(Circular) (Six-base) MAPSORT of: pFN21ASDA1972.seq Check: 7752 from: 1 to: 6457 pFN21ASDA1972 6457 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4267 278 Size: 53 83 186 936 2731 2468 Fragments arranged by size: 2731 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2938 3800 1937 Size: 1001 862 4594 Fragments arranged by size: 4594 1001 862 Acc65I G'GTAC_C Cuts at: 3778 3778 Size: 6457 AclI AA'CG_TT Cuts at: 1563 4584 4957 1563 Size: 3021 373 3063 Fragments arranged by size: 3063 3021 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2744 3035 3549 4520 5904 6172 1632 Size: 1112 291 514 971 1384 268 1917 Fragments arranged by size: 1917 1384 1112 971 514 291 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6260 AflIII A'CryG_T Cuts at: 1203 5356 6333 1203 Size: 4153 977 1327 Fragments arranged by size: 4153 1327 977 AgeI A'CCGG_T Cuts at: 5288 5288 Size: 6457 AhdI GACnn_n'nnGTC Cuts at: 5186 5186 Size: 6457 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6457 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3380 1184 Size: 2196 4261 AlwNI CAG_nnn'CTG Cuts at: 2077 2669 2722 4200 5772 2077 Size: 592 53 1478 1572 2762 Fragments arranged by size: 2762 1572 1478 592 53 ApaLI G'TGCA_C Cuts at: 4514 5670 4514 Size: 1156 5301 ApoI r'AATT_y Cuts at: 1493 2562 3170 3491 3766 3992 4144 6253 1493 Size: 1069 608 321 275 226 152 2109 1697 Fragments arranged by size: 2109 1697 1069 608 321 275 226 152 AseI AT'TA_AT Cuts at: 160 1032 5011 160 Size: 872 3979 1606 Fragments arranged by size: 3979 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6457 AvaI C'yCGr_G Cuts at: 1870 1943 3159 3782 1870 Size: 73 1216 623 4545 Fragments arranged by size: 4545 1216 623 73 AvrII C'CTAG_G Cuts at: 2113 2638 2113 Size: 525 5932 BamHI G'GATC_C Cuts at: 3787 4154 3787 Size: 367 6090 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2126 3778 5233 618 Size: 325 217 627 339 1652 1455 1842 Fragments arranged by size: 1842 1652 1455 627 339 325 217 BanII G_rGCy'C Cuts at: 1391 3776 1391 Size: 2385 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5486 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2201 2235 3579 3613 4648 4682 1903 Size: 34 264 34 1344 34 1035 34 3678 Fragments arranged by size: 3678 1344 1035 264 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3596 4349 5559 3596 Size: 753 1210 4494 Fragments arranged by size: 4494 1210 753 BclI T'GATC_A Cuts at: 1548 2374 1548 Size: 826 5631 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5068 136 Size: 107 122 71 631 605 3396 1525 Fragments arranged by size: 3396 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3018 6452 3018 Size: 3434 3023 BlpI GC'TnA_GC Cuts at: 3874 3874 Size: 6457 Bme1580I G_kGCm'C Cuts at: 1790 2902 4518 5674 1790 Size: 1112 1616 1156 2573 Fragments arranged by size: 2573 1616 1156 1112 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6457 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2495 2896 4188 Size: 416 53 83 399 24 1078 401 1292 Cuts at: 4188 5146 442 Size: 958 1753 Fragments arranged by size: 1753 1292 1078 958 416 401 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6457 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2142 2531 3707 5117 1239 Size: 903 389 1176 1410 2579 Fragments arranged by size: 2579 1410 1176 903 389 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2584 2986 4582 5447 5745 2584 Size: 402 1596 865 298 3296 Fragments arranged by size: 3296 1596 865 402 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3683 5120 882 Size: 617 2184 1437 2219 Fragments arranged by size: 2219 2184 1437 617 BsaAI yAC'GTr Cuts at: 493 1204 3754 4248 493 Size: 711 2550 494 2702 Fragments arranged by size: 2702 2550 711 494 BsaBI GATnn'nnATC Cuts at: 2373 6451 2373 Size: 4078 2379 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3135 3559 4264 4646 Size: 53 83 186 936 1602 424 705 382 Cuts at: 4646 5281 275 Size: 635 1451 Fragments arranged by size: 1602 1451 936 705 635 424 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4890 5288 5562 5709 6120 4890 Size: 398 274 147 411 5227 Fragments arranged by size: 5227 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3288 3318 1178 Size: 30 165 30 1885 30 4317 Fragments arranged by size: 4317 1885 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3046 1186 Size: 1860 4597 BseYI C'CCAG_C Cuts at: 3150 5660 6156 3150 Size: 2510 496 3451 Fragments arranged by size: 3451 2510 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2241 1163 Size: 573 505 5379 Fragments arranged by size: 5379 573 505 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3907 4668 5696 1540 Size: 275 186 1906 761 1028 2301 Fragments arranged by size: 2301 1906 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2100 4518 4603 5674 2100 Size: 2418 85 1071 2883 Fragments arranged by size: 2883 2418 1071 85 BsmI GAATG_Cn' Cuts at: 3968 4061 3968 Size: 93 6364 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2100 2902 3776 4518 4603 5674 1391 Size: 399 310 802 874 742 85 1071 2174 Fragments arranged by size: 2174 1071 874 802 742 399 310 85 BspEI T'CCGG_A Cuts at: 6120 6120 Size: 6457 BspHI T'CATG_A Cuts at: 2293 3181 4346 6076 2293 Size: 888 1165 1730 2674 Fragments arranged by size: 2674 1730 1165 888 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2654 3701 3812 844 Size: 1810 1047 111 3489 Fragments arranged by size: 3489 1810 1047 111 BsrBI CCG'CTC Cuts at: 1394 1823 4344 1394 Size: 429 2521 3507 Fragments arranged by size: 3507 2521 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2525 3001 4952 5126 65 Size: 1183 1277 476 1951 174 1396 Fragments arranged by size: 1951 1396 1277 1183 476 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2832 3234 5101 5288 1700 Size: 110 1022 402 1867 187 2869 Fragments arranged by size: 2869 1867 1022 402 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6457 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6457 BssSI C'ACGA_G Cuts at: 4517 5529 4517 Size: 1012 5445 BstAPI GCAn_nnn'nTGC Cuts at: 794 2669 794 Size: 1875 4582 BstBI TT'CG_AA Cuts at: 2007 5261 2007 Size: 3254 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6457 BstYI r'GATC_y Cuts at: 1972 2479 3018 3787 4154 4539 4556 6008 6094 Size: 507 539 769 367 385 17 1452 86 Cuts at: 6094 6106 6452 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 769 539 507 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2139 2252 513 Size: 552 1074 113 4718 Fragments arranged by size: 4718 1074 552 113 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5718 Fragments arranged by size: 5718 683 56 BtsI GCAGTG_nn' Cuts at: 2229 3973 4767 4787 2229 Size: 1744 794 20 3899 Fragments arranged by size: 3899 1744 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6457 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6422 DraI TTT'AAA Cuts at: 2574 2928 3351 3761 2574 Size: 354 423 410 5270 Fragments arranged by size: 5270 423 410 354 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2837 5464 809 Size: 507 1521 2627 1802 Fragments arranged by size: 2627 1802 1521 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2068 2267 2649 Size: 54 1425 50 275 18 238 199 382 Cuts at: 2649 3904 4793 6422 8 Size: 1255 889 1629 43 Fragments arranged by size: 1629 1425 1255 889 382 275 238 199 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3904 1537 Size: 275 2092 4090 Fragments arranged by size: 4090 2092 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2088 4387 1353 Size: 735 2299 3423 Fragments arranged by size: 3423 2299 735 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5044 5418 5564 387 Size: 1064 138 3455 374 146 1280 Fragments arranged by size: 3455 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2142 2531 2744 3035 3549 3707 4520 Size: 393 510 389 213 291 514 158 813 Cuts at: 4520 5117 5904 6172 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 813 787 597 514 510 393 389 291 268 213 158 EcoNI CCTnn'n_nnAGG Cuts at: 2069 2069 Size: 6457 EcoO109I rG'GnC_Cy Cuts at: 2257 2288 2666 2257 Size: 31 378 6048 Fragments arranged by size: 6048 378 31 EcoRI G'AATT_C Cuts at: 3766 4144 3766 Size: 378 6079 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3400 3400 Size: 6457 FspI TGC'GCA Cuts at: 4963 4963 Size: 6457 HaeII r_GCGC'y Cuts at: 1678 5604 1678 Size: 3926 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3010 3041 3042 3073 3197 Size: 32 135 32 1438 31 1 31 124 Cuts at: 3197 3229 5098 5130 5172 5204 1373 Size: 32 1869 32 42 32 2626 Fragments arranged by size: 2626 1869 1438 135 124 42 32 32 32 32 32 31 31 1 HincII GTy'rAC Cuts at: 669 1657 1938 2085 2191 3801 4047 669 Size: 988 281 147 106 1610 246 3079 Fragments arranged by size: 3079 1610 988 281 246 147 106 HindIII A'AGCT_T Cuts at: 748 748 Size: 6457 HpaI GTT'AAC Cuts at: 4047 4047 Size: 6457 KpnI G_GTAC'C Cuts at: 3782 3782 Size: 6457 MfeI C'AATT_G Cuts at: 3635 4056 3635 Size: 421 6036 MluI A'CGCG_T Cuts at: 6333 6333 Size: 6457 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2316 2418 5571 5755 1305 Size: 1011 102 3153 184 2007 Fragments arranged by size: 3153 2007 1011 184 102 MscI TGG'CCA Cuts at: 10 64 1489 1832 2070 10 Size: 54 1425 343 238 4397 Fragments arranged by size: 4397 1425 343 238 54 MslI CAynn'nnrTG Cuts at: 518 1166 2747 4415 4774 4933 518 Size: 648 1581 1668 359 159 2042 Fragments arranged by size: 2042 1668 1581 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2495 2944 3241 3364 3527 3685 3873 4550 Size: 889 449 297 123 163 158 188 677 Cuts at: 4550 5698 5943 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 889 677 449 297 245 188 163 158 123 NaeI GCC'GGC Cuts at: 3236 3236 Size: 6457 NcoI C'CATG_G Cuts at: 513 1065 2139 513 Size: 552 1074 4831 Fragments arranged by size: 4831 1074 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6457 NgoMIV G'CCGG_C Cuts at: 3234 3234 Size: 6457 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6457 NotI GC'GGCC_GC Cuts at: 3904 3904 Size: 6457 NspI r_CATG'y Cuts at: 1132 1229 3815 5360 1132 Size: 97 2586 1545 2229 Fragments arranged by size: 2586 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6457 PciI A'CATG_T Cuts at: 5356 5356 Size: 6457 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6457 PmeI GTTT'AAAC Cuts at: 3761 3761 Size: 6457 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4526 4558 6039 6071 4526 Size: 32 1481 32 4912 Fragments arranged by size: 4912 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2257 2288 2666 2257 Size: 31 378 6048 Fragments arranged by size: 6048 378 31 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6457 PsiI TTA'TAA Cuts at: 2501 4027 2501 Size: 1526 4931 PstI C_TGCA'G Cuts at: 830 2635 3418 3809 830 Size: 1805 783 391 3478 Fragments arranged by size: 3478 1805 783 391 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6457 PvuII CAG'CTG Cuts at: 2495 2944 3241 3364 3527 3685 2495 Size: 449 297 123 163 158 5267 Fragments arranged by size: 5267 449 297 163 158 123 SalI G'TCGA_C Cuts at: 1936 3799 1936 Size: 1863 4594 SanDI GG'GwC_CC Cuts at: 2257 2257 Size: 6457 SapI GCTCTTCn'nnn_ Cuts at: 2088 2088 Size: 6457 SbfI CC_TGCA'GG Cuts at: 3809 3809 Size: 6457 ScaI AGT'ACT Cuts at: 4705 6417 4705 Size: 1712 4745 SfcI C'TryA_G Cuts at: 826 1045 2631 2714 3414 3805 4940 5621 5812 Size: 219 1586 83 700 391 1135 681 191 Cuts at: 5812 826 Size: 1471 Fragments arranged by size: 1586 1471 1135 700 681 391 219 191 83 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6457 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6457 SmaI CCC'GGG Cuts at: 1872 3784 1872 Size: 1912 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2599 2965 3159 4561 5462 5724 Size: 197 926 656 366 194 1402 901 262 Cuts at: 5724 820 Size: 1553 Fragments arranged by size: 1553 1402 926 901 656 366 262 197 194 SnaBI TAC'GTA Cuts at: 493 3754 4248 493 Size: 3261 494 2702 Fragments arranged by size: 3261 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6457 SphI G_CATG'C Cuts at: 1132 3815 1132 Size: 2683 3774 SspI AAT'ATT Cuts at: 5 52 4381 5 Size: 47 4329 2081 Fragments arranged by size: 4329 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6457 StyI C'CwwG_G Cuts at: 513 1065 2065 2113 2139 2273 2300 2638 3548 Size: 552 1000 48 26 134 27 338 910 Cuts at: 3548 3896 513 Size: 348 3074 Fragments arranged by size: 3074 1000 910 552 348 338 134 48 27 26 SwaI ATTT'AAAT Cuts at: 2574 2574 Size: 6457 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2210 3294 4484 4501 4654 4839 5284 Size: 1255 630 1084 1190 17 153 185 445 Cuts at: 5284 325 Size: 1498 Fragments arranged by size: 1498 1255 1190 1084 630 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4703 6415 96 Size: 274 80 33 51 1443 2726 1712 138 Fragments arranged by size: 2726 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2110 2142 6417 6449 2110 Size: 32 4275 32 2118 Fragments arranged by size: 4275 2118 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6457 XbaI T'CTAG_A Cuts at: 3722 3793 3722 Size: 71 6386 XhoI C'TCGA_G Cuts at: 1943 3159 1943 Size: 1216 5241 XmaI C'CCGG_G Cuts at: 1870 3782 1870 Size: 1912 4545 XmnI GAAnn'nnTTC Cuts at: 4586 4586 Size: 6457 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4265 276 Size: 53 83 186 936 2731 2468 Fragments arranged by size: 2731 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI XcmI