##################################
# NO CUT ENZYMES ( 51 enzymes) #
##################################
AatII Acc65I AclI AgeI Aor51HI
ApaI AscI Asp718I AviII AvrII
BamHI BlnI BsaBI BsaI BsiWI
BspDI BsrDI Bst1107I BstEII BstPI
BstZ17I ClaI CpoI DraIII DrdI
Eco47III EcoO65I EcoRV FseI FspI
HpaI KpnI MamI MluI NheI
NotI NruI PinAI PmeI Psp1406I
PvuI RsrII SacI SalI SbfI
SgrAI SnaBI SpeI SphI Sse8387I
SwaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccIII 284 TCCGGA T^CCGGA
AhdI 156 GACCTCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 526 CCTCAGG CC^T[ACGT]AGG
Asp700I 4912 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 156 GACCTCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 2192 CACGTG CAC^GTG
BbvCI 2218 CCTCAGC CCTCAGC(-5/-2)
BglII 3072 AGATCT A^GATCT
BseAI 284 TCCGGA T^CCGGA
BsiEI 4751 CGGCCG CG[GA][CT]^CG
BsmBI 5228 GAGACG CGTCTC(1/5)
BspEI 284 TCCGGA T^CCGGA
BspHI 2348 TCATGA T^CATGA
BsrBI 215 GAGCGG CCGCTC(-3/-3)
BssHII 199 GCGCGC G^CGCGC
BstBI 4378 TTCGAA TT^CGAA
BstXI 1401 CCAACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 526 CCTCAGG CC^T[ACGT]AGG
DraII 632 AGGACCC [GA]G^G[ACGT]CC[CT]
EagI 4751 CGGCCG C^GGCCG
Eam1105I 156 GACCTCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 4751 CGGCCG C^GGCCG
Eco52I 4751 CGGCCG C^GGCCG
Eco81I 526 CCTCAGG CC^T[ACGT]AGG
EcoNI 450 CCTCAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 632 AGGACCC [GA]G^G[ACGT]CC[CT]
KspI 144 CCGCGG CCGC^GG
MroI 284 TCCGGA T^CCGGA
NaeI 258 GCCGGC GCC^GGC
NdeI 2792 CATATG CA^TATG
NgoMIV 258 GCCGGC G^CCGGC
NspV 4378 TTCGAA TT^CGAA
PacI 6737 TTAATTAA TTAAT^TAA
PaeR7I 3862 CTCGAG C^TCGAG
PmaCI 2192 CACGTG CAC^GTG
PmlI 2192 CACGTG CAC^GTG
PpuMI 632 AGGACCC [GA]G^G[AT]CC[CT]
RcaI 2348 TCATGA T^CATGA
SacII 144 CCGCGG CCGC^GG
SapI 2223 GCTCTTC GCTCTTC(1/4)
ScaI 1664 AGTACT AGT^ACT
SexAI 3182 ACCTGGT A^CC[AT]GGT
SfiI 248 GGCCGGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 4378 TTCGAA TT^CGAA
XbaI 4996 TCTAGA T^CTAGA
XhoI 3862 CTCGAG C^TCGAG
XmnI 4912 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 2758 GTCTAC GT^[AC][GT]AC
5100 GTAGAC
AcyI 219 GGCGCC G[GA]^CG[CT]C
674 GGCGCC
AflII 862 CTTAAG C^TTAAG
1138 CTTAAG
Alw44I 409 GTGCAC G^TGCAC
6831 GTGCAC
ApaLI 409 GTGCAC G^TGCAC
6831 GTGCAC
AspI 4338 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
6597 GACTGTGTC
BbeI 219 GGCGCC GGCGC^C
674 GGCGCC
BcgI 461 GCACCAAATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2679 GCAAGCCATTCG
BciVI 2505 GGATAC GTATCC(6/5)
4681 GTATCC
BfrI 862 CTTAAG C^TTAAG
1138 CTTAAG
BsaHI 219 GGCGCC G[GA]^CG[CT]C
674 GGCGCC
BsrFI 103 ACCGGC [GA]^CCGG[CT]
258 GCCGGC
BstAPI 411 GCACGCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3426 GCAGCAGCTGC
Cfr10I 103 ACCGGC [GA]^CCGG[CT]
258 GCCGGC
EcoRI 3874 GAATTC G^AATTC
4192 GAATTC
Hin1I 219 GGCGCC G[GA]^CG[CT]C
674 GGCGCC
HincII 3510 GTTGAC GT[CT]^[GA]AC
4512 GTCAAC
HindII 3510 GTTGAC GT[CT]^[GA]AC
4512 GTCAAC
HindIII 2810 AAGCTT A^AGCTT
5345 AAGCTT
KasI 219 GGCGCC G^GCGCC
674 GGCGCC
NarI 219 GGCGCC GG^CGCC
674 GGCGCC
NcoI 1518 CCATGG C^CATGG
4318 CCATGG
PflFI 4338 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
6597 GACTGTGTC
PshAI 3513 GACGGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3646 GACAAAAGTC
PstI 1493 CTGCAG CTGCA^G
3811 CTGCAG
PvuII 1394 CAGCTG CAG^CTG
3430 CAGCTG
SfoI 219 GGCGCC GGC^GCC
674 GGCGCC
SmaI 170 CCCGGG CCC^GGG
357 CCCGGG
StuI 2356 AGGCCT AGG^CCT
4050 AGGCCT
Tth111I 4338 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
6597 GACTGTGTC
XmaCI 170 CCCGGG C^CCGGG
357 CCCGGG
XmaI 170 CCCGGG C^CCGGG
357 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AseI
AsnI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BclI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspLU11I BspMI BsrGI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DsaI
EaeI EarI EcoRII EcoT14I EcoT22I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MunI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PshBI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII