(Circular) (Six-base) MAPSORT of: pFN21ASDA0975.seq Check: 1166 from: 1 to: 9241 pFN21ASDA0975 9241 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3803 5770 6421 3803 Size: 1967 651 6623 Fragments arranged by size: 6623 1967 651 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7051 278 Size: 53 83 186 936 5515 2468 Fragments arranged by size: 5515 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2304 4596 4605 5013 6584 1937 Size: 367 2292 9 408 1571 4594 Fragments arranged by size: 4594 2292 1571 408 367 9 Acc65I G'GTAC_C Cuts at: 4953 6234 6562 4953 Size: 1281 328 7632 Fragments arranged by size: 7632 1281 328 AclI AA'CG_TT Cuts at: 1563 7368 7741 1563 Size: 5805 373 3063 Fragments arranged by size: 5805 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2433 2823 2853 5066 5253 7304 8688 8956 Size: 801 390 30 2213 187 2051 1384 268 Cuts at: 8956 1632 Size: 1917 Fragments arranged by size: 2213 2051 1917 1384 801 390 268 187 30 AflII C'TTAA_G Cuts at: 820 1017 2582 2591 820 Size: 197 1565 9 7470 Fragments arranged by size: 7470 1565 197 9 AflIII A'CryG_T Cuts at: 1203 3240 8140 9117 1203 Size: 2037 4900 977 1327 Fragments arranged by size: 4900 2037 1327 977 AgeI A'CCGG_T Cuts at: 8072 8072 Size: 9241 AhdI GACnn_n'nnGTC Cuts at: 5719 7970 5719 Size: 2251 6990 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9241 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2502 3894 5415 1184 Size: 1318 1392 1521 5010 Fragments arranged by size: 5010 1521 1392 1318 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5993 6025 5993 Size: 32 9209 AlwNI CAG_nnn'CTG Cuts at: 2443 2647 3013 3710 3838 5044 5210 6984 8556 Size: 204 366 697 128 1206 166 1774 1572 Cuts at: 8556 2443 Size: 3128 Fragments arranged by size: 3128 1774 1572 1206 697 366 204 166 128 ApaI G_GGCC'C Cuts at: 2049 3999 4102 6122 2049 Size: 1950 103 2020 5168 Fragments arranged by size: 5168 2020 1950 103 ApaLI G'TGCA_C Cuts at: 7298 8454 7298 Size: 1156 8085 ApoI r'AATT_y Cuts at: 1493 2813 6550 6776 6928 9037 1493 Size: 1320 3737 226 152 2109 1697 Fragments arranged by size: 3737 2109 1697 1320 226 152 AseI AT'TA_AT Cuts at: 160 1032 7795 160 Size: 872 6763 1606 Fragments arranged by size: 6763 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9241 AvaI C'yCGr_G Cuts at: 1870 1943 2048 2552 3614 4260 4908 5072 5341 Size: 73 105 504 1062 646 648 164 269 Cuts at: 5341 6200 6566 1870 Size: 859 366 4545 Fragments arranged by size: 4545 1062 859 648 646 504 366 269 164 105 73 BamHI G'GATC_C Cuts at: 6571 6938 6571 Size: 367 8874 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3757 4814 4913 4953 5499 Size: 325 217 627 1970 1057 99 40 546 Cuts at: 5499 6234 6382 6562 8017 618 Size: 735 148 180 1455 1842 Fragments arranged by size: 1970 1842 1455 1057 735 627 546 325 217 180 148 99 40 BanII G_rGCy'C Cuts at: 1391 2049 2093 2421 3999 4102 5099 5457 6122 Size: 658 44 328 1578 103 997 358 665 Cuts at: 6122 6528 6560 1391 Size: 406 32 4072 Fragments arranged by size: 4072 1578 997 665 658 406 358 328 103 44 32 BbeI G_GCGC'C Cuts at: 4917 4917 Size: 9241 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3453 4228 5054 5246 6313 928 Size: 971 1554 775 826 192 1067 3856 Fragments arranged by size: 3856 1554 1067 971 826 775 192 BbvCI CC'TCA_GC Cuts at: 5221 5475 5495 6056 5221 Size: 254 20 561 8406 Fragments arranged by size: 8406 561 254 20 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2444 2478 5312 5346 7432 7466 1903 Size: 34 507 34 2834 34 2086 34 3678 Fragments arranged by size: 3678 2834 2086 507 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4196 7133 8343 4196 Size: 2937 1210 5094 Fragments arranged by size: 5094 2937 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 9241 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2066 5436 7852 Size: 107 122 71 631 605 394 3370 2416 Cuts at: 7852 136 Size: 1525 Fragments arranged by size: 3370 2416 1525 631 605 394 122 107 71 BglII A'GATC_T Cuts at: 6159 9236 6159 Size: 3077 6164 BlpI GC'TnA_GC Cuts at: 2709 4850 6033 6658 2709 Size: 2141 1183 625 5292 Fragments arranged by size: 5292 2141 1183 625 Bme1580I G_kGCm'C Cuts at: 1790 2049 2559 3999 4102 6122 7302 8458 1790 Size: 259 510 1440 103 2020 1180 1156 2573 Fragments arranged by size: 2573 2020 1440 1180 1156 510 259 103 BmgBI CAC'GTC Cuts at: 1318 3700 3967 4006 5422 6331 1318 Size: 2382 267 39 1416 909 4228 Fragments arranged by size: 4228 2382 1416 909 267 39 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5180 5329 5683 Size: 416 53 83 399 24 3763 149 354 Cuts at: 5683 5976 6438 6972 7930 442 Size: 293 462 534 958 1753 Fragments arranged by size: 3763 1753 958 534 462 416 399 354 293 149 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9241 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2456 3334 6482 2456 Size: 878 3148 5215 Fragments arranged by size: 5215 3148 878 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2294 2318 3254 4148 5187 5289 5564 5813 Size: 1055 24 936 894 1039 102 275 249 Cuts at: 5813 7901 1239 Size: 2088 2579 Fragments arranged by size: 2579 2088 1055 1039 936 894 275 249 102 24 Bpu10I CC'TnA_GC Cuts at: 2746 2903 3839 5221 5475 5495 6056 2746 Size: 157 936 1382 254 20 561 5931 Fragments arranged by size: 5931 1382 936 561 254 157 20 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2248 4130 4352 7366 8231 8529 2248 Size: 1882 222 3014 865 298 2960 Fragments arranged by size: 3014 2960 1882 865 298 222 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 7904 882 Size: 617 6405 2219 Fragments arranged by size: 6405 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2523 3241 4933 5790 6073 6538 7032 Size: 711 1319 718 1692 857 283 465 494 Cuts at: 7032 493 Size: 2702 Fragments arranged by size: 2702 1692 1319 857 718 711 494 465 283 BsaBI GATnn'nnATC Cuts at: 9235 9235 Size: 9241 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4410 4914 5291 7048 Size: 53 83 186 936 2877 504 377 1757 Cuts at: 7048 7430 8065 275 Size: 382 635 1451 Fragments arranged by size: 2877 1757 1451 936 635 504 382 377 186 83 53 BsaWI w'CCGG_w Cuts at: 3207 3282 7674 8072 8346 8493 8904 3207 Size: 75 4392 398 274 147 411 3544 Fragments arranged by size: 4392 3544 411 398 274 147 75 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2903 2933 3038 3068 3227 Size: 30 165 30 1500 30 105 30 159 Cuts at: 3227 3257 3395 3425 3490 3520 4268 4298 5885 Size: 30 138 30 65 30 748 30 1587 Cuts at: 5885 5915 6485 6515 1178 Size: 30 570 30 3904 Fragments arranged by size: 3904 1587 1500 748 570 165 159 138 105 65 30 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3046 3215 3584 3923 3947 3950 3959 3962 Size: 1860 169 369 339 24 3 9 3 Cuts at: 3962 3974 4025 4028 4031 4038 4058 4061 4070 Size: 12 51 3 3 7 20 3 9 Cuts at: 4070 4088 4091 4276 5300 5387 5504 6002 6005 Size: 18 3 185 1024 87 117 498 3 Cuts at: 6005 1186 Size: 4422 Fragments arranged by size: 4422 1860 1024 498 369 339 185 169 117 87 51 24 20 18 12 9 9 7 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 3082 3482 3528 4461 4764 4902 5161 5197 5233 Size: 400 46 933 303 138 259 36 36 Cuts at: 5233 5251 5317 6049 8444 8940 3082 Size: 18 66 732 2395 496 3383 Fragments arranged by size: 3383 2395 933 732 496 400 303 259 138 66 46 36 36 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3125 4196 4406 4646 4657 6095 1163 Size: 573 1389 1071 210 240 11 1438 4309 Fragments arranged by size: 4309 1438 1389 1071 573 240 210 11 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2031 4635 6691 7452 8480 1540 Size: 275 186 30 2604 2056 761 1028 2301 Fragments arranged by size: 2604 2301 2056 1028 761 275 186 30 BsiHKAI G_wGCw'C Cuts at: 2421 3240 3621 3845 4134 4215 4368 5352 5457 Size: 819 381 224 289 81 153 984 105 Cuts at: 5457 5595 6282 6528 7302 7387 8458 2421 Size: 138 687 246 774 85 1071 3204 Fragments arranged by size: 3204 1071 984 819 774 687 381 289 246 224 153 138 105 85 81 BsmI GAATG_Cn' Cuts at: 6752 6845 6752 Size: 93 9148 BsmBI CGTCTCn'nnnn_ Cuts at: 5282 5282 Size: 9241 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2049 2093 2421 2559 3240 3621 3845 Size: 399 259 44 328 138 681 381 224 Cuts at: 3845 3999 4102 4134 4215 4368 5099 5352 5457 Size: 154 103 32 81 153 731 253 105 Cuts at: 5457 5595 6122 6282 6528 6560 7302 7387 8458 Size: 138 527 160 246 32 742 85 1071 Cuts at: 8458 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 731 681 527 399 381 328 259 253 246 224 160 154 153 138 138 105 103 85 81 44 32 32 BspEI T'CCGG_A Cuts at: 3207 8904 3207 Size: 5697 3544 BspHI T'CATG_A Cuts at: 6340 7130 8860 6340 Size: 790 1730 6721 Fragments arranged by size: 6721 1730 790 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2196 2238 3803 4428 4449 5724 5770 6421 Size: 1352 42 1565 625 21 1275 46 651 Cuts at: 6421 6596 844 Size: 175 3489 Fragments arranged by size: 3489 1565 1352 1275 651 625 175 46 42 21 BsrBI CCG'CTC Cuts at: 1394 1823 2053 3868 4864 7128 1394 Size: 429 230 1815 996 2264 3507 Fragments arranged by size: 3507 2264 1815 996 429 230 BsrDI GCAATG_nn' Cuts at: 65 1248 3627 5143 5469 7736 7910 65 Size: 1183 2379 1516 326 2267 174 1396 Fragments arranged by size: 2379 2267 1516 1396 1183 326 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2065 2450 6237 6530 7885 8072 1700 Size: 110 255 385 3787 293 1355 187 2869 Fragments arranged by size: 3787 2869 1355 385 293 255 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9241 BssHII G'CGCG_C Cuts at: 1925 2033 2078 4707 1925 Size: 108 45 2629 6459 Fragments arranged by size: 6459 2629 108 45 BssSI C'ACGA_G Cuts at: 4424 4946 4988 7301 8313 4424 Size: 522 42 2313 1012 5352 Fragments arranged by size: 5352 2313 1012 522 42 BstAPI GCAn_nnn'nTGC Cuts at: 794 2647 794 Size: 1853 7388 BstBI TT'CG_AA Cuts at: 2007 8045 2007 Size: 6038 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9241 BstXI CCAn_nnnn'nTGG Cuts at: 2353 3295 2353 Size: 942 8299 BstYI r'GATC_y Cuts at: 1972 2293 3169 4242 5382 6159 6571 6938 7323 Size: 321 876 1073 1140 777 412 367 385 Cuts at: 7323 7340 8792 8878 8890 9236 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1140 1073 876 777 412 385 367 346 321 86 17 12 Bsu36I CC'TnA_GG Cuts at: 5227 5227 Size: 9241 BtgI C'CryG_G Cuts at: 513 1065 2022 2400 4810 513 Size: 552 957 378 2410 4944 Fragments arranged by size: 4944 2410 957 552 378 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4213 5044 487 Size: 683 56 2987 831 4684 Fragments arranged by size: 4684 2987 831 683 56 BtsI GCAGTG_nn' Cuts at: 4965 5627 5818 6194 6361 6757 7551 7571 4965 Size: 662 191 376 167 396 794 20 6635 Fragments arranged by size: 6635 794 662 396 376 191 167 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9241 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9206 DraI TTT'AAA Cuts at: 6545 6545 Size: 9241 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8248 809 Size: 507 6932 1802 Fragments arranged by size: 6932 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2067 3579 3769 Size: 54 1425 50 275 18 237 1512 190 Cuts at: 3769 3810 4470 4632 4927 5110 6250 6505 6688 Size: 41 660 162 295 183 1140 255 183 Cuts at: 6688 7577 9206 8 Size: 889 1629 43 Fragments arranged by size: 1629 1512 1425 1140 889 660 295 275 255 237 190 183 183 162 54 50 43 41 18 EagI C'GGCC_G Cuts at: 1537 1812 4632 6688 1537 Size: 275 2820 2056 4090 Fragments arranged by size: 4090 2820 2056 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2276 2409 3951 4035 4047 4059 4400 5462 Size: 923 133 1542 84 12 12 341 1062 Cuts at: 5462 7171 1353 Size: 1709 3423 Fragments arranged by size: 3423 1709 1542 1062 923 341 133 84 12 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2226 4178 4720 5597 7828 8202 Size: 1064 138 637 1952 542 877 2231 374 Cuts at: 8202 8348 387 Size: 146 1280 Fragments arranged by size: 2231 1952 1280 1064 877 637 542 374 146 138 EcoICRI GAG'CTC Cuts at: 2419 5455 6526 2419 Size: 3036 1071 5134 Fragments arranged by size: 5134 3036 1071 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2294 2318 2433 2823 2853 3254 4148 Size: 393 662 24 115 390 30 401 894 Cuts at: 4148 5066 5187 5253 5289 5564 5813 7304 7901 Size: 918 121 66 36 275 249 1491 597 Cuts at: 7901 8688 8956 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1491 918 894 787 662 597 401 393 390 275 268 249 121 115 66 36 30 24 EcoNI CCTnn'n_nnAGG Cuts at: 2681 5357 6173 2681 Size: 2676 816 5749 Fragments arranged by size: 5749 2676 816 EcoO109I rG'GnC_Cy Cuts at: 2612 2852 3315 3574 3995 3996 4099 5041 5158 Size: 240 463 259 421 1 103 942 117 Cuts at: 5158 5326 6001 6046 6118 6119 6265 6307 6496 Size: 168 675 45 72 1 146 42 189 Cuts at: 6496 2612 Size: 5357 Fragments arranged by size: 5357 942 675 463 421 259 240 189 168 146 117 103 72 45 42 1 1 EcoRI G'AATT_C Cuts at: 6550 6928 6550 Size: 378 8863 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2761 4807 5017 2761 Size: 2046 210 6985 Fragments arranged by size: 6985 2046 210 FspI TGC'GCA Cuts at: 7747 7747 Size: 9241 HaeII r_GCGC'y Cuts at: 1678 3838 4084 4699 4917 5091 5445 8388 1678 Size: 2160 246 615 218 174 354 2943 2531 Fragments arranged by size: 2943 2531 2160 615 354 246 218 174 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2712 2744 3227 3259 3395 Size: 32 135 32 1140 32 483 32 136 Cuts at: 3395 3427 4430 4462 5705 5737 5885 5917 7882 Size: 32 1003 32 1243 32 148 32 1965 Cuts at: 7882 7914 7956 7988 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1965 1243 1140 1003 483 148 136 135 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3163 5887 6585 6831 669 Size: 988 281 1225 2724 698 246 3079 Fragments arranged by size: 3079 2724 1225 988 698 281 246 HindIII A'AGCT_T Cuts at: 748 2269 5705 748 Size: 1521 3436 4284 Fragments arranged by size: 4284 3436 1521 HpaI GTT'AAC Cuts at: 6831 6831 Size: 9241 KasI G'GCGC_C Cuts at: 4913 4913 Size: 9241 KpnI G_GTAC'C Cuts at: 4957 6238 6566 4957 Size: 1281 328 7632 Fragments arranged by size: 7632 1281 328 MfeI C'AATT_G Cuts at: 6840 6840 Size: 9241 MluI A'CGCG_T Cuts at: 9117 9117 Size: 9241 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 8355 8539 1305 Size: 7050 184 2007 Fragments arranged by size: 7050 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3581 3771 4472 4929 5112 Size: 54 1425 343 1749 190 701 457 183 Cuts at: 5112 10 Size: 4139 Fragments arranged by size: 4139 1749 1425 701 457 343 190 183 54 MslI CAynn'nnrTG Cuts at: 518 1166 2379 2703 4472 4976 5774 5843 7199 Size: 648 1213 324 1769 504 798 69 1356 Cuts at: 7199 7558 7717 518 Size: 359 159 2042 Fragments arranged by size: 2042 1769 1356 1213 798 648 504 359 324 159 69 MspA1I CmG'CkG Cuts at: 1606 2402 2482 2503 3464 3874 3904 4534 4671 Size: 796 80 21 961 410 30 630 137 Cuts at: 4671 4896 5023 6657 7334 8482 8727 1606 Size: 225 127 1634 677 1148 245 2120 Fragments arranged by size: 2120 1634 1148 961 796 677 630 410 245 225 137 127 80 30 21 NaeI GCC'GGC Cuts at: 2067 2452 2067 Size: 385 8856 NarI GG'CG_CC Cuts at: 4914 4914 Size: 9241 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 7732 Fragments arranged by size: 7732 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9241 NgoMIV G'CCGG_C Cuts at: 2065 2450 2065 Size: 385 8856 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9241 NotI GC'GGCC_GC Cuts at: 6688 6688 Size: 9241 NspI r_CATG'y Cuts at: 1132 1229 3737 6097 6599 8144 1132 Size: 97 2508 2360 502 1545 2229 Fragments arranged by size: 2508 2360 2229 1545 502 97 PasI CC'CwG_GG Cuts at: 1348 3634 4102 5092 5537 6104 1348 Size: 2286 468 990 445 567 4485 Fragments arranged by size: 4485 2286 990 567 468 445 PciI A'CATG_T Cuts at: 8140 8140 Size: 9241 PflMI CCAn_nnn'nTGG Cuts at: 1108 4468 6028 6104 1108 Size: 3360 1560 76 4245 Fragments arranged by size: 4245 3360 1560 76 PfoI T'CCnGG_A Cuts at: 2562 2964 3174 6168 6261 2562 Size: 402 210 2994 93 5542 Fragments arranged by size: 5542 2994 402 210 93 PmeI GTTT'AAAC Cuts at: 6545 6545 Size: 9241 PmlI CAC'GTG Cuts at: 2523 3241 4933 5790 2523 Size: 718 1692 857 5974 Fragments arranged by size: 5974 1692 857 718 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7310 7342 8823 8855 7310 Size: 32 1481 32 7696 Fragments arranged by size: 7696 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2612 5041 5158 6001 6265 2612 Size: 2429 117 843 264 5588 Fragments arranged by size: 5588 2429 843 264 117 PshAI GACnn'nnGTC Cuts at: 1613 2864 2973 5054 5145 1613 Size: 1251 109 2081 91 5709 Fragments arranged by size: 5709 2081 1251 109 91 PsiI TTA'TAA Cuts at: 6811 6811 Size: 9241 PspOMI G'GGCC_C Cuts at: 2045 3995 4098 6118 2045 Size: 1950 103 2020 5168 Fragments arranged by size: 5168 2020 1950 103 PstI C_TGCA'G Cuts at: 830 2481 2508 3009 4518 5040 5215 5634 5721 Size: 1651 27 501 1509 522 175 419 87 Cuts at: 5721 6116 6593 830 Size: 395 477 3478 Fragments arranged by size: 3478 1651 1509 522 501 477 419 395 175 87 27 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9241 PvuII CAG'CTG Cuts at: 2482 2503 3464 3874 4534 4671 4896 5023 2482 Size: 21 961 410 660 137 225 127 6700 Fragments arranged by size: 6700 961 660 410 225 137 127 21 SacI G_AGCT'C Cuts at: 2421 5457 6528 2421 Size: 3036 1071 5134 Fragments arranged by size: 5134 3036 1071 SacII CC_GC'GG Cuts at: 2403 2403 Size: 9241 SalI G'TCGA_C Cuts at: 1936 6583 1936 Size: 4647 4594 SapI GCTCTTCn'nnn_ Cuts at: 2409 4400 5462 2409 Size: 1991 1062 6188 Fragments arranged by size: 6188 1991 1062 SbfI CC_TGCA'GG Cuts at: 4518 6593 4518 Size: 2075 7166 ScaI AGT'ACT Cuts at: 2352 7489 9201 2352 Size: 5137 1712 2392 Fragments arranged by size: 5137 2392 1712 SexAI A'CCwGG_T Cuts at: 4599 4599 Size: 9241 SfcI C'TryA_G Cuts at: 826 1045 2170 2477 2504 3005 4514 4606 5036 Size: 219 1125 307 27 501 1509 92 430 Cuts at: 5036 5211 5630 5717 6112 6589 7724 8405 8596 Size: 175 419 87 395 477 1135 681 191 Cuts at: 8596 826 Size: 1471 Fragments arranged by size: 1509 1471 1135 1125 681 501 477 430 419 395 307 219 191 175 92 87 27 SfoI GGC'GCC Cuts at: 4915 4915 Size: 9241 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9241 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9241 SmaI CCC'GGG Cuts at: 1872 5074 6568 1872 Size: 3202 1494 4545 Fragments arranged by size: 4545 3202 1494 SmlI C'TyrA_G Cuts at: 820 1017 1943 2263 2582 2591 4145 4367 7345 Size: 197 926 320 319 9 1554 222 2978 Cuts at: 7345 8246 8508 820 Size: 901 262 1553 Fragments arranged by size: 2978 1554 1553 926 901 320 319 262 222 197 9 SnaBI TAC'GTA Cuts at: 493 6538 7032 493 Size: 6045 494 2702 Fragments arranged by size: 6045 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 9241 SphI G_CATG'C Cuts at: 1132 6097 6599 1132 Size: 4965 502 3774 Fragments arranged by size: 4965 3774 502 SspI AAT'ATT Cuts at: 5 52 2667 7165 5 Size: 47 2615 4498 2081 Fragments arranged by size: 4498 2615 2081 47 StuI AGG'CCT Cuts at: 1517 5261 6080 1517 Size: 3744 819 4678 Fragments arranged by size: 4678 3744 819 StyI C'CwwG_G Cuts at: 513 1065 2022 2070 3060 3288 3399 3547 3651 Size: 552 957 48 990 228 111 148 104 Cuts at: 3651 5574 6680 513 Size: 1923 1106 3074 Fragments arranged by size: 3074 1923 1106 990 957 552 228 148 111 104 48 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2561 4832 6096 6288 7268 7285 7438 Size: 1255 981 2271 1264 192 980 17 153 Cuts at: 7438 7623 8068 325 Size: 185 445 1498 Fragments arranged by size: 2271 1498 1264 1255 981 980 445 192 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2350 5583 7487 Size: 274 80 33 51 1443 373 3233 1904 Cuts at: 7487 9199 96 Size: 1712 138 Fragments arranged by size: 3233 1904 1712 1443 373 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3490 3522 4268 4300 6485 6517 9201 9233 3490 Size: 32 746 32 2185 32 2684 32 3498 Fragments arranged by size: 3498 2684 2185 746 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 6182 1316 Size: 4866 4375 XbaI T'CTAG_A Cuts at: 6577 6577 Size: 9241 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2135 4876 5929 2135 Size: 2741 1053 5447 Fragments arranged by size: 5447 2741 1053 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9241 XmaI C'CCGG_G Cuts at: 1870 5072 6566 1870 Size: 3202 1494 4545 Fragments arranged by size: 4545 3202 1494 XmnI GAAnn'nnTTC Cuts at: 3069 7370 3069 Size: 4301 4940 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7049 276 Size: 53 83 186 936 5515 2468 Fragments arranged by size: 5515 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoRV FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SanDI SfiI SrfI SwaI