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Order Kazusa clone(s) from : |
| Product ID | ORK00578 |
|---|---|
| Accession No | AB014520 |
| Description | plexin D1 |
| Clone name | hg04174 |
| Vector information | |
| cDNA sequence | DNA sequence (6753 bp) Predicted protein sequence (1984 aa) |
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HaloTag ORF Clone |
FHC00578
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| Flexi ORF Clone | FXC00578 |
| Source | Human adult brain |
| Rouge ID |
mKIAA0620
by Kazusa Mouse cDNA Project
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Length: 6753 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | Warning | No warning |
Length: 1984 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| HMMPfam | IPR001627 | 119 | 589 | PF01403 | Semaphorin/CD100 antigen |
| IPR002165 | 608 | 661 | PF01437 | Plexin | |
| IPR002165 | 761 | 813 | PF01437 | Plexin | |
| IPR002909 | 951 | 1038 | PF01833 | Cell surface receptor IPT/TIG | |
| IPR002909 | 1041 | 1125 | PF01833 | Cell surface receptor IPT/TIG | |
| IPR002909 | 1129 | 1210 | PF01833 | Cell surface receptor IPT/TIG | |
| IPR013548 | 1404 | 1948 | PF08337 | Plexin cytoplasmic region | |
| HMMSmart | IPR001627 | 119 | 589 | SM00630 | Semaphorin/CD100 antigen |
| IPR003659 | 608 | 661 | SM00423 | Plexin/semaphorin/integrin | |
| IPR003659 | 761 | 813 | SM00423 | Plexin/semaphorin/integrin | |
| IPR003659 | 908 | 949 | SM00423 | Plexin/semaphorin/integrin | |
| IPR002909 | 950 | 1039 | SM00429 | Cell surface receptor IPT/TIG | |
| IPR002909 | 1040 | 1126 | SM00429 | Cell surface receptor IPT/TIG | |
| IPR002909 | 1128 | 1207 | SM00429 | Cell surface receptor IPT/TIG | |
| ProfileScan | IPR001627 | 96 | 606 | PS51004 | Semaphorin/CD100 antigen |
Prediction of transmembrane (TM) segments| Method | No. | N terminal | transmembrane region | C terminal | type | length | SOSUI2 | 1 | 1329 | ETAIIVSIVICSVLLLLSVVALF | 1351 | PRIMARY | 23 |
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RT-PCR
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RT-PCR-ELISA
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Experimental conditions| Primer_f | CAGATTTTTGTTGCTTGGGCG |
|---|---|
| Primer_r | TTTCAGCAAGATCAAGTAGCC |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. 3
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | CAGATTTTTGTTGCTTGGGCG |
| Primer_r | TTTCAGCAAGATCAAGTAGCC |
| PCR product length | 196 bp |
| PCR conditions | 95 °C 15 sec 62 °C 60 sec 30 cycles |