NISCH ORF clone pFN21ASDA0975

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21ASDA0975

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Clone info.

Species

human

Clone Name

pFN21ASDA0975 Sequence (with vector) Restriction Map

Product ID

FHC01618

Flexi Clone PID

FXC01618

ORF Sequence

DNA sequence (4512 bp)
Protein sequence (1504 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384069]

Gene

KIAA0975

Symbol

NISCH

Alias : I-1, IR1, IRAS, hIRAS

Description

nischarin, transcript variant 1

Original Clone ID

hj06890

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 4512 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 1504 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS33767.1 0 99.8 NISCH CCDS63651.1 8.4e-91 99.6 NISCH CCDS63652.1 1.6e-90 99.8 NISCH The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001683 20 117 PF00787 Phox homologous domain IPR001611 289 305 PF00560 Leucine-rich repeat IPR001611 333 348 PF00560 Leucine-rich repeat HMMSmart IPR001683 14 118 SM00312 Phox homologous domain NULL 286 304 SM00365 NULL IPR003591 286 308 SM00369 Leucine-rich repeat NULL 309 330 SM00365 NULL NULL 331 352 SM00365 NULL IPR003591 331 353 SM00369 Leucine-rich repeat NULL 354 375 SM00365 NULL IPR003591 354 375 SM00369 Leucine-rich repeat NULL 376 397 SM00365 NULL IPR003591 376 401 SM00369 Leucine-rich repeat ProfileScan IPR001683 11 121 PS50195 Phox homologous domain IPR001611 288 309 PS51450 Leucine-rich repeat IPR001611 311 332 PS51450 Leucine-rich repeat IPR001611 333 354 PS51450 Leucine-rich repeat IPR001611 356 377 PS51450 Leucine-rich repeat IPR001611 378 399 PS51450 Leucine-rich repeat IPR001611 403 424 PS51450 Leucine-rich repeat
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_009115 1504 615507 99.9 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA0975 Download

>KIAA0975  4512 bp
ATGGCGACCGCGCGCACCTTCGGGCCCGAGCGGGAAGCCGAGCCGGCCAAGGAAGCGCGC
GTCGTGGGCTCGGAGCTTGTGGACACTTATACGGTTTACATCATCCAGGTCACTGATGGC
AGCCATGAGTGGACAGTAAAGCACCGCTACAGCGACTTCCATGACCTGCATGAAAAGCTC
GTTGCAGAGAGAAAGATTGATAAAAACCTGCTTCCGCCCAAAAAGATAATTGGGAAAAAC
TCAAGAAGCTTGGTGGAGAAGAGGGAGAAGGATCTGGAGGTCTACCTCCAGAAGCTCCTG
GCTGCCTTCCCTGGCGTGACCCCCAGAGTACTGGCCCACTTCTTGCATTTTCACTTCTAT
GAGATAAATGGCATCACCGCGGCACTGGCTGAAGAGCTCTTTGAGAAAGGAGAACAGCTC
CTGGGGGCCGGCGAGGTCTTTGCCATTGGACCCCTGCAGCTGTATGCCGTCACGGAGCAG
CTGCAGCAGGGAAAGCCCACGTGCGCCAGTGGGGATGCCAAGACCGACCTCGGGCACATC
CTGGACTTCACCTGTCGCCTTAAGTACCTTAAGGTTTCTGGCACAGAAGGACCTTTTGGG
ACCAGCAACATTCAGGAGCAGCTCCTGCCGTTCGACCTATCAATATTCAAGTCCCTGCAT
CAGGTGGAGATAAGTCACTGTGATGCTAAGCACATCAGAGGGCTGGTCGCATCGAAGCCC
ACCTTAGCCACGCTGAGTGTCCGCTTCTCAGCAACCTCGATGAAGGAAGTCCTTGTTCCT
GAAGCCTCAGAATTTGATGAGTGGGAGCCTGAAGGCACAACCCTAGAAGGCCCTGTGACT
GCCGTCATCCCCACTTGGCAGGCATTGACCACGCTTGACCTGAGCCACAACAGCATCTCC
GAGATCGACGAGTCTGTGAAACTGATCCCAAAGATTGAGTTCCTGGACCTGAGTCACAAT
GGATTGCTGGTTGTGGACAATCTGCAGCACCTGTATAACCTTGTGCATCTGGACCTGTCC
TACAACAAGCTCTCCTCCTTGGAAGGGCTTCACACCAAGCTGGGGAACATCAAGACCTTA
AACCTGGCAGGCAACCTCCTAGAGAGTCTGAGTGGCCTGCACAAGCTCTACTCACTGGTC
AACCTGGATCTCCGGGACAACAGGATCGAACAGATGGAGGAGGTCCGGAGCATAGGCAGC
CTCCCGTGTCTGGAGCACGTGTCTCTGCTGAACAACCCTCTGAGCATCATCCCCGACTAC
CGGACCAAGGTGCTGGCTCAGTTCGGAGAGAGGGCCTCAGAGGTCTGTCTGGATGACACA
GTGACCACAGAGAAGGAGCTGGACACTGTGGAAGTGCTGAAAGCAATTCAGAAAGCCAAG
GAGGTCAAGTCCAAACTGAGCAACCCAGAGAAGAAGGGTGGTGAAGACTCCCGGCTCTCA
GCTGCCCCCTGCATCAGACCCAGCAGCTCCCCTCCCACTGTGGCTCCCGCATCTGCCTCC
CTGCCCCAGCCCATCCTCTCTAACCAAGGAATCATGTTCGTTCAGGAGGAGGCCCTGGCC
AGCAGCCTCTCGTCCACTGACAGTCTGACTCCCGAGCACCAGCCCATTGCCCAGGGATGT
TCTGATTCCTTGGAGTCCATCCCTGCGGGACAGGCAGCTTCCGATGATTTAAGGGACGTG
CCAGGAGCTGTTGGTGGTGCAAGCCCAGAACATGCCGAGCCGGAGGTCCAGGTGGTGCCG
GGGTCTGGCCAGATCATCTTCCTGCCCTTCACCTGCATTGGCTACACGGCCACCAATCAG
GACTTCATCCAGCGCCTGAGCACACTGATCCGGCAGGCCATCGAGCGGCAGCTGCCTGCC
TGGATCGAGGCTGCCAACCAGCGGGAGGAGGGCCAGGGTGAACAGGGCGAGGAGGAGGAT
GAGGAGGAGGAAGAAGAGGAGGACGTGGCTGAGAACCGCTACTTTGAAATGGGGCCCCCA
GACGTGGAGGAGGAGGAGGGAGGAGGCCAGGGGGAGGAAGAGGAGGAGGAAGAGGAGGAT
GAAGAGGCCGAGGAGGAGCGCCTGGCTCTGGAATGGGCCCTGGGCGCGGACGAGGACTTC
CTGCTGGAGCACATCCGCATCCTCAAGGTGCTGTGGTGCTTCCTGATCCATGTGCAGGGC
AGTATCCGCCAGTTCGCCGCCTGCCTTGTGCTCACCGACTTCGGCATCGCAGTCTTCGAG
ATCCCGCACCAGGAGTCTCGGGGCAGCAGCCAGCACATCCTCTCCTCCCTGCGCTTTGTC
TTTTGCTTCCCGCATGGCGACCTCACCGAGTTTGGCTTCCTCATGCCGGAGCTGTGTCTG
GTGCTCAAGGTACGGCACAGTGAGAACACGCTCTTCATTATCTCGGACGCCGCCAACCTG
CACGAGTTCCACGCGGACCTGCGCTCATGCTTTGCACCCCAGCACATGGCCATGCTGTGT
AGCCCCATCCTCTACGGCAGCCACACCAGCCTGCAGGAGTTCCTGCGCCAGCTGCTCACC
TTCTACAAGGTGGCTGGCGGCTGCCAGGAGCGCAGCCAGGGCTGCTTCCCCGTCTACCTG
GTCTACAGTGACAAGCGCATGGTGCAGACGGCCGCCGGGGACTACTCAGGCAACATCGAG
TGGGCCAGCTGCACACTCTGTTCAGCCGTGCGGCGCTCCTGCTGCGCGCCCTCTGAGGCC
GTCAAGTCCGCCGCCATCCCCTACTGGCTGTTGCTCACGCCCCAGCACCTCAACGTCATC
AAGGCCGACTTCAACCCCATGCCCAACCGTGGCACCCACAACTGTCGCAACCGCAACAGC
TTCAAGCTCAGCCGTGTGCCGCTCTCCACCGTGCTGCTGGACCCCACACGCAGCTGTACC
CAGCCTCGGGGCGCCTTTGCTGATGGCCACGTGCTAGAGCTGCTCGTGGGGTACCGCTTT
GTCACTGCCATCTTCGTGCTGCCCCACGAGAAGTTCCACTTCCTGCGCGTCTACAACCAG
CTGCGGGCCTCGCTGCAGGACCTGAAGACTGTGGTCATCGCCAAGACCCCCGGGACGGGA
GGCAGCGCCCAGGGCTCCTTTGCGGATGGCCAGCCTGCCGAGCGCAGGGCCAGCAATGAC
CAGCGTCCCCAGGAGGTCCCAGCAGAGGCTCTGGCCCCGGCCCCAGTGGAAGTCCCAGCT
CCAGCCCCTGCAGCAGCCTCAGCCTCAGGCCCAGCGAAGACTCCGGCCCCAGCAGAGGCC
TCAACTTCAGCTTTGGTCCCAGAGGAGACGCCAGTGGAAGCTCCAGCCCCACCCCCAGCC
GAGGCCCCTGCCCAGTACCCGAGTGAGCACCTCATCCAGGCCACCTCGGAGGAGAATCAG
ATCCCCTCGCACTTGCCTGCCTGCCCGTCGCTCCGGCACGTCGCCAGCCTGCGGGGCAGC
GCCATCATCGAGCTCTTCCACAGCAGCATTGCTGAGGTTGAAAACGAGGAGCTGAGGCAC
CTCATGTGGTCCTCGGTGGTGTTCTACCAGACCCCAGGGCTGGAGGTGACTGCCTGCGTG
CTGCTCTCCACCAAGGCTGTGTACTTTGTGCTCCACGACGGCCTCCGCCGCTACTTCTCA
GAGCCACTGCAGGATTTCTGGCATCAGAAAAACACCGACTACAACAACAGCCCTTTCCAC
ATCTCCCAGTGCTTCGTGCTAAAGCTTAGTGACCTGCAGTCAGTCAATGTGGGGCTTTTC
GACCAGCATTTCCGGCTGACGGGTTCCACCCCGATGCAGGTGGTCACGTGCTTGACGCGG
GACAGCTACCTGACGCACTGCTTCCTCCAGCACCTCATGGTCGTGCTGTCCTCTCTGGAA
CGCACGCCCTCGCCGGAGCCTGTTGACAAGGACTTCTACTCCGAGTTTGGGAACAAGACC
ACAGGGAAGATGGAGAACTACGAGCTGATCCACTCTAGTCGCGTCAAGTTTACCTACCCC
AGTGAGGAGGAGATTGGGGACCTGACGTTCACTGTGGCCCAAAAGATGGCTGAGCCAGAG
AAGGCCCCAGCCCTCAGCATCCTGCTGTACGTGCAGGCCTTCCAGGTGGGCATGCCACCC
CCTGGGTGCTGCAGGGGCCCCCTGCGCCCCAAGACACTCCTGCTCACCAGCTCCGAGATC
TTCCTCCTGGATGAGGACTGTGTCCACTACCCACTGCCCGAGTTTGCCAAAGAGCCGCCG
CAGAGAGACAGGTACCGGCTGGACGATGGCCGCCGCGTCCGGGACCTGGACCGAGTGCTC
ATGGGCTACCAGACCTACCCGCAGGCCCTCACCCTCGTCTTCGATGACGTGCAAGGTCAT
GACCTCATGGGCAGTGTCACCCTGGACCACTTTGGGGAGGTGCCAGGTGGCCCGGCTAGA
GCCAGCCAGGGCCGTGAAGTCCAGTGGCAGGTGTTTGTCCCCAGTGCTGAGAGCAGAGAG
AAGCTCATCTCGCTGTTGGCTCGCCAGTGGGAGGCCCTGTGTGGCCGTGAGCTGCCTGTC
GAGCTCACCGGC

ORF Protein

Cloned ORF protein sequence for pFN21ASDA0975 Download

>KIAA0975  1504 aa
MATARTFGPEREAEPAKEARVVGSELVDTYTVYIIQVTDGSHEWTVKHRYSDFHDLHEKL
VAERKIDKNLLPPKKIIGKNSRSLVEKREKDLEVYLQKLLAAFPGVTPRVLAHFLHFHFY
EINGITAALAEELFEKGEQLLGAGEVFAIGPLQLYAVTEQLQQGKPTCASGDAKTDLGHI
LDFTCRLKYLKVSGTEGPFGTSNIQEQLLPFDLSIFKSLHQVEISHCDAKHIRGLVASKP
TLATLSVRFSATSMKEVLVPEASEFDEWEPEGTTLEGPVTAVIPTWQALTTLDLSHNSIS
EIDESVKLIPKIEFLDLSHNGLLVVDNLQHLYNLVHLDLSYNKLSSLEGLHTKLGNIKTL
NLAGNLLESLSGLHKLYSLVNLDLRDNRIEQMEEVRSIGSLPCLEHVSLLNNPLSIIPDY
RTKVLAQFGERASEVCLDDTVTTEKELDTVEVLKAIQKAKEVKSKLSNPEKKGGEDSRLS
AAPCIRPSSSPPTVAPASASLPQPILSNQGIMFVQEEALASSLSSTDSLTPEHQPIAQGC
SDSLESIPAGQAASDDLRDVPGAVGGASPEHAEPEVQVVPGSGQIIFLPFTCIGYTATNQ
DFIQRLSTLIRQAIERQLPAWIEAANQREEGQGEQGEEEDEEEEEEEDVAENRYFEMGPP
DVEEEEGGGQGEEEEEEEEDEEAEEERLALEWALGADEDFLLEHIRILKVLWCFLIHVQG
SIRQFAACLVLTDFGIAVFEIPHQESRGSSQHILSSLRFVFCFPHGDLTEFGFLMPELCL
VLKVRHSENTLFIISDAANLHEFHADLRSCFAPQHMAMLCSPILYGSHTSLQEFLRQLLT
FYKVAGGCQERSQGCFPVYLVYSDKRMVQTAAGDYSGNIEWASCTLCSAVRRSCCAPSEA
VKSAAIPYWLLLTPQHLNVIKADFNPMPNRGTHNCRNRNSFKLSRVPLSTVLLDPTRSCT
QPRGAFADGHVLELLVGYRFVTAIFVLPHEKFHFLRVYNQLRASLQDLKTVVIAKTPGTG
GSAQGSFADGQPAERRASNDQRPQEVPAEALAPAPVEVPAPAPAAASASGPAKTPAPAEA
STSALVPEETPVEAPAPPPAEAPAQYPSEHLIQATSEENQIPSHLPACPSLRHVASLRGS
AIIELFHSSIAEVENEELRHLMWSSVVFYQTPGLEVTACVLLSTKAVYFVLHDGLRRYFS
EPLQDFWHQKNTDYNNSPFHISQCFVLKLSDLQSVNVGLFDQHFRLTGSTPMQVVTCLTR
DSYLTHCFLQHLMVVLSSLERTPSPEPVDKDFYSEFGNKTTGKMENYELIHSSRVKFTYP
SEEEIGDLTFTVAQKMAEPEKAPALSILLYVQAFQVGMPPPGCCRGPLRPKTLLLTSSEI
FLLDEDCVHYPLPEFAKEPPQRDRYRLDDGRRVRDLDRVLMGYQTYPQALTLVFDDVQGH
DLMGSVTLDHFGEVPGGPARASQGREVQWQVFVPSAESREKLISLLARQWEALCGRELPV
ELTG

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA0975 Download

>pFN21ASDA0975  9241 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGGCGACCGCGCGCAC
CTTCGGGCCCGAGCGGGAAGCCGAGCCGGCCAAGGAAGCGCGCGTCGTGGGCTCGGAGCT
TGTGGACACTTATACGGTTTACATCATCCAGGTCACTGATGGCAGCCATGAGTGGACAGT
AAAGCACCGCTACAGCGACTTCCATGACCTGCATGAAAAGCTCGTTGCAGAGAGAAAGAT
TGATAAAAACCTGCTTCCGCCCAAAAAGATAATTGGGAAAAACTCAAGAAGCTTGGTGGA
GAAGAGGGAGAAGGATCTGGAGGTCTACCTCCAGAAGCTCCTGGCTGCCTTCCCTGGCGT
GACCCCCAGAGTACTGGCCCACTTCTTGCATTTTCACTTCTATGAGATAAATGGCATCAC
CGCGGCACTGGCTGAAGAGCTCTTTGAGAAAGGAGAACAGCTCCTGGGGGCCGGCGAGGT
CTTTGCCATTGGACCCCTGCAGCTGTATGCCGTCACGGAGCAGCTGCAGCAGGGAAAGCC
CACGTGCGCCAGTGGGGATGCCAAGACCGACCTCGGGCACATCCTGGACTTCACCTGTCG
CCTTAAGTACCTTAAGGTTTCTGGCACAGAAGGACCTTTTGGGACCAGCAACATTCAGGA
GCAGCTCCTGCCGTTCGACCTATCAATATTCAAGTCCCTGCATCAGGTGGAGATAAGTCA
CTGTGATGCTAAGCACATCAGAGGGCTGGTCGCATCGAAGCCCACCTTAGCCACGCTGAG
TGTCCGCTTCTCAGCAACCTCGATGAAGGAAGTCCTTGTTCCTGAAGCCTCAGAATTTGA
TGAGTGGGAGCCTGAAGGCACAACCCTAGAAGGCCCTGTGACTGCCGTCATCCCCACTTG
GCAGGCATTGACCACGCTTGACCTGAGCCACAACAGCATCTCCGAGATCGACGAGTCTGT
GAAACTGATCCCAAAGATTGAGTTCCTGGACCTGAGTCACAATGGATTGCTGGTTGTGGA
CAATCTGCAGCACCTGTATAACCTTGTGCATCTGGACCTGTCCTACAACAAGCTCTCCTC
CTTGGAAGGGCTTCACACCAAGCTGGGGAACATCAAGACCTTAAACCTGGCAGGCAACCT
CCTAGAGAGTCTGAGTGGCCTGCACAAGCTCTACTCACTGGTCAACCTGGATCTCCGGGA
CAACAGGATCGAACAGATGGAGGAGGTCCGGAGCATAGGCAGCCTCCCGTGTCTGGAGCA
CGTGTCTCTGCTGAACAACCCTCTGAGCATCATCCCCGACTACCGGACCAAGGTGCTGGC
TCAGTTCGGAGAGAGGGCCTCAGAGGTCTGTCTGGATGACACAGTGACCACAGAGAAGGA
GCTGGACACTGTGGAAGTGCTGAAAGCAATTCAGAAAGCCAAGGAGGTCAAGTCCAAACT
GAGCAACCCAGAGAAGAAGGGTGGTGAAGACTCCCGGCTCTCAGCTGCCCCCTGCATCAG
ACCCAGCAGCTCCCCTCCCACTGTGGCTCCCGCATCTGCCTCCCTGCCCCAGCCCATCCT
CTCTAACCAAGGAATCATGTTCGTTCAGGAGGAGGCCCTGGCCAGCAGCCTCTCGTCCAC
TGACAGTCTGACTCCCGAGCACCAGCCCATTGCCCAGGGATGTTCTGATTCCTTGGAGTC
CATCCCTGCGGGACAGGCAGCTTCCGATGATTTAAGGGACGTGCCAGGAGCTGTTGGTGG
TGCAAGCCCAGAACATGCCGAGCCGGAGGTCCAGGTGGTGCCGGGGTCTGGCCAGATCAT
CTTCCTGCCCTTCACCTGCATTGGCTACACGGCCACCAATCAGGACTTCATCCAGCGCCT
GAGCACACTGATCCGGCAGGCCATCGAGCGGCAGCTGCCTGCCTGGATCGAGGCTGCCAA
CCAGCGGGAGGAGGGCCAGGGTGAACAGGGCGAGGAGGAGGATGAGGAGGAGGAAGAAGA
GGAGGACGTGGCTGAGAACCGCTACTTTGAAATGGGGCCCCCAGACGTGGAGGAGGAGGA
GGGAGGAGGCCAGGGGGAGGAAGAGGAGGAGGAAGAGGAGGATGAAGAGGCCGAGGAGGA
GCGCCTGGCTCTGGAATGGGCCCTGGGCGCGGACGAGGACTTCCTGCTGGAGCACATCCG
CATCCTCAAGGTGCTGTGGTGCTTCCTGATCCATGTGCAGGGCAGTATCCGCCAGTTCGC
CGCCTGCCTTGTGCTCACCGACTTCGGCATCGCAGTCTTCGAGATCCCGCACCAGGAGTC
TCGGGGCAGCAGCCAGCACATCCTCTCCTCCCTGCGCTTTGTCTTTTGCTTCCCGCATGG
CGACCTCACCGAGTTTGGCTTCCTCATGCCGGAGCTGTGTCTGGTGCTCAAGGTACGGCA
CAGTGAGAACACGCTCTTCATTATCTCGGACGCCGCCAACCTGCACGAGTTCCACGCGGA
CCTGCGCTCATGCTTTGCACCCCAGCACATGGCCATGCTGTGTAGCCCCATCCTCTACGG
CAGCCACACCAGCCTGCAGGAGTTCCTGCGCCAGCTGCTCACCTTCTACAAGGTGGCTGG
CGGCTGCCAGGAGCGCAGCCAGGGCTGCTTCCCCGTCTACCTGGTCTACAGTGACAAGCG
CATGGTGCAGACGGCCGCCGGGGACTACTCAGGCAACATCGAGTGGGCCAGCTGCACACT
CTGTTCAGCCGTGCGGCGCTCCTGCTGCGCGCCCTCTGAGGCCGTCAAGTCCGCCGCCAT
CCCCTACTGGCTGTTGCTCACGCCCCAGCACCTCAACGTCATCAAGGCCGACTTCAACCC
CATGCCCAACCGTGGCACCCACAACTGTCGCAACCGCAACAGCTTCAAGCTCAGCCGTGT
GCCGCTCTCCACCGTGCTGCTGGACCCCACACGCAGCTGTACCCAGCCTCGGGGCGCCTT
TGCTGATGGCCACGTGCTAGAGCTGCTCGTGGGGTACCGCTTTGTCACTGCCATCTTCGT
GCTGCCCCACGAGAAGTTCCACTTCCTGCGCGTCTACAACCAGCTGCGGGCCTCGCTGCA
GGACCTGAAGACTGTGGTCATCGCCAAGACCCCCGGGACGGGAGGCAGCGCCCAGGGCTC
CTTTGCGGATGGCCAGCCTGCCGAGCGCAGGGCCAGCAATGACCAGCGTCCCCAGGAGGT
CCCAGCAGAGGCTCTGGCCCCGGCCCCAGTGGAAGTCCCAGCTCCAGCCCCTGCAGCAGC
CTCAGCCTCAGGCCCAGCGAAGACTCCGGCCCCAGCAGAGGCCTCAACTTCAGCTTTGGT
CCCAGAGGAGACGCCAGTGGAAGCTCCAGCCCCACCCCCAGCCGAGGCCCCTGCCCAGTA
CCCGAGTGAGCACCTCATCCAGGCCACCTCGGAGGAGAATCAGATCCCCTCGCACTTGCC
TGCCTGCCCGTCGCTCCGGCACGTCGCCAGCCTGCGGGGCAGCGCCATCATCGAGCTCTT
CCACAGCAGCATTGCTGAGGTTGAAAACGAGGAGCTGAGGCACCTCATGTGGTCCTCGGT
GGTGTTCTACCAGACCCCAGGGCTGGAGGTGACTGCCTGCGTGCTGCTCTCCACCAAGGC
TGTGTACTTTGTGCTCCACGACGGCCTCCGCCGCTACTTCTCAGAGCCACTGCAGGATTT
CTGGCATCAGAAAAACACCGACTACAACAACAGCCCTTTCCACATCTCCCAGTGCTTCGT
GCTAAAGCTTAGTGACCTGCAGTCAGTCAATGTGGGGCTTTTCGACCAGCATTTCCGGCT
GACGGGTTCCACCCCGATGCAGGTGGTCACGTGCTTGACGCGGGACAGCTACCTGACGCA
CTGCTTCCTCCAGCACCTCATGGTCGTGCTGTCCTCTCTGGAACGCACGCCCTCGCCGGA
GCCTGTTGACAAGGACTTCTACTCCGAGTTTGGGAACAAGACCACAGGGAAGATGGAGAA
CTACGAGCTGATCCACTCTAGTCGCGTCAAGTTTACCTACCCCAGTGAGGAGGAGATTGG
GGACCTGACGTTCACTGTGGCCCAAAAGATGGCTGAGCCAGAGAAGGCCCCAGCCCTCAG
CATCCTGCTGTACGTGCAGGCCTTCCAGGTGGGCATGCCACCCCCTGGGTGCTGCAGGGG
CCCCCTGCGCCCCAAGACACTCCTGCTCACCAGCTCCGAGATCTTCCTCCTGGATGAGGA
CTGTGTCCACTACCCACTGCCCGAGTTTGCCAAAGAGCCGCCGCAGAGAGACAGGTACCG
GCTGGACGATGGCCGCCGCGTCCGGGACCTGGACCGAGTGCTCATGGGCTACCAGACCTA
CCCGCAGGCCCTCACCCTCGTCTTCGATGACGTGCAAGGTCATGACCTCATGGGCAGTGT
CACCCTGGACCACTTTGGGGAGGTGCCAGGTGGCCCGGCTAGAGCCAGCCAGGGCCGTGA
AGTCCAGTGGCAGGTGTTTGTCCCCAGTGCTGAGAGCAGAGAGAAGCTCATCTCGCTGTT
GGCTCGCCAGTGGGAGGCCCTGTGTGGCCGTGAGCTGCCTGTCGAGCTCACCGGCTACGT
AGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGC
AAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTG
AGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATT
GATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATT
TGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAAC
AATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAG
TAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTT
TCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGA
CTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGC
GGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAA
TAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTC
CGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAA
ACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAA
CTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATG
ATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAA
GAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTC
ACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACC
ATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTA
ACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAG
CTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACA
ACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATA
GACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGC
TGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCA
CTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCA
ACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGG
TAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAA
TACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGC
AAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCC
CTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTAT
AAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGC
CGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCT
CACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACG
AACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACC
CGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGA
GGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAA
GGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTA
GCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGC
AGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTG
ACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGA
TCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCG
CTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGC
CTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGC
AGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGC
GTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTA
GCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATC
T