Restriction map for
pFN21ASDA0975
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# NO CUT ENZYMES ( 70 enzymes) #
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AatII AclI AgeI Alw44I Aor51HI
ApaLI AscI AseI AsnI AviII
AvrII BamHI BclI BlnI BsaBI
BsaI BsiWI BsmI Bsp1407I BspDI
BspLU11I BsrGI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DraIII DrdI Eco47III EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HpaI MamI MfeI MluI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RsrII
SalI SfiI SfuI SgrAI SnaBI
SpeI SspBI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccIII 1184 TCCGGA T^CCGGA
AcsI 790 GAATTT [GA]^AATT[CT]
AflIII 1217 ACGTGT A^C[GA][CT]GT
AhdI 3691 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3203 CCTCAGG CC^T[ACGT]AGG
ApoI 790 GAATTT [GA]^AATT[CT]
Asp700I 1042 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3691 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4156 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 2890 GGCGCC GGCGC^C
BciVI 2162 GTATCC GTATCC(6/5)
BglII 4136 AGATCT A^GATCT
BseAI 1184 TCCGGA T^CCGGA
BsmBI 3265 GAGACG CGTCTC(1/5)
BspEI 1184 TCCGGA T^CCGGA
BspHI 4317 TCATGA T^CATGA
BstAPI 618 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 3203 CCTCAGG CC^T[ACGT]AGG
EagI 2609 CGGCCG C^GGCCG
Eam1105I 3691 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2609 CGGCCG C^GGCCG
Eco52I 2609 CGGCCG C^GGCCG
Eco81I 3203 CCTCAGG CC^T[ACGT]AGG
KasI 2890 GGCGCC G^GCGCC
KspI 377 CCGCGG CCGC^GG
MroI 1184 TCCGGA T^CCGGA
NarI 2890 GGCGCC GG^CGCC
PflFI 4156 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 4317 TCATGA T^CATGA
SacII 377 CCGCGG CCGC^GG
SbfI 2490 CCTGCAGG CCTGCA^GG
ScaI 327 AGTACT AGT^ACT
SexAI 2576 ACCTGGT A^CC[AT]GGT
SfoI 2890 GGCGCC GGC^GCC
SmaI 3049 CCCGGG CCC^GGG
SphI 4070 GCATGC GCATG^C
Sse8387I 2490 CCTGCAGG CCTGCA^GG
SspI 642 AATATT AAT^ATT
Tth111I 4156 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 3049 CCCGGG C^CCGGG
XmaI 3049 CCCGGG C^CCGGG
XmnI 1042 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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Acc65I 2930 GGTACC G^GTACC
4211 GGTACC
AflII 559 CTTAAG C^TTAAG
568 CTTAAG
Asp718I 2930 GGTACC G^GTACC
4211 GGTACC
BcgI 432 CGAGGTCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3300 CGAGGCCCCTGC
BfrI 559 CTTAAG C^TTAAG
568 CTTAAG
BglI 37 GCCGAGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3407 GCCTGCGGGGC
BsaWI 1184 TCCGGA [AT]^CCGG[AT]
1259 ACCGGA
BsiEI 5 CGACCG CG[GA][CT]^CG
2609 CGGCCG
BstXI 323 CCAGAGTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1265 CCAAGGTGCTGG
DsaI 377 CCGCGG C^C[GA][CT]GG
2787 CCGTGG
HincII 1138 GTCAAC GT[CT]^[GA]AC
3862 GTTGAC
HindII 1138 GTCAAC GT[CT]^[GA]AC
3862 GTTGAC
HindIII 246 AAGCTT A^AGCTT
3682 AAGCTT
KpnI 2930 GGTACC GGTAC^C
4211 GGTACC
NaeI 42 GCCGGC GCC^GGC
427 GCCGGC
NgoMIV 42 GCCGGC G^CCGGC
427 GCCGGC
NspI 1710 ACATGC [GA]CATG^[CT]
4070 GCATGC
StuI 3236 AGGCCT AGG^CCT
4055 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbrPI BbsI BbvCI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssHII BssKI
BssSI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PmaCI PmlI PpuMI PshAI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII