Gene/Protein Characteristic Table for KIAA0997
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Product ID ORK00711
Accession No AB023214
Description zinc finger and BTB domain containing 1, transcript variant 2
Clone name hk08613
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (3990 bp)
Predicted protein sequence (646 aa)
Flexi ORF Clone FXC00711
Source Human adult brain
Features of the cloned cDNA sequence
Description

Length: 3990 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 1793 bp
Genome contig ID gi51511730f_63941174
PolyA signal sequence
(AATAAA,-31)
+----*----+----*----+----*----+----
ACCTAATAAAATTTCCTCCTCTATATTCCAAAGTT
Flanking genome sequence
(128989 - 129038)
----+----*----+----*----+----*----+----*----+----*
AAATTGTCTGTATATTACTTTTATTTATACTGTATTTTTTCCAATCTGGC

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 14 f 64041174 64070161 3 99.1 Perfect prediction
Features of the protein sequence
Description

Length: 646 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
AAT06732 0 100.0 L2 [Homo sapiens].
Homo sapiens
EAW80863 0 99.8 zinc finger and...
Homo sapiens
XP_001499240 0 97.5 zinc finger and...
Equus caballus
Q9Y2K1 0 99.8 Zinc finger and...
Homo sapiens
AAH50719 0 99.7 ZBTB1 protein [...
Homo sapiens
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB002352 1e-05 20.4 KIAA0354
AB028943 0.00011 24.0 KIAA1020
AB007901 0.00012 21.4 KIAA0441
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR013069 16 122 PF00651 BTB/POZ
IPR007087 536 558 PF00096 Zinc finger
IPR007087 608 630 PF00096 Zinc finger
HMMSmart IPR000210 26 123 SM00225 BTB/POZ-like
IPR015880 218 244 SM00355 Zinc finger
IPR015880 423 445 SM00355 Zinc finger
IPR015880 536 556 SM00355 Zinc finger
IPR015880 580 602 SM00355 Zinc finger
IPR015880 608 630 SM00355 Zinc finger
ProfileScan IPR000210 26 93 PS50097 BTB/POZ-like
IPR007087 580 607 PS50157 Zinc finger
IPR007087 608 635 PS50157 Zinc finger
ScanRegExp IPR007087 582 602 PS00028 Zinc finger
IPR007087 610 630 PS00028 Zinc finger
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f GTCCTTCAGCAGCTAAACAAC
Primer_r TAGAACTGCCTTGTGTGCTTG
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 14
Experimental conditions
Panel name CCR
Primer_f GTCCTTCAGCAGCTAAACAAC
Primer_r TAGAACTGCCTTGTGTGCTTG
PCR product length 99 bp
PCR conditions 95 °C15 sec62 °C60 sec30 cycles
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