RSPRY1 ORF clone pFN21ASDA1972

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Halotag ORF Clone pFN21ASDA1972

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Clone info.

Species

human

Clone Name

pFN21ASDA1972 Sequence (with vector) Restriction Map

Product ID

FHC00963

Flexi Clone PID

FXC00963

ORF Sequence

DNA sequence (1728 bp)
Protein sequence (576 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384341]

Gene

KIAA1972

Symbol

RSPRY1

Alias : SEMDFA

Description

ring finger and SPRY domain containing 1, transcript variant 1

Original Clone ID

fk09334

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1728 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 576 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS10775.1 1.4e-201 100.0 RSPRY1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR003877 359 481 PF00622 SPla/RYanodine receptor SPRY HMMSmart IPR018355 358 482 SM00449 SPla/RYanodine receptor subgroup IPR001841 527 561 SM00184 Zinc finger ProfileScan IPR001870 300 483 PS50188 B302/SPRY domain IPR001841 527 562 PS50089 Zinc finger
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 1 MIVFGWAVFLASRSLGQGLLLTL 23 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001292092 576 616585,616723 100.0 100.0 XP_005256277 576 616585,616723 100.0 100.0 NP_588609 576 616585,616723 100.0 100.0 NP_001292093 576 616585,616723 100.0 100.0 XP_011521729 607 616585,616723 94.7 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA1972 Download

>KIAA1972  1728 bp
ATGATCGTCTTTGGTTGGGCCGTGTTCTTAGCGAGCAGAAGCCTTGGCCAGGGTCTGTTG
TTGACTCTCGAAGAGCACATAGCCCACTTCCTAGGGACTGGAGGTGCCGCTACTACCATG
GGTAATTCCTGTATCTGCCGAGATGACAGTGGAACAGATGACAGTGTTGACACCCAACAG
CAACAGGCCGAGAACAGTGCAGTACCCACTGCTGACACAAGGAGCCAACCACGGGACCCT
GTTCGGCCACCAAGGAGGGGCCGAGGACCTCATGAGCCAAGGAGAAAGAAACAAAATGTG
GATGGGCTAGTGTTGGACACACTGGCAGTAATACGGACTCTTGTAGATAATGATCAGGAA
CCTCCCTATTCAATGATAACATTACACGAAATGGCAGAAACAGATGAAGGATGGTTGGAT
GTTGTCCAGTCTTTAATTAGAGTTATTCCACTGGAAGATCCACTGGGACCAGCTGTTATA
ACATTGTTACTAGATGAATGTCCATTGCCCACTAAAGATGCACTCCAGAAATTGACTGAA
ATTCTCAATTTAAATGGAGAAGTAGCTTGCCAGGACTCAAGCCATCCTGCCAAACACAGG
AACACATCTGCAGTCCTAGGCTGCTTGGCCGAGAAACTAGCAGGTCCTGCAAGTATAGGT
TTACTTAGCCCAGGAATACTGGAATACTTGCTACAGTGTCTGAAGTTACAGTCCCACCCC
ACAGTCATGCTTTTTGCACTTATCGCACTGGAAAAGTTTGCACAGACAAGTGAAAATAAA
TTGACTATTTCTGAATCCAGTATTAGTGACCGGCTTGTCACATTGGAGTCCTGGGCTAAT
GATCCTGATTATCTGAAACGTCAAGTTGGTTTCTGTGCCCAGTGGAGCTTAGACAATCTC
TTTTTAAAAGAAGGTAGACAGCTGACCTATGAGAAAGTGAACTTGAGTAGCATTAGGGCC
ATGCTGAATAGCAATGATGTCAGCGAGTACCTGAAGATCTCACCTCATGGCTTAGAGGCT
CGCTGTGATGCCTCCTCTTTTGAAAGTGTGCGTTGCACCTTTTGTGTGGATGCCGGGGTA
TGGTACTATGAAGTAACAGTGGTCACTTCTGGCGTCATGCAGATTGGCTGGGCCACTCGA
GACAGCAAATTCCTCAATCATGAAGGCTACGGCATTGGGGATGATGAATACTCCTGTGCG
TATGATGGCTGCCGGCAGCTGATTTGGTACAATGCCAGAAGTAAGCCTCACATACACCCA
TGCTGGAAAGAAGGAGATACAGTAGGATTTCTGTTAGACTTGAATGAAAAGCAAATGATC
TTCTTTTTAAATGGCAACCAGCTGCCTCCTGAAAAGCAAGTCTTTTCATCTACTGTATCT
GGATTTTTTGCTGCAGCTAGTTTCATGTCATATCAACAATGTGAGTTCAATTTTGGAGCA
AAACCATTCAAATACCCACCATCTATGAAATTTAGCACTTTTAATGACTACGCCTTCCTA
ACAGCTGAAGAAAAAATCATTTTGCCAAGGCACAGGCGTCTTGCTCTGTTGAAGCAAGTC
AGTATCCGAGAAAACTGCTGTTCCCTTTGTTGTGATGAGGTAGCAGACACACAATTGAAG
CCATGTGGACACAGTGACCTGTGCATGGATTGTGCCTTGCAGCTGGAGACCTGCCCATTG
TGTCGTAAAGAAATAGTATCTAGAATCAGACAGATTTCTCATATTTCA

ORF Protein

Cloned ORF protein sequence for pFN21ASDA1972 Download

>KIAA1972  576 aa
MIVFGWAVFLASRSLGQGLLLTLEEHIAHFLGTGGAATTMGNSCICRDDSGTDDSVDTQQ
QQAENSAVPTADTRSQPRDPVRPPRRGRGPHEPRRKKQNVDGLVLDTLAVIRTLVDNDQE
PPYSMITLHEMAETDEGWLDVVQSLIRVIPLEDPLGPAVITLLLDECPLPTKDALQKLTE
ILNLNGEVACQDSSHPAKHRNTSAVLGCLAEKLAGPASIGLLSPGILEYLLQCLKLQSHP
TVMLFALIALEKFAQTSENKLTISESSISDRLVTLESWANDPDYLKRQVGFCAQWSLDNL
FLKEGRQLTYEKVNLSSIRAMLNSNDVSEYLKISPHGLEARCDASSFESVRCTFCVDAGV
WYYEVTVVTSGVMQIGWATRDSKFLNHEGYGIGDDEYSCAYDGCRQLIWYNARSKPHIHP
CWKEGDTVGFLLDLNEKQMIFFLNGNQLPPEKQVFSSTVSGFFAAASFMSYQQCEFNFGA
KPFKYPPSMKFSTFNDYAFLTAEEKIILPRHRRLALLKQVSIRENCCSLCCDEVADTQLK
PCGHSDLCMDCALQLETCPLCRKEIVSRIRQISHIS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA1972 Download

>pFN21ASDA1972  6457 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGATCGTCTTTGGTTG
GGCCGTGTTCTTAGCGAGCAGAAGCCTTGGCCAGGGTCTGTTGTTGACTCTCGAAGAGCA
CATAGCCCACTTCCTAGGGACTGGAGGTGCCGCTACTACCATGGGTAATTCCTGTATCTG
CCGAGATGACAGTGGAACAGATGACAGTGTTGACACCCAACAGCAACAGGCCGAGAACAG
TGCAGTACCCACTGCTGACACAAGGAGCCAACCACGGGACCCTGTTCGGCCACCAAGGAG
GGGCCGAGGACCTCATGAGCCAAGGAGAAAGAAACAAAATGTGGATGGGCTAGTGTTGGA
CACACTGGCAGTAATACGGACTCTTGTAGATAATGATCAGGAACCTCCCTATTCAATGAT
AACATTACACGAAATGGCAGAAACAGATGAAGGATGGTTGGATGTTGTCCAGTCTTTAAT
TAGAGTTATTCCACTGGAAGATCCACTGGGACCAGCTGTTATAACATTGTTACTAGATGA
ATGTCCATTGCCCACTAAAGATGCACTCCAGAAATTGACTGAAATTCTCAATTTAAATGG
AGAAGTAGCTTGCCAGGACTCAAGCCATCCTGCCAAACACAGGAACACATCTGCAGTCCT
AGGCTGCTTGGCCGAGAAACTAGCAGGTCCTGCAAGTATAGGTTTACTTAGCCCAGGAAT
ACTGGAATACTTGCTACAGTGTCTGAAGTTACAGTCCCACCCCACAGTCATGCTTTTTGC
ACTTATCGCACTGGAAAAGTTTGCACAGACAAGTGAAAATAAATTGACTATTTCTGAATC
CAGTATTAGTGACCGGCTTGTCACATTGGAGTCCTGGGCTAATGATCCTGATTATCTGAA
ACGTCAAGTTGGTTTCTGTGCCCAGTGGAGCTTAGACAATCTCTTTTTAAAAGAAGGTAG
ACAGCTGACCTATGAGAAAGTGAACTTGAGTAGCATTAGGGCCATGCTGAATAGCAATGA
TGTCAGCGAGTACCTGAAGATCTCACCTCATGGCTTAGAGGCTCGCTGTGATGCCTCCTC
TTTTGAAAGTGTGCGTTGCACCTTTTGTGTGGATGCCGGGGTATGGTACTATGAAGTAAC
AGTGGTCACTTCTGGCGTCATGCAGATTGGCTGGGCCACTCGAGACAGCAAATTCCTCAA
TCATGAAGGCTACGGCATTGGGGATGATGAATACTCCTGTGCGTATGATGGCTGCCGGCA
GCTGATTTGGTACAATGCCAGAAGTAAGCCTCACATACACCCATGCTGGAAAGAAGGAGA
TACAGTAGGATTTCTGTTAGACTTGAATGAAAAGCAAATGATCTTCTTTTTAAATGGCAA
CCAGCTGCCTCCTGAAAAGCAAGTCTTTTCATCTACTGTATCTGGATTTTTTGCTGCAGC
TAGTTTCATGTCATATCAACAATGTGAGTTCAATTTTGGAGCAAAACCATTCAAATACCC
ACCATCTATGAAATTTAGCACTTTTAATGACTACGCCTTCCTAACAGCTGAAGAAAAAAT
CATTTTGCCAAGGCACAGGCGTCTTGCTCTGTTGAAGCAAGTCAGTATCCGAGAAAACTG
CTGTTCCCTTTGTTGTGATGAGGTAGCAGACACACAATTGAAGCCATGTGGACACAGTGA
CCTGTGCATGGATTGTGCCTTGCAGCTGGAGACCTGCCCATTGTGTCGTAAAGAAATAGT
ATCTAGAATCAGACAGATTTCTCATATTTCATACGTAGTTTAAACGAATTCGGGCTCGGT
ACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAG
CCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTG
GGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACT
AGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTA
ACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAG
GTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAA
ATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAG
CTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCT
TAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTC
TAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAA
TATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTT
GCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCT
GAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATC
CTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTA
TGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACAC
TATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGC
ATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAAC
TTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGG
GATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGAC
GAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGC
GAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTT
GCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGA
GCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCC
CGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAG
ATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCG
ACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGG
ATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGG
CCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGAC
GCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTG
GAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCT
TTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGG
TGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCT
GCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCAC
TGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGT
TCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTC
TGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCA
CCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGAT
TTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCAC
GTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGT
CCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAA
TGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTA
TTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCA
TTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAG
GGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACT
CCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT