KIAA0520

Product ID	ORK01670
Accession No	AB011092
Description	adenylate cyclase 9
Clone name	hg02989s1
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (7544 bp) Predicted protein sequence (1364 aa)
HaloTag ORF Clone	FHC01670
Flexi ORF Clone	FXC01670
Source	Human adult brain
Rouge ID	mKIAA0520 by Kazusa Mouse cDNA Project
Note	We replaced hg01313 and hg02989, former representative clones for KIAA0520 with hg02989s1. (2002/5/10,2005/08/06)

Length: 7544 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	3102 bp
Genome contig ID	gi51511732r_3852654
PolyA signal sequence (ATTAAA,-24)	+--------+--------+----*----+---- AAGAAATGGACATTAAAATATTCTAAAATTTAAAT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ATATGATGCCTGGTGTTTTCTTATTGAAAAGATGAGCACTGCTTCAGAGA

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	16	r	3952654	4106029	12	99.2	Perfect prediction

Length: 1364 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
AAI51230	100.0	ADCY9 protein [...	Homo sapiens
O60503	99.9	Adenylate cycla...	Homo sapiens
AAY27880	99.9	adenylyl cyclas...	Homo sapiens
CAB65084	99.8	adenylyl cyclas...	Homo sapiens
XP_510775	97.8	adenylate cycla...	Pan troglodytes

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB007882	1.7e-35	28.9	KIAA0422
AB011083	5.7e-33	31.2	KIAA0511
D25538	5.1e-32	30.2	KIAA0037
AB028983	1.9e-29	32.3	KIAA1060

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR001054	396	584	PF00211	Adenylyl cyclase class-3/4/guanylyl cyclase
HMMPfam	IPR001054	1060	1255	PF00211	Adenylyl cyclase class-3/4/guanylyl cyclase
HMMSmart	IPR001054	336	558	SM00044	Adenylyl cyclase class-3/4/guanylyl cyclase
HMMSmart	IPR001054	1034	1238	SM00044	Adenylyl cyclase class-3/4/guanylyl cyclase
ProfileScan	IPR001054	405	532	PS50125	Adenylyl cyclase class-3/4/guanylyl cyclase
ProfileScan	IPR001054	1069	1209	PS50125	Adenylyl cyclase class-3/4/guanylyl cyclase
ScanRegExp	IPR001054	509	532	PS00452	Adenylyl cyclase class-3/4/guanylyl cyclase
ScanRegExp	IPR001054	1186	1209	PS00452	Adenylyl cyclase class-3/4/guanylyl cyclase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	128	RYALFYIGFACLLWSIYFAVHMR	150	PRIMARY	23
	2	154	IVMVAPALCFLLVCVGFFLFTFT	176	PRIMARY	23
	3	187	SLALTLLVFALTLAAQFQVLTPV	209	SECONDARY	23
	4	242	LFLLYTVMHLPLYLSLCLGVAYS	264	PRIMARY	23
	5	295	RGLLHGCIHAIGVHLFVMSQVR	316	SECONDARY	22
	6	796	FSSLLDVFLSTTVFLTLSTTCFL	818	SECONDARY	23
	7	840	LLEVLSLAVSIRMVFFLEDVMAC	862	PRIMARY	23
	8	876	RHCIGAILVSLPALAVYSHVTSE	898	PRIMARY	23
	9	902	NIHFPVFTGSAALIAVVHYCNFC	924	SECONDARY	23
	10	930	MRSSLATVVGAGPLLLLYVSLCP	952	PRIMARY	23
	11	986	ASLIGQEVVLVFFLLLLLVWFLN	1008	PRIMARY	23

Primer_f	ACAGTCGGTTCTTGCTAATTC
Primer_r	TGCTTCCTGTCACATGGGCTG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	ACAGTCGGTTCTTGCTAATTC
Primer_r	TGCTTCCTGTCACATGGGCTG
PCR product length	157 bp
PCR conditions	95 °C15 sec66 °C60 sec30 cycles