##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AhdI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvrII BclI
BfrI BlnI BsaI BseAI BsiWI
BspEI BspLU11I BsrDI Bst1107I BstBI
BstZ17I CpoI DrdI Eam1105I EcoRI
EcoRV EcoT22I FbaI FseI HpaI
KpnI MfeI MroI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PmeI PshBI
Psp1406I PvuI RsrII SbfI SfuI
SnaBI SpeI Sse8387I SspI SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AccI 4996 GTCGAC GT^[AC][GT]AC
AgeI 820 ACCGGT A^CCGGT
Aor51HI 5687 AGCGCT AGC^GCT
AviII 3257 TGCGCA TGC^GCA
BamHI 6463 GGATCC G^GATCC
BbrPI 2212 CACGTG CAC^GTG
BbvCI 3637 GCTGAGG CCTCAGC(-5/-2)
BciVI 5360 GTATCC GTATCC(6/5)
BfaI 760 CTAG C^TAG
BsmI 5739 GAATGC GAATGC(1/-1)
Bsp1407I 4685 TGTACA T^GTACA
BspDI 2008 ATCGAT AT^CGAT
BspHI 4565 TCATGA T^CATGA
BsrGI 4685 TGTACA T^GTACA
ClaI 2008 ATCGAT AT^CGAT
DraI 6720 TTTAAA TTT^AAA
Eco47III 5687 AGCGCT AGC^GCT
EcoNI 5340 CCTGTTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 3257 TGCGCA TGC^GCA
MaeI 760 CTAG C^TAG
PinAI 820 ACCGGT A^CCGGT
PmaCI 2212 CACGTG CAC^GTG
PmlI 2212 CACGTG CAC^GTG
RcaI 4565 TCATGA T^CATGA
SacI 3211 GAGCTC GAGCT^C
SalI 4996 GTCGAC G^TCGAC
SexAI 2606 ACCAGGT A^CC[AT]GGT
SfiI 450 GGCCGAGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1158 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 4685 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AatII 1788 GACGTC GACGT^C
4993 GACGTC
AocI 2425 CCTCAGG CC^T[ACGT]AGG
3289 CCTGAGG
Asp700I 5193 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5775 GAATGAGTTC
BcgI 4148 GCAAAGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5253 GCAGAGCCATCG
Bsu36I 2425 CCTCAGG CC^T[ACGT]AGG
3289 CCTGAGG
Eco81I 2425 CCTCAGG CC^T[ACGT]AGG
3289 CCTGAGG
HindIII 5083 AAGCTT A^AGCTT
6724 AAGCTT
MluI 743 ACGCGT A^CGCGT
1067 ACGCGT
PshAI 2770 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4920 GACACAGGTC
ScaI 4550 AGTACT AGT^ACT
5393 AGTACT
SphI 713 GCATGC GCATG^C
1685 GCATGC
XmnI 5193 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5775 GAATGAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 153 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaI
ApaLI AspHI AvaI AvaII BalI
BanI BanII BbeI BbsI BbvI
BcnI BglI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssHII BssKI
BssSI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EagI EarI EclXI Eco52I EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeII MaeIII MamI
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacII SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI