1034 ACCGGT A^CCGGT
3427 ACCGGT
PstI 828 CTGCAG CTGCA^G
1183 CTGCAG
RcaI 1388 TCATGA T^CATGA
2396 TCATGA
SfoI 1365 GGCGCC GGC^GCC
4489 GGCGCC
Tth111I 834 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2341 GACCTCGTC
XbaI 5807 TCTAGA T^CTAGA
7215 TCTAGA
XmnI 1380 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3091 GAAGCTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 146 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI ApoI AspHI AvaI AvaII
BalI BanI BanII BbrPI BbsI
BbvCI BbvI BclI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PmaCI
PmlI PpuMI PshAI PvuII RsaI
SacI Sau3AI Sau96I ScaI ScrFI
SexAI SfaNI SfcI SmaI SmlI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI