##################################
# NO CUT ENZYMES ( 36 enzymes) #
##################################
Acc65I AscI Asp700I Asp718I BamHI
BciVI BsaBI BsiWI Bsp1407I BspDI
BspLU11I BsrGI Bst1107I BstBI BstZ17I
ClaI CpoI HindIII HpaI KpnI
MamI NdeI NheI NotI NspV
PacI PmeI PvuI RsrII ScaI
SfiI SfuI SnaBI SpeI SspBI
XmnI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AflII 2259 CTTAAG C^TTAAG
AseI 2619 ATTAAT AT^TAAT
AsnI 2619 ATTAAT AT^TAAT
AvrII 2187 CCTAGG C^CTAGG
BcgI 142 GCAGGCGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1786 TGATCA T^GATCA
BfrI 2259 CTTAAG C^TTAAG
BlnI 2187 CCTAGG C^CTAGG
BspHI 4956 TCATGA T^CATGA
BsrDI 5036 CATTGC GCAATG(2/0)
EcoRI 1445 GAATTC G^AATTC
FbaI 1786 TGATCA T^GATCA
FseI 57 GGCCGGCC GGCCGG^CC
KspI 7225 CCGCGG CCGC^GG
MfeI 7027 CAATTG C^AATTG
MluI 895 ACGCGT A^CGCGT
MunI 7027 CAATTG C^AATTG
NcoI 3884 CCATGG C^CATGG
NruI 3371 TCGCGA TCG^CGA
PshAI 965 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2619 ATTAAT AT^TAAT
RcaI 4956 TCATGA T^CATGA
SacII 7225 CCGCGG CCGC^GG
SalI 2759 GTCGAC G^TCGAC
SapI 737 GCTCTTC GCTCTTC(1/4)
SbfI 97 CCTGCAGG CCTGCA^GG
SexAI 4219 ACCTGGT A^CC[AT]GGT
Sse8387I 97 CCTGCAGG CCTGCA^GG
SwaI 7537 ATTTAAAT ATTT^AAAT
XbaI 7341 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AatII 2919 GACGTC GACGT^C
7217 GACGTC
AccI 2759 GTCGAC GT^[AC][GT]AC
3018 GTCTAC
AccIII 187 TCCGGA T^CCGGA
7258 TCCGGA
AclI 4422 AACGTT AA^CGTT
6703 AACGTT
AgeI 2002 ACCGGT A^CCGGT
5628 ACCGGT
AhdI 802 GACTGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1902 GACGTGTGGTC
AocI 2179 CCTCAGG CC^T[ACGT]AGG
5372 CCTCAGG
Aor51HI 164 AGCGCT AGC^GCT
697 AGCGCT
ApaI 1204 GGGCCC GGGCC^C
3888 GGGCCC
AspEI 802 GACTGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1902 GACGTGTGGTC
BbrPI 3495 CACGTG CAC^GTG
4674 CACGTG
BglII 2461 AGATCT A^GATCT
4794 AGATCT
BseAI 187 TCCGGA T^CCGGA
7258 TCCGGA
BsmBI 3188 CGTCTC CGTCTC(1/5)
3409 GAGACG
BsmI 4952 GCATTC GAATGC(1/-1)
7500 GCATTC
BspEI 187 TCCGGA T^CCGGA
7258 TCCGGA
BsrBI 5678 CCGCTC CCGCTC(-3/-3)
5104 GAGCGG
BssHII 65 GCGCGC G^CGCGC
213 GCGCGC
BstEII 343 GGTGACC G^GT[ACGT]ACC
5419 GGTAACC
BstPI 343 GGTGACC G^GT[ACGT]ACC
5419 GGTAACC
Bsu36I 2179 CCTCAGG CC^T[ACGT]AGG
5372 CCTCAGG
DrdI 4161 GACATCCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6530 GACCTTGCTGTC
Eam1105I 802 GACTGATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1902 GACGTGTGGTC
Eco47III 164 AGCGCT AGC^GCT
697 AGCGCT
Eco81I 2179 CCTCAGG CC^T[ACGT]AGG
5372 CCTCAGG
EcoO65I 343 GGTGACC G^GT[ACGT]ACC
5419 GGTAACC
EcoRV 1575 GATATC GAT^ATC
2090 GATATC
MroI 187 TCCGGA T^CCGGA
7258 TCCGGA
PaeR7I 1469 CTCGAG C^TCGAG
4714 CTCGAG
PinAI 2002 ACCGGT A^CCGGT
5628 ACCGGT
PmaCI 3495 CACGTG CAC^GTG
4674 CACGTG
PmlI 3495 CACGTG CAC^GTG
4674 CACGTG
Psp1406I 4422 AACGTT AA^CGTT
6703 AACGTT
SgrAI 3977 CACCGGCG C[GA]^CCGG[CT]G
5627 CACCGGTG
SspI 4491 AATATT AAT^ATT
7526 AATATT
XhoI 1469 CTCGAG C^TCGAG
4714 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 156 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
ApaLI ApoI AspHI AspI AvaI
AvaII AviII BalI BanI BanII
BbeI BbsI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EagI EarI EclXI Eco52I EcoNI
EcoO109I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV NsiI NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI SphI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmaCI
XmaI