Gene/Protein Characteristic Table for KIAA1550
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Product ID ORK06405
Accession No AB046770
Description plexin A4
Clone name ff03434
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (11518 bp)
Predicted protein sequence (1462 aa)
Source Human fetal brain
Rouge ID mKIAA1550 by Kazusa Mouse cDNA Project
Note We replaced fh16159 and fh16159s1, former representative clones for KIAA1550 with ff03434. (2002/5/10,2005/08/06)
Features of the cloned cDNA sequence
Description

Length: 11518 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning Warning
Integrity of 3' end
Length of 3'UTR 7127 bp
Genome contig ID gi89161213r_131358640
PolyA signal sequence
(AATAAA,-11)
+----*----+----*----+----*----+----
ATAGAATAAAGTTTATTAAAAAATAATAAAAATGC
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
AGTTTGGGATTTTGGGTGCTTCTTTATGTGACTGGCTAGGAGGGGCTGGG

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 7 r 131458640 131824667 30 99.4 Perfect prediction
Features of the protein sequence
Description

Length: 1462 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
Q9HCM2 0 100.0 Plexin-A4; Flag...
Homo sapiens
XP_519389 0 99.5 plexin A4, A is...
Pan troglodytes
XP_001136153 0 99.6 plexin A4, A is...
Pan troglodytes
XP_001498318 0 98.6 plexin A4 [Equu...
Equus caballus
XP_001135989 0 98.4 plexin A4, A is...
Pan troglodytes
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB007932 0 70.8 KIAA0463
AB033032 1.7e-85 35.6 KIAA1206
AB002313 6.9e-56 35.4 KIAA0315
AB014520 5.4e-50 36.1 KIAA0620
AB007867 1.2e-43 32.6 KIAA0407
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR001627 1 58 PF01403 Semaphorin/CD100 antigen
IPR002165 77 127 PF01437 Plexin
IPR002165 223 270 PF01437 Plexin
IPR002165 371 424 PF01437 Plexin
IPR002909 426 519 PF01833 Cell surface receptor IPT/TIG
IPR002909 522 604 PF01833 Cell surface receptor IPT/TIG
IPR002909 608 706 PF01833 Cell surface receptor IPT/TIG
IPR002909 710 797 PF01833 Cell surface receptor IPT/TIG
IPR013548 878 1432 PF08337 Plexin cytoplasmic region
HMMSmart IPR003659 77 127 SM00423 Plexin/semaphorin/integrin
IPR003659 223 270 SM00423 Plexin/semaphorin/integrin
IPR003659 371 424 SM00423 Plexin/semaphorin/integrin
IPR002909 425 519 SM00429 Cell surface receptor IPT/TIG
IPR002909 521 605 SM00429 Cell surface receptor IPT/TIG
IPR002909 607 707 SM00429 Cell surface receptor IPT/TIG
IPR002909 709 798 SM00429 Cell surface receptor IPT/TIG
ProfileScan IPR001627 1 75 PS51004 Semaphorin/CD100 antigen

Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length
SOSUI2 1 805 PAIVSIAVAGGLLIIFIVAVLIA 827 PRIMARY 23
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f CTAGAGATGAAGGTGTCGGTG
Primer_r ATCTGGGGGTTCTGTATGAGG
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 7
Experimental conditions
Panel name CCR
Primer_f CTAGAGATGAAGGTGTCGGTG
Primer_r ATCTGGGGGTTCTGTATGAGG
PCR product length 176(2k) bp
PCR conditions 95 °C15 sec64 °C60 sec30 cycles
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