Gene/Protein Characteristic Table for KIAA0397
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Product ID ORK01082
Accession No AB007857
Description small G protein signaling modulator 2, transcript variant 2
Clone name fj00010
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (4734 bp)
Predicted protein sequence (1016 aa)
Flexi ORF Clone FXC01082
Source Human fetal brain
Rouge ID mKIAA0397 by Kazusa Mouse cDNA Project
Note We replaced hg00184, former representative clones for KIAA0397 with fj00010. (2002/5/10)
Features of the cloned cDNA sequence
Description

Length: 4734 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning Warning
Integrity of 3' end
Length of 3'UTR 1538 bp
Genome contig ID gi51511734f_2087639
PolyA signal sequence
(AATAAA,-25)
+----*----+----*----+----*----+----
GTAACAGACCAATAAACAACATTTGTCAACACTGG
Flanking genome sequence
(143465 - 143514)
----+----*----+----*----+----*----+----*----+----*
AGCCTCTGGGACTGGACACTGAGAGACCACGTGCCTTCTTTAAGCTCCAC

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 17 f 2187558 2231102 23 99.7 Perfect prediction
Features of the protein sequence
Description

Length: 1016 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
O43147 0 100.0 Small G protein...
Homo sapiens
EAW90547 0 99.8 RUN and TBC1 do...
Homo sapiens
EAW90545 0 99.6 RUN and TBC1 do...
Homo sapiens
BAG58030 0 99.9 unnamed protein...
Homo sapiens
EDM05191 0 93.0 RUN and TBC1 do...
Rattus norvegicus
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB075821 1.4e-56 50.8 KIAA1941
AB037743 0.00039 26.0 KIAA1322
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR004012 52 199 PF02759 RUN
IPR000195 768 975 PF00566 RabGAP/TBC
HMMSmart IPR004012 138 198 SM00593 RUN
IPR000195 573 976 SM00164 RabGAP/TBC
ProfileScan IPR004012 44 201 PS50826 RUN
IPR000195 576 949 PS50086 RabGAP/TBC
Expression profile
Description

RT-PCR
Experimental conditions
Primer_f TCTGCTCCTCACAAAACCACG
Primer_r ATTGTCCATCCTTGTTGTCCG
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 17
Experimental conditions
Panel name GeneBridge 4
Primer_f TCTGCTCCTCACAAAACCACG
Primer_r ATTGTCCATCCTTGTTGTCCG
PCR product length 99 bp
PCR conditions 95 °C15 sec64 °C60 sec30 cycles
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