KIAA0247

Product ID	ORK00041
Accession No	D87434
Description	sushi domain containing 6
Clone name	ha07001
Vector information	The cDNA fragment was inserted at the EcoRV-NotI site of the ... The cDNA fragment was inserted at the EcoRV-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the EcoRV (blunt-end ligation) site without using any linker or adapter. The 3' end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5338 bp) Predicted protein sequence (304 aa)
HaloTag ORF Clone	FHC00041
Flexi ORF Clone	FXC00041
Source	Myeloblast cell line (KG-1)
Rouge ID	mKIAA0247 by Kazusa Mouse cDNA Project

Length: 5338 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	4158 bp
Genome contig ID	gi51511730f_69048111
PolyA signal sequence (ATTAAA,-20)	+--------+--------+----*----+---- CTGCATTTCTAAATAATTAAAAAAAAGTTCTTATG
Flanking genome sequence (203498 - 203547)	----+--------+--------+--------+--------+----* AAGGCAATTCTGGGAGATGTGATCCTTCCTGAAAGTTTTTGCAGAAACCT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	14	f	69148111	69251607	6	99.2	Perfect prediction

Length: 304 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
XP_001109433	8.9e-121	98.3	hypothetical pr...	Macaca mulatta
AAH40401	2.9e-109	89.1	RIKEN cDNA 4933...	Mus musculus
BAE37934	1.1e-108	88.8	unnamed protein...	Mus musculus
BAE30815	2.3e-108	88.8	unnamed protein...	Mus musculus
EDL02683	4.2e-108	88.8	RIKEN cDNA 4933...	Mus musculus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR000436	43	103	PF00084	Sushi/SCR/CCP
HMMSmart	IPR000436	43	103	SM00032	Sushi/SCR/CCP
ProfileScan	IPR000436	41	105	PS50923	Sushi/SCR/CCP

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	17	VASVGHGVFLPLVILCTLLGDGL	39	SECONDARY	23
SOSUI2	2	120	LSIVASTASSVALILLLVVLFVL	142	PRIMARY	23

Primer_f
Primer_r
PCR conditions	test

Panel name	Genebridge 4
Primer_f	TAATTGACTCTGTGCAGGATG
Primer_r	TACTATCTTCACACCCACTGC
PCR product length	108 bp
PCR conditions	95 °C15 sec66 °C60 sec30 cycles