##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AccI AclI AcsI AflII AhdI
Aor51HI ApoI AscI AseI AsnI
AspEI BciVI BfrI BglII BsaBI
BsiWI BspDI BspLU11I BssHII Bst1107I
BstXI BstZ17I ClaI CpoI DraI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV FseI HindIII HpaI
MamI MfeI MluI MunI NaeI
NdeI NgoMIV NotI NruI PacI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacI SalI SapI SexAI
SgrAI SnaBI SpeI SspI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 1980 GGTACC G^GTACC
AgeI 1614 ACCGGT A^CCGGT
Asp700I 1339 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1980 GGTACC G^GTACC
AviII 363 TGCGCA TGC^GCA
BbvCI 538 CCTCAGC CCTCAGC(-5/-2)
BcgI 2079 GCAGCAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2731 TGATCA T^GATCA
BsrDI 295 CATTGC GCAATG(2/0)
BstBI 2366 TTCGAA TT^CGAA
DraIII 3164 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1717 GACCCACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 2731 TGATCA T^GATCA
FspI 363 TGCGCA TGC^GCA
HincII 4724 GTCAAC GT[CT]^[GA]AC
HindII 4724 GTCAAC GT[CT]^[GA]AC
KpnI 1980 GGTACC GGTAC^C
KspI 1779 CCGCGG CCGC^GG
NheI 4192 GCTAGC G^CTAGC
NspV 2366 TTCGAA TT^CGAA
PaeR7I 2621 CTCGAG C^TCGAG
PinAI 1614 ACCGGT A^CCGGT
SacII 1779 CCGCGG CCGC^GG
SbfI 3353 CCTGCAGG CCTGCA^GG
ScaI 636 AGTACT AGT^ACT
SfiI 3859 GGCCTCCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 2366 TTCGAA TT^CGAA
SphI 4314 GCATGC GCATG^C
Sse8387I 3353 CCTGCAGG CCTGCA^GG
XcmI 1063 CCAGCTCATGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2621 CTCGAG C^TCGAG
XmnI 1339 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AatII 1895 GACGTC GACGT^C
2636 GACGTC
AccIII 849 TCCGGA T^CCGGA
1428 TCCGGA
Alw44I 1736 GTGCAC G^TGCAC
3654 GTGCAC
ApaLI 1736 GTGCAC G^TGCAC
3654 GTGCAC
AvrII 4233 CCTAGG C^CTAGG
4309 CCTAGG
BbeI 155 GGCGCC GGCGC^C
2704 GGCGCC
BbsI 278 GAAGAC GAAGAC(2/6)
403 GAAGAC
BlnI 4233 CCTAGG C^CTAGG
4309 CCTAGG
BpuAI 278 GAAGAC GAAGAC(2/6)
403 GAAGAC
BsaI 3718 GAGACC GGTCTC(1/5)
3733 GAGACC
BseAI 849 TCCGGA T^CCGGA
1428 TCCGGA
BsiEI 1612 CGACCG CG[GA][CT]^CG
4001 CGACCG
BsmBI 340 GAGACG CGTCTC(1/5)
2634 GAGACG
Bsp1407I 3873 TGTACA T^GTACA
3896 TGTACA
BspEI 849 TCCGGA T^CCGGA
1428 TCCGGA
BspHI 1068 TCATGA T^CATGA
2763 TCATGA
BsrFI 1614 ACCGGT [GA]^CCGG[CT]
1761 ACCGGC
BsrGI 3873 TGTACA T^GTACA
3896 TGTACA
BstEII 2716 GGTCACC G^GT[ACGT]ACC
3848 GGTGACC
BstPI 2716 GGTCACC G^GT[ACGT]ACC
3848 GGTGACC
Cfr10I 1614 ACCGGT [GA]^CCGG[CT]
1761 ACCGGC
EcoO65I 2716 GGTCACC G^GT[ACGT]ACC
3848 GGTGACC
EcoT22I 2870 ATGCAT ATGCA^T
4153 ATGCAT
KasI 155 GGCGCC G^GCGCC
2704 GGCGCC
MroI 849 TCCGGA T^CCGGA
1428 TCCGGA
NarI 155 GGCGCC GG^CGCC
2704 GGCGCC
NcoI 174 CCATGG C^CATGG
2802 CCATGG
NsiI 2870 ATGCAT ATGCA^T
4153 ATGCAT
RcaI 1068 TCATGA T^CATGA
2763 TCATGA
SfoI 155 GGCGCC GGC^GCC
2704 GGCGCC
SspBI 3873 TGTACA T^GTACA
3896 TGTACA
Tsp509I 2309 AATT ^AATT
2529 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
AspHI AspI AvaI AvaII BalI
BamHI BanI BanII BbrPI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaAI BsaHI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I TspRI Tth111I TthHB8I Van91I
XhoII XmaCI XmaI