################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbvCI BcgI BciVI BfrI BlnI BlpI Bpu1102I BsaBI BsaI BseAI BseRI BsiEI BsiWI BsoBI Bsp1407I BspDI BspEI BspMI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI FspI HaeII HincII HindII HpaI KasI KpnI MamI MluI MroI NarI NcoI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI Psp1406I PvuI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SspBI StuI StyI SwaI TaqI Tth111I TthHB8I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AfaI 384 GTAC GT^AC AflIII 852 ACATGT A^C[GA][CT]GT AlwNI 231 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 394 ATTAAT AT^TAAT AsnI 394 ATTAAT AT^TAAT Asp700I 3 GAATAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanII 284 GGGCTC G[GA]GC[CT]^C BbrPI 1093 CACGTG CAC^GTG BbsI 928 GAAGAC GAAGAC(2/6) BclI 800 TGATCA T^GATCA BglI 277 GCCGACAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 956 AGATCT A^GATCT BpuAI 928 GAAGAC GAAGAC(2/6) BsaAI 1093 CACGTG [CT]AC^GT[GA] BsaWI 66 ACCGGA [AT]^CCGG[AT] BsgI 62 CTGCAC GTGCAG(16/14) BsmBI 54 CGTCTC CGTCTC(1/5) BsmI 1270 GCATTC GAATGC(1/-1) BspHI 305 TCATGA T^CATGA BspLU11I 852 ACATGT A^CATGT BsrBI 132 GAGCGG CCGCTC(-3/-3) BsrFI 191 GCCGGC [GA]^CCGG[CT] BstAPI 590 GCATATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 910 CCAGTCTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 956 AGATCT [GA]^GATC[CT] Cfr10I 191 GCCGGC [GA]^CCGG[CT] DraI 757 TTTAAA TTT^AAA DraII 809 GGGTCCT [GA]G^G[ACGT]CC[CT] EcoNI 1060 CCTCACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 809 GGGTCCT [GA]G^G[ACGT]CC[CT] FbaI 800 TGATCA T^GATCA HgaI 104 GCGTC GACGC(5/10) KspI 161 CCGCGG CCGC^GG MfeI 719 CAATTG C^AATTG MflI 956 AGATCT [GA]^GATC[CT] MunI 719 CAATTG C^AATTG NaeI 191 GCCGGC GCC^GGC NdeI 529 CATATG CA^TATG NgoMIV 191 GCCGGC G^CCGGC PflMI 738 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1093 CACGTG CAC^GTG PmlI 1093 CACGTG CAC^GTG PpuMI 809 GGGTCCT [GA]G^G[AT]CC[CT] PshAI 169 GACAGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 394 ATTAAT AT^TAAT PvuII 30 CAGCTG CAG^CTG RcaI 305 TCATGA T^CATGA RsaI 384 GTAC GT^AC SacII 161 CCGCGG CCGC^GG SmlI 431 CTCAAG C^T[CT][GA]AG SpeI 582 ACTAGT A^CTAGT SphI 599 GCATGC GCATG^C Van91I 738 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 956 AGATCT [GA]^GATC[CT] XmnI 3 GAATAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AatII 52 GACGTC GACGT^C 173 GACGTC AccI 748 GTCTAC GT^[AC][GT]AC 913 GTCTAC AccII 115 CGCG CG^CG 162 CGCG AcsI 1034 GAATTT [GA]^AATT[CT] 1320 GAATTT AcyI 52 GACGTC G[GA]^CG[CT]C 173 GACGTC ApoI 1034 GAATTT [GA]^AATT[CT] 1320 GAATTT AspHI 95 GAGCAC G[AT]GC[AT]^C 1417 GTGCTC AvaII 675 GGTCC G^G[AT]CC 810 GGTCC BanI 35 GGTGCC G^G[CT][GA]CC 920 GGTGCC BcnI 291 CCGGG CC^[GC]GG 924 CCGGG BfaI 583 CTAG C^TAG 814 CTAG BsaHI 52 GACGTC G[GA]^CG[CT]C 173 GACGTC BsiHKAI 95 GAGCAC G[AT]GC[AT]^C 1417 GTGCTC BstF5I 346 CATCC GGATG(2/0) 634 GGATG BstUI 115 CGCG CG^CG 162 CGCG CfoI 90 GCGC GCG^C 114 GCGC DraIII 651 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG 1093 CACGTGGTG DsaI 161 CCGCGG C^C[GA][CT]GG 1329 CCGTGG FokI 346 CATCC GGATG(9/13) 634 GGATG HhaI 90 GCGC GCG^C 114 GCGC Hin1I 52 GACGTC G[GA]^CG[CT]C 173 GACGTC HinP1I 90 GCGC G^CGC 114 GCGC HindIII 1343 AAGCTT A^AGCTT 1388 AAGCTT HphI 423 TCACC GGTGA(8/7) 821 TCACC MaeI 583 CTAG C^TAG 814 CTAG MvnI 115 CGCG CG^CG 162 CGCG NciI 291 CCGGG CC^[GC]GG 924 CCGGG NspI 599 GCATGC [GA]CATG^[CT] 852 ACATGT PleI 884 GACTC GAGTC(4/5) 1179 GACTC TfiI 261 GATTC G^A[AT]TC 1450 GAATC Tsp45I 50 GTGAC ^GT[GC]AC 541 GTCAC XcmI 22 CCAGCGGCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 980 CCAACATCTACCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AciI AluI BalI BbvI BmyI BpmI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII EaeI EarI EcoRII FauI Fnu4HI HaeIII HapII HinfI HpaII ItaI Ksp632I MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PstI Sau3AI Sau96I ScrFI SfaNI SfcI SspI Tru9I TseI Tsp509I TspRI