Gene/Protein Characteristic Table for KIAA0544
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Product ID ORK00557
Accession No AB011116
Description mahogunin ring finger 1, E3 ubiquitin protein ligase, transcript variant 3
Clone name hg04431
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (6450 bp)
Predicted protein sequence (583 aa)
Flexi ORF Clone FXC00557
Source Human adult brain
Rouge ID mKIAA0544 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 6450 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning No warning
Integrity of 3' end
Length of 3'UTR 4698 bp
Genome contig ID gi51511732f_4514870
PolyA signal sequence
(AATAAA,-19)
+----*----+----*----+----*----+----
ACTGTATATTTATAGTAATAAAATCATGCAGCAAT
Flanking genome sequence
(166107 - 166156)
----+----*----+----*----+----*----+----*----+----*
ATCCTGTCTGCTCCTTCCTCCGGGTGCAGCCCTCAGGATTGTGGCTGTTT

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 16 f 4614870 4680975 19 99.1 Internal No-hit
Features of the protein sequence
Description

Length: 583 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
O60291 8.5e-213 100.0 Probable E3 ubi...
Homo sapiens
XP_510786 7.6e-211 96.0 mahogunin, ring...
Pan troglodytes
AAH50389 2.3e-207 100.0 Mahogunin, ring...
Homo sapiens
XP_001367486 4.7e-191 89.0 similar to Prob...
Monodelphis dom...
NP_001070410 2.1e-182 87.9 mahogunin, ring...
Bos taurus
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB067504 6.4e-66 57.2 KIAA1917
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR001841 309 347 PF00097 Zinc finger
HMMSmart IPR001841 309 347 SM00184 Zinc finger
ProfileScan IPR001841 309 348 PS50089 Zinc finger
Expression profile
Description

RT-PCR
Experimental conditions
Primer_f CTTTCCATCCCTAGTTCAGAG
Primer_r GCTTCGACCACCAATCAGTTC
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 16
Experimental conditions
Panel name GeneBridge 4
Primer_f CTTTCCATCCCTAGTTCAGAG
Primer_r GCTTCGACCACCAATCAGTTC
PCR product length 172 bp
PCR conditions 95 °C15 sec64 °C60 sec30 cycles
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