Restriction map for
pFN21ASDA1972
(Linear) (Six-base) MAPSORT of: KIAA1972.seq Check: 1842 from: 1 to: 1728
KIAA1972.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AccI GT'mk_AC
Cuts at: 0 915 1728
Size: 915 813
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 721 1012 1526 1728
Size: 721 291 514 202
Fragments arranged by size:
721 514 291 202
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1357 1728
Size: 1357 371
AlwNI CAG_nnn'CTG
Cuts at: 0 54 646 699 1728
Size: 54 592 53 1029
Fragments arranged by size:
1029 592 54 53
ApoI r'AATT_y
Cuts at: 0 539 1147 1468 1728
Size: 539 608 321 260
Fragments arranged by size:
608 539 321 260
AvaI C'yCGr_G
Cuts at: 0 1136 1728
Size: 1136 592
AvrII C'CTAG_G
Cuts at: 0 90 615 1728
Size: 90 525 1113
Fragments arranged by size:
1113 525 90
BanI G'GyrC_C
Cuts at: 0 103 1728
Size: 103 1625
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 178 212 1556 1590 1728
Size: 178 34 1344 34 138
Fragments arranged by size:
1344 178 138 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1573 1728
Size: 1573 155
BclI T'GATC_A
Cuts at: 0 351 1728
Size: 351 1377
BglII A'GATC_T
Cuts at: 0 995 1728
Size: 995 733
Bme1580I G_kGCm'C
Cuts at: 0 879 1728
Size: 879 849
BmrI ACTGGGnnnn_n'
Cuts at: 0 472 873 1728
Size: 472 401 855
Fragments arranged by size:
855 472 401
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 508 1684 1728
Size: 119 389 1176 44
Fragments arranged by size:
1176 389 119 44
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 561 963 1728
Size: 561 402 765
Fragments arranged by size:
765 561 402
BsaI GGTCTCn'nnnn_
Cuts at: 0 1660 1728
Size: 1660 68
BsaBI GATnn'nnATC
Cuts at: 0 350 1728
Size: 350 1378
BsaHI Gr'CG_yC
Cuts at: 0 1112 1536 1728
Size: 1112 424 192
Fragments arranged by size:
1112 424 192
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1265 1295 1728
Size: 1265 30 433
Fragments arranged by size:
1265 433 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1023 1728
Size: 1023 705
BseYI C'CCAG_C
Cuts at: 0 1127 1728
Size: 1127 601
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 1728
Size: 218 1510
BsiHKAI G_wGCw'C
Cuts at: 0 77 1728
Size: 77 1651
Bsp1286I G_dGCh'C
Cuts at: 0 77 879 1728
Size: 77 802 849
Fragments arranged by size:
849 802 77
BspHI T'CATG_A
Cuts at: 0 270 1158 1728
Size: 270 888 570
Fragments arranged by size:
888 570 270
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 631 1678 1728
Size: 631 1047 50
Fragments arranged by size:
1047 631 50
BsrDI GCAATG_nn'
Cuts at: 0 502 978 1728
Size: 502 476 750
Fragments arranged by size:
750 502 476
BsrFI r'CCGG_y
Cuts at: 0 809 1211 1728
Size: 809 402 517
Fragments arranged by size:
809 517 402
BstAPI GCAn_nnn'nTGC
Cuts at: 0 646 1728
Size: 646 1082
BstYI r'GATC_y
Cuts at: 0 456 995 1728
Size: 456 539 733
Fragments arranged by size:
733 539 456
BtgI C'CryG_G
Cuts at: 0 116 229 1728
Size: 116 113 1499
Fragments arranged by size:
1499 116 113
BtsI GCAGTG_nn'
Cuts at: 0 206 1728
Size: 206 1522
DraI TTT'AAA
Cuts at: 0 551 905 1328 1728
Size: 551 354 423 400
Fragments arranged by size:
551 423 400 354
DrdI GACnn_nn'nnGTC
Cuts at: 0 814 1728
Size: 814 914
EaeI y'GGCC_r
Cuts at: 0 45 244 626 1728
Size: 45 199 382 1102
Fragments arranged by size:
1102 382 199 45
EarI CTCTTCn'nnn_
Cuts at: 0 65 1728
Size: 65 1663
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 508 721 1012 1526 1684 1728
Size: 119 389 213 291 514 158 44
Fragments arranged by size:
514 389 291 213 158 119 44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 46 1728
Size: 46 1682
EcoO109I rG'GnC_Cy
Cuts at: 0 234 265 643 1728
Size: 234 31 378 1085
Fragments arranged by size:
1085 378 234 31
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1377 1728
Size: 1377 351
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 987 1018 1019 1050 1174 1206 1728
Size: 987 31 1 31 124 32 522
Fragments arranged by size:
987 522 124 32 31 31 1
HincII GTy'rAC
Cuts at: 0 62 168 1728
Size: 62 106 1560
Fragments arranged by size:
1560 106 62
MfeI C'AATT_G
Cuts at: 0 1612 1728
Size: 1612 116
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 395 1728
Size: 293 102 1333
Fragments arranged by size:
1333 293 102
MscI TGG'CCA
Cuts at: 0 47 1728
Size: 47 1681
MslI CAynn'nnrTG
Cuts at: 0 724 1728
Size: 724 1004
MspA1I CmG'CkG
Cuts at: 0 472 921 1218 1341 1504 1662 1728
Size: 472 449 297 123 163 158 66
Fragments arranged by size:
472 449 297 163 158 123 66
NaeI GCC'GGC
Cuts at: 0 1213 1728
Size: 1213 515
NcoI C'CATG_G
Cuts at: 0 116 1728
Size: 116 1612
NgoMIV G'CCGG_C
Cuts at: 0 1211 1728
Size: 1211 517
PpuMI rG'GwC_Cy
Cuts at: 0 234 265 643 1728
Size: 234 31 378 1085
Fragments arranged by size:
1085 378 234 31
PsiI TTA'TAA
Cuts at: 0 478 1728
Size: 478 1250
PstI C_TGCA'G
Cuts at: 0 612 1395 1728
Size: 612 783 333
Fragments arranged by size:
783 612 333
PvuII CAG'CTG
Cuts at: 0 472 921 1218 1341 1504 1662 1728
Size: 472 449 297 123 163 158 66
Fragments arranged by size:
472 449 297 163 158 123 66
SanDI GG'GwC_CC
Cuts at: 0 234 1728
Size: 234 1494
SapI GCTCTTCn'nnn_
Cuts at: 0 65 1728
Size: 65 1663
SfcI C'TryA_G
Cuts at: 0 608 691 1391 1728
Size: 608 83 700 337
Fragments arranged by size:
700 608 337 83
SmlI C'TyrA_G
Cuts at: 0 576 942 1136 1728
Size: 576 366 194 592
Fragments arranged by size:
592 576 366 194
StyI C'CwwG_G
Cuts at: 0 42 90 116 250 277 615 1525 1728
Size: 42 48 26 134 27 338 910 203
Fragments arranged by size:
910 338 203 134 48 42 27 26
SwaI ATTT'AAAT
Cuts at: 0 551 1728
Size: 551 1177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 187 1271 1728
Size: 187 1084 457
Fragments arranged by size:
1084 457 187
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 87 119 1728
Size: 87 32 1609
Fragments arranged by size:
1609 87 32
XbaI T'CTAG_A
Cuts at: 0 1699 1728
Size: 1699 29
XhoI C'TCGA_G
Cuts at: 0 1136 1728
Size: 1136 592
Enzymes that do cut:
AccI AcuI AlfI AlwNI ApoI AvaI AvrII BanI
BcgI BciVI BclI BglII Bme1580I BmrI BpmI BpuEI
BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspHI BspMI BsrDI BsrFI BstAPI BstYI BtgI
BtsI DraI DrdI EaeI EarI Eco57MI EcoNI EcoO109I
FalI Hin4I HincII MfeI MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV PpuMI PsiI PstI PvuII SanDI
SapI SfcI SmlI StyI SwaI TaqII TstI XbaI
XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
BaeI BamHI BanII BbeI BbsI BbvCI BfrBI BglI
BlpI BmgBI BmtI BplI Bpu10I BsaAI BsaWI BsiEI
BsiWI BsmI BsmBI BspEI BsrBI BsrGI BssHII BssSI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI
DraIII EagI EciI EcoICRI EcoRI EcoRV FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MluI NarI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI TatI Tth111I XcmI
XmaI XmnI ZraI