NPFFR2 ORF clone pFN21AE9546

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Halotag ORF Clone pFN21AE9546

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Clone info.

Species

human

Clone Name

pFN21AE9546 Sequence (with vector) Restriction Map

Product ID

FHC21952

ORF Sequence

DNA sequence (1566 bp)
Protein sequence (522 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE9546

Symbol

NPFFR2

Alias : GPR74, HLWAR77, NPFF2, NPGPR

Description

neuropeptide FF receptor 2, transcript variant 1

Original Clone ID

cp99546

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1566 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 522 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS3551.1 1.1e-164 100.0 NPFFR2 CCDS47072.1 1.5e-130 100.0 NPFFR2 CCDS3552.1 6.4e-130 100.0 NPFFR2 CCDS53539.1 2.9e-61 56.4 NPFFR1 CCDS3719.1 6.1e-26 31.5 QRFPR The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR005397 106 124 PR01572 Neuropeptide FF receptor IPR005395 133 146 PR01570 Neuropeptide FF receptor IPR000276 149 173 PR00237 GPCR IPR005395 158 170 PR01570 Neuropeptide FF receptor IPR000276 182 203 PR00237 GPCR IPR005397 196 208 PR01572 Neuropeptide FF receptor IPR005395 197 214 PR01570 Neuropeptide FF receptor IPR000276 227 249 PR00237 GPCR IPR005395 246 260 PR01570 Neuropeptide FF receptor IPR000276 261 282 PR00237 GPCR IPR005397 283 303 PR01572 Neuropeptide FF receptor IPR000276 322 345 PR00237 GPCR IPR000276 374 398 PR00237 GPCR IPR005397 398 409 PR01572 Neuropeptide FF receptor IPR005395 413 425 PR01570 Neuropeptide FF receptor IPR000276 417 443 PR00237 GPCR IPR005395 440 450 PR01570 Neuropeptide FF receptor IPR005397 455 470 PR01572 Neuropeptide FF receptor IPR005397 510 521 PR01572 Neuropeptide FF receptor HMMPfam IPR000276 164 435 PF00001 GPCR ProfileScan IPR017452 164 435 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 146 VAAIFIISYFLIFFLCMMGNTVV 168 PRIMARY 23 2 185 FILNLAISDLLVGIFCMPITLLD 207 PRIMARY 23 3 221 KISGLVQGISVAASVFTLVAIAV 243 PRIMARY 23 4 262 AFVIIMIIWVLAITIMSPSAVML 284 PRIMARY 23 5 326 FANIYLAPLSLIVIMYGRIGISL 348 SECONDARY 23 6 373 KIIKMLLIVALLFILSWLPLWTL 395 PRIMARY 23 7 413 NIYIYPFAHWLAFGNSSVNPIIY 435 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_004876 522 607449 100.0 100.0 NP_001138228 423 607449 100.0 80.8 NP_444264 420 607449 100.0 80.5 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE9546 Download

>KIEE9546  1566 bp
ATGAATAGCTTCTTCGGAACCCCAGCGGCCAGCTGGTGCCTCCTGGAAAGTGACGTCTCA
TCTGCACCGGACAAGGAGGCGGGGAGGGAGCGCAGAGCACTCAGCGTCCAGCAGCGCGGC
GGGCCAGCCTGGAGCGGAAGCCTGGAGTGGAGCAGGCAGTCCGCGGGGGACAGACGTCGG
CTGGGATTGAGCCGGCAGACTGCGAAAAGTAGCTGGAGCCGGAGCAGGGACAGAACCTGT
TGCTGCAGACGGGCTTGGTGGATTCTGGTTCCTGCCGCCGACAGGGCTCGCCGGGAGAGG
TTCATCATGAATGAGAAATGGGACACAAACTCTTCAGAAAACTGGCATCCCATCTGGAAT
GTCAATGACACAAAGCATCATCTGTACTCAGATATTAATATTACCTATGTGAACTACTAT
CTTCACCAGCCTCAAGTGGCAGCAATCTTCATTATTTCCTACTTTCTGATCTTCTTTTTG
TGCATGATGGGAAATACTGTGGTTTGCTTTATTGTAATGAGGAACAAACATATGCACACA
GTCACTAATCTCTTCATCTTAAACCTGGCCATAAGTGATTTACTAGTTGGCATATTCTGC
ATGCCTATAACACTGCTGGACAATATTATAGCAGGATGGCCATTTGGAAACACGATGTGC
AAGATCAGTGGATTGGTCCAGGGAATATCTGTCGCAGCTTCAGTCTTTACGTTAGTTGCA
ATTGCTGTAGATAGGTTCCAGTGTGTGGTCTACCCTTTTAAACCAAAGCTCACTATCAAG
ACAGCGTTTGTCATTATTATGATCATCTGGGTCCTAGCCATCACCATTATGTCTCCATCT
GCAGTAATGTTACATGTGCAAGAAGAAAAATATTACCGAGTGAGACTCAACTCCCAGAAT
AAAACCAGTCCAGTCTACTGGTGCCGGGAAGACTGGCCAAATCAGGAAATGAGGAAGATC
TACACCACTGTGCTGTTTGCCAACATCTACCTGGCTCCCCTCTCCCTCATTGTCATCATG
TATGGAAGGATTGGAATTTCACTCTTCAGGGCTGCAGTTCCTCACACAGGCAGGAAGAAC
CAGGAGCAGTGGCACGTGGTGTCCAGGAAGAAGCAGAAGATCATTAAGATGCTCCTGATT
GTGGCCCTGCTTTTTATTCTCTCATGGCTGCCCCTGTGGACTCTAATGATGCTCTCAGAC
TACGCTGACCTTTCTCCAAATGAACTGCAGATCATCAACATCTACATCTACCCTTTTGCA
CACTGGCTGGCATTCGGCAACAGCAGTGTCAATCCCATCATTTATGGTTTCTTCAACGAG
AATTTCCGCCGTGGTTTCCAAGAAGCTTTCCAGCTCCAGCTCTGCCAAAAAAGAGCAAAG
CCTATGGAAGCTTATGCCCTAAAAGCTAAAAGCCATGTGCTCATAAACACATCTAATCAG
CTTGTCCAGGAATCTACATTTCAAAACCCTCATGGGGAAACCTTGCTTTATAGGAAAAGT
GCTGAAAAACCCCAACAGGAATTAGTGATGGAAGAATTAAAAGAAACTACTAACAGCAGT
GAGATT

ORF Protein

Cloned ORF protein sequence for pFN21AE9546 Download

>KIEE9546  522 aa
MNSFFGTPAASWCLLESDVSSAPDKEAGRERRALSVQQRGGPAWSGSLEWSRQSAGDRRR
LGLSRQTAKSSWSRSRDRTCCCRRAWWILVPAADRARRERFIMNEKWDTNSSENWHPIWN
VNDTKHHLYSDINITYVNYYLHQPQVAAIFIISYFLIFFLCMMGNTVVCFIVMRNKHMHT
VTNLFILNLAISDLLVGIFCMPITLLDNIIAGWPFGNTMCKISGLVQGISVAASVFTLVA
IAVDRFQCVVYPFKPKLTIKTAFVIIMIIWVLAITIMSPSAVMLHVQEEKYYRVRLNSQN
KTSPVYWCREDWPNQEMRKIYTTVLFANIYLAPLSLIVIMYGRIGISLFRAAVPHTGRKN
QEQWHVVSRKKQKIIKMLLIVALLFILSWLPLWTLMMLSDYADLSPNELQIINIYIYPFA
HWLAFGNSSVNPIIYGFFNENFRRGFQEAFQLQLCQKRAKPMEAYALKAKSHVLINTSNQ
LVQESTFQNPHGETLLYRKSAEKPQQELVMEELKETTNSSEI

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE9546 Download

>pFN21AE9546  6271 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAATAGCTTCTTCGGAACCCCAGCGGCCAGCTG
GTGCCTCCTGGAAAGTGACGTCTCATCTGCACCGGACAAGGAGGCGGGGAGGGAGCGCAG
AGCACTCAGCGTCCAGCAGCGCGGCGGGCCAGCCTGGAGCGGAAGCCTGGAGTGGAGCAG
GCAGTCCGCGGGGGACAGACGTCGGCTGGGATTGAGCCGGCAGACTGCGAAAAGTAGCTG
GAGCCGGAGCAGGGACAGAACCTGTTGCTGCAGACGGGCTTGGTGGATTCTGGTTCCTGC
CGCCGACAGGGCTCGCCGGGAGAGGTTCATCATGAATGAGAAATGGGACACAAACTCTTC
AGAAAACTGGCATCCCATCTGGAATGTCAATGACACAAAGCATCATCTGTACTCAGATAT
TAATATTACCTATGTGAACTACTATCTTCACCAGCCTCAAGTGGCAGCAATCTTCATTAT
TTCCTACTTTCTGATCTTCTTTTTGTGCATGATGGGAAATACTGTGGTTTGCTTTATTGT
AATGAGGAACAAACATATGCACACAGTCACTAATCTCTTCATCTTAAACCTGGCCATAAG
TGATTTACTAGTTGGCATATTCTGCATGCCTATAACACTGCTGGACAATATTATAGCAGG
ATGGCCATTTGGAAACACGATGTGCAAGATCAGTGGATTGGTCCAGGGAATATCTGTCGC
AGCTTCAGTCTTTACGTTAGTTGCAATTGCTGTAGATAGGTTCCAGTGTGTGGTCTACCC
TTTTAAACCAAAGCTCACTATCAAGACAGCGTTTGTCATTATTATGATCATCTGGGTCCT
AGCCATCACCATTATGTCTCCATCTGCAGTAATGTTACATGTGCAAGAAGAAAAATATTA
CCGAGTGAGACTCAACTCCCAGAATAAAACCAGTCCAGTCTACTGGTGCCGGGAAGACTG
GCCAAATCAGGAAATGAGGAAGATCTACACCACTGTGCTGTTTGCCAACATCTACCTGGC
TCCCCTCTCCCTCATTGTCATCATGTATGGAAGGATTGGAATTTCACTCTTCAGGGCTGC
AGTTCCTCACACAGGCAGGAAGAACCAGGAGCAGTGGCACGTGGTGTCCAGGAAGAAGCA
GAAGATCATTAAGATGCTCCTGATTGTGGCCCTGCTTTTTATTCTCTCATGGCTGCCCCT
GTGGACTCTAATGATGCTCTCAGACTACGCTGACCTTTCTCCAAATGAACTGCAGATCAT
CAACATCTACATCTACCCTTTTGCACACTGGCTGGCATTCGGCAACAGCAGTGTCAATCC
CATCATTTATGGTTTCTTCAACGAGAATTTCCGCCGTGGTTTCCAAGAAGCTTTCCAGCT
CCAGCTCTGCCAAAAAAGAGCAAAGCCTATGGAAGCTTATGCCCTAAAAGCTAAAAGCCA
TGTGCTCATAAACACATCTAATCAGCTTGTCCAGGAATCTACATTTCAAAACCCTCATGG
GGAAACCTTGCTTTATAGGAAAAGTGCTGAAAAACCCCAACAGGAATTAGTGATGGAAGA
ATTAAAAGAAACTACTAACAGCAGTGAGATTGTTTAAACGAATTCGGGCTCGGTACCCGG
GGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAA
AGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGG
CCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATG
CAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATT
ATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAG
GGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAA
TTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACA
TTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACG
TCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATA
CATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGA
AAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCA
TTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGAT
CAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAG
AGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGC
GCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCT
CAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACA
GTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTT
CTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCAT
GTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGT
GACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTA
CTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGA
CCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGT
GAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATC
GTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCT
GAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCC
AACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACG
CAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGT
TGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAA
GTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCT
CCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCC
CTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGG
TCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCT
TATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAG
CAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGA
AGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGA
AGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTG
GTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAG
AAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAG
GGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAA
ATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAG
CTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATA
CGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTA
CCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTA
TGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCC
ACGATGCGTCCGGCGTAGAGGATCGAGATCT