Restriction map for
pFN21ASDA1972
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII BamHI BanII BbeI
BbrPI BbsI BbvCI BfrI BglI
BlpI Bpu1102I BpuAI BsaAI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI HaeII
HhaI HinP1I HindIII HpaI KasI
KpnI KspI MluI MroI MvnI
NarI NdeI NheI NotI NruI
NsiI NspI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI Tth111I Van91I
XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 914 GTAGAC GT^[AC][GT]AC
AciI 107 CCGC CCGC(-3/-1)
AspHI 73 GAGCAC G[AT]GC[AT]^C
AvaI 1136 CTCGAG C^[CT]CG[GA]G
BalI 45 TGGCCA TGG^CCA
BanI 103 GGTGCC G^G[CT][GA]CC
BciVI 1562 GTATCC GTATCC(6/5)
BclI 351 TGATCA T^GATCA
BcnI 1073 CCGGG CC^[GC]GG
BglII 995 AGATCT A^GATCT
BsaBI 346 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1666 GAGACC GGTCTC(1/5)
BseRI 1032 CTCCTC GAGGAG(10/8)
BsgI 197 GTGCAG GTGCAG(16/14)
BsiHKAI 73 GAGCAC G[AT]GC[AT]^C
BsoBI 1136 CTCGAG C^[CT]CG[GA]G
BstAPI 640 GCAGGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI 808 GACCGGCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 70 GAAGAG CTCTTC(1/4)
EcoNI 42 CCTTGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 351 TGATCA T^GATCA
HphI 1000 TCACC GGTGA(8/7)
Ksp632I 70 GAAGAG CTCTTC(1/4)
MaeII 858 ACGT A^CGT
MamI 346 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1612 CAATTG C^AATTG
MluNI 45 TGGCCA TGG^CCA
MscI 45 TGGCCA TGG^CCA
MslI 720 CACAGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1612 CAATTG C^AATTG
NaeI 1211 GCCGGC GCC^GGC
NciI 1073 CCGGG CC^[GC]GG
NcoI 116 CCATGG C^CATGG
NgoMIV 1211 GCCGGC G^CCGGC
PaeR7I 1136 CTCGAG C^TCGAG
SapI 70 GAAGAGC GCTCTTC(1/4)
SwaI 548 ATTTAAAT ATTT^AAAT
XbaI 1699 TCTAGA T^CTAGA
XhoI 1136 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 1111 GGCGTC G[GA]^CG[CT]C
1535 GGCGTC
AlwI 457 GATCC GGATC(4/5)
841 GATCC
AvrII 90 CCTAGG C^CTAGG
615 CCTAGG
BcgI 189 CGAGAACAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1567 CGAGAAAACTGC
BlnI 90 CCTAGG C^CTAGG
615 CCTAGG
BmyI 73 GAGCAC G[AGT]GC[ACT]^C
875 GTGCCC
BsaHI 1111 GGCGTC G[GA]^CG[CT]C
1535 GGCGTC
BsmAI 1139 GAGAC GTCTC(1/5)
1666 GAGAC
Bsp1286I 73 GAGCAC G[AGT]GC[ACT]^C
875 GTGCCC
BspHI 270 TCATGA T^CATGA
1158 TCATGA
BspMI 1669 ACCTGC ACCTGC(4/8)
640 GCAGGT
BsrDI 503 CATTGC GCAATG(2/0)
971 GCAATG
BsrFI 809 ACCGGC [GA]^CCGG[CT]
1211 GCCGGC
BstYI 456 AGATCC [GA]^GATC[CT]
995 AGATCT
Cfr10I 809 ACCGGC [GA]^CCGG[CT]
1211 GCCGGC
DsaI 116 CCATGG C^C[GA][CT]GG
229 CCACGG
HgaI 1112 GCGTC GACGC(5/10)
1536 GCGTC
Hin1I 1111 GGCGTC G[GA]^CG[CT]C
1535 GGCGTC
HincII 60 GTTGAC GT[CT]^[GA]AC
166 GTTGAC
HindII 60 GTTGAC GT[CT]^[GA]AC
166 GTTGAC
MflI 456 AGATCC [GA]^GATC[CT]
995 AGATCT
MwoI 640 GCAGGTCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
736 GCACTTATCGC
PstI 608 CTGCAG CTGCA^G
1391 CTGCAG
RcaI 270 TCATGA T^CATGA
1158 TCATGA
TaqI 68 TCGA T^CGA
1137 TCGA
TfiI 793 GAATC G^A[AT]TC
1703 GAATC
TthHB8I 68 TCGA T^CGA
1137 TCGA
XhoII 456 AGATCC [GA]^GATC[CT]
995 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AcsI AfaI AluI AlwNI ApoI
AvaII BbvI BfaI BpmI BsaJI
BsiYI BslI BsmFI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraI DraII EaeI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII ItaI MaeI
MaeIII MboI MboII MnlI MseI
MspA1I MspI MvaI NdeII NlaIII
NlaIV PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI Tru9I TseI Tsp45I
Tsp509I TspRI