(Circular) (Six-base) MAPSORT of: pFN21AE9546.seq Check: 5375 from: 1 to: 6271 pFN21AE9546 6271 bp With 182 enzymes: SgfI * December 28, 2011 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2061 2182 4081 278 Size: 53 83 186 936 525 121 1899 2468 Fragments arranged by size: 2468 1899 936 525 186 121 83 53 AccI GT'mk_AC Cuts at: 1937 2754 2919 3614 1937 Size: 817 165 695 4594 Fragments arranged by size: 4594 817 695 165 Acc65I G'GTAC_C Cuts at: 3592 3592 Size: 6271 AclI AA'CG_TT Cuts at: 1563 4398 4771 1563 Size: 2835 373 3063 Fragments arranged by size: 3063 2835 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2322 2688 3034 4334 5718 5986 1632 Size: 690 366 346 1300 1384 268 1917 Fragments arranged by size: 1917 1384 1300 690 366 346 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6074 AflIII A'CryG_T Cuts at: 1203 2857 5170 6147 1203 Size: 1654 2313 977 1327 Fragments arranged by size: 2313 1654 1327 977 AgeI A'CCGG_T Cuts at: 5102 5102 Size: 6271 AhdI GACnn_n'nnGTC Cuts at: 5000 5000 Size: 6271 AleI CACnn'nnGTG Cuts at: 1166 2972 1166 Size: 1806 4465 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6271 AlwNI CAG_nnn'CTG Cuts at: 2241 4014 5586 2241 Size: 1773 1572 2926 Fragments arranged by size: 2926 1773 1572 ApaLI G'TGCA_C Cuts at: 4328 5484 4328 Size: 1156 5115 ApoI r'AATT_y Cuts at: 1493 3039 3325 3580 3806 3958 6067 1493 Size: 1546 286 255 226 152 2109 1697 Fragments arranged by size: 2109 1697 1546 286 255 226 152 AseI AT'TA_AT Cuts at: 160 1032 2400 4825 160 Size: 872 1368 2425 1606 Fragments arranged by size: 2425 1606 1368 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6271 AvaI C'yCGr_G Cuts at: 1870 1943 3596 1870 Size: 73 1653 4545 Fragments arranged by size: 4545 1653 73 BamHI G'GATC_C Cuts at: 3601 3968 3601 Size: 367 5904 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2040 2925 3592 5047 618 Size: 325 217 627 253 885 667 1455 1842 Fragments arranged by size: 1842 1455 885 667 627 325 253 217 BanII G_rGCy'C Cuts at: 1391 2293 3590 1391 Size: 902 1297 4072 Fragments arranged by size: 4072 1297 902 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2940 928 Size: 971 1041 4259 Fragments arranged by size: 4259 1041 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4462 4496 1903 Size: 34 2525 34 3678 Fragments arranged by size: 3678 2525 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4163 5373 4163 Size: 1210 5061 BclI T'GATC_A Cuts at: 1548 2805 1548 Size: 1257 5014 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2288 4882 136 Size: 107 122 71 631 605 616 2594 1525 Fragments arranged by size: 2594 1525 631 616 605 122 107 71 BglII A'GATC_T Cuts at: 2961 6266 2961 Size: 3305 2966 BlpI GC'TnA_GC Cuts at: 3688 3688 Size: 6271 Bme1580I G_kGCm'C Cuts at: 1790 4332 5488 1790 Size: 2542 1156 2573 Fragments arranged by size: 2573 2542 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6271 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4002 4960 442 Size: 416 53 83 399 24 2585 958 1753 Fragments arranged by size: 2585 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6271 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2906 2906 Size: 6271 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2155 2168 2239 3344 4931 1239 Size: 916 13 71 1105 1587 2579 Fragments arranged by size: 2579 1587 1105 916 71 13 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2421 4396 5261 5559 2421 Size: 1975 865 298 3133 Fragments arranged by size: 3133 1975 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4934 882 Size: 617 3435 2219 Fragments arranged by size: 3435 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3100 4062 493 Size: 711 1896 962 2702 Fragments arranged by size: 2702 1896 962 711 BsaBI GATnn'nnATC Cuts at: 6265 6265 Size: 6271 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2058 2179 4078 4460 Size: 53 83 186 936 525 121 1899 382 Cuts at: 4460 5095 275 Size: 635 1451 Fragments arranged by size: 1899 1451 936 635 525 382 186 121 83 53 BsaWI w'CCGG_w Cuts at: 2071 4704 5102 5376 5523 5934 2071 Size: 2633 398 274 147 411 2408 Fragments arranged by size: 2633 2408 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6046 Fragments arranged by size: 6046 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6271 BseYI C'CCAG_C Cuts at: 2026 2185 5474 5970 2026 Size: 159 3289 496 2327 Fragments arranged by size: 3289 2327 496 159 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2052 1163 Size: 573 316 5382 Fragments arranged by size: 5382 573 316 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3721 4482 5510 1540 Size: 275 186 1720 761 1028 2301 Fragments arranged by size: 2301 1720 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2104 3426 4332 4417 5488 2104 Size: 1322 906 85 1071 2887 Fragments arranged by size: 2887 1322 1071 906 85 BsmI GAATG_Cn' Cuts at: 3275 3782 3875 3275 Size: 507 93 5671 Fragments arranged by size: 5671 507 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2065 2065 Size: 6271 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2104 2293 3426 3590 4332 4417 5488 Size: 399 314 189 1133 164 742 85 1071 Cuts at: 5488 1391 Size: 2174 Fragments arranged by size: 2174 1133 1071 742 399 314 189 164 85 BspEI T'CCGG_A Cuts at: 5934 5934 Size: 6271 BspHI T'CATG_A Cuts at: 2310 4160 5890 2310 Size: 1850 1730 2691 Fragments arranged by size: 2691 1850 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3626 844 Size: 2782 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2139 4158 1394 Size: 429 316 2019 3507 Fragments arranged by size: 3507 2019 429 316 BsrDI GCAATG_nn' Cuts at: 65 1248 4766 4940 65 Size: 1183 3518 174 1396 Fragments arranged by size: 3518 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2196 4915 5102 1700 Size: 110 386 2719 187 2869 Fragments arranged by size: 2869 2719 386 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6271 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6271 BssSI C'ACGA_G Cuts at: 4331 5343 4331 Size: 1012 5259 BstAPI GCAn_nnn'nTGC Cuts at: 794 2601 794 Size: 1807 4464 BstBI TT'CG_AA Cuts at: 5075 5075 Size: 6271 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6271 BstXI CCAn_nnnn'nTGG Cuts at: 2922 2922 Size: 6271 BstYI r'GATC_y Cuts at: 1972 2961 3601 3968 4353 4370 5822 5908 5920 Size: 989 640 367 385 17 1452 86 12 Cuts at: 5920 6266 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 989 640 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2166 3334 513 Size: 552 1101 1168 3450 Fragments arranged by size: 3450 1168 1101 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5532 Fragments arranged by size: 5532 683 56 BtsI GCAGTG_nn' Cuts at: 2615 3098 3295 3568 3787 4581 4601 2615 Size: 483 197 273 219 794 20 4285 Fragments arranged by size: 4285 794 483 273 219 197 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6271 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2653 2688 529 Size: 35 2089 35 4112 Fragments arranged by size: 4112 2089 35 35 DraI TTT'AAA Cuts at: 2764 3575 2764 Size: 811 5460 DraIII CAC_nnn'GTG Cuts at: 2661 3103 2661 Size: 442 5829 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5278 809 Size: 507 3962 1802 Fragments arranged by size: 3962 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2031 2571 2642 Size: 54 1425 50 275 18 201 540 71 Cuts at: 2642 2939 3718 4607 6236 8 Size: 297 779 889 1629 43 Fragments arranged by size: 1629 1425 889 779 540 297 275 201 71 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3718 1537 Size: 275 1906 4090 Fragments arranged by size: 4090 1906 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2341 2561 3053 4201 1353 Size: 988 220 492 1148 3423 Fragments arranged by size: 3423 1148 988 492 220 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3320 4858 5232 5378 387 Size: 1064 138 1731 1538 374 146 1280 Fragments arranged by size: 1731 1538 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2155 2168 2239 2322 2688 3034 3344 Size: 393 523 13 71 83 366 346 310 Cuts at: 3344 4334 4931 5718 5986 1239 Size: 990 597 787 268 1524 Fragments arranged by size: 1524 990 787 597 523 393 366 346 310 268 83 71 13 EcoNI CCTnn'n_nnAGG Cuts at: 3069 3069 Size: 6271 EcoO109I rG'GnC_Cy Cuts at: 2815 2815 Size: 6271 EcoRI G'AATT_C Cuts at: 3580 3958 3580 Size: 378 5893 FspI TGC'GCA Cuts at: 4777 4777 Size: 6271 HaeII r_GCGC'y Cuts at: 1678 5418 1678 Size: 3740 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2048 2080 2318 2350 4912 Size: 32 135 32 476 32 238 32 2562 Cuts at: 4912 4944 4986 5018 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2562 476 238 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3615 3861 669 Size: 988 281 1677 246 3079 Fragments arranged by size: 3079 1677 988 281 246 HindIII A'AGCT_T Cuts at: 748 3348 3393 748 Size: 2600 45 3626 Fragments arranged by size: 3626 2600 45 HpaI GTT'AAC Cuts at: 3861 3861 Size: 6271 KpnI G_GTAC'C Cuts at: 3596 3596 Size: 6271 MfeI C'AATT_G Cuts at: 2724 3870 2724 Size: 1146 5125 MluI A'CGCG_T Cuts at: 6147 6147 Size: 6271 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5385 5569 1305 Size: 4080 184 2007 Fragments arranged by size: 4080 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2573 2644 2941 10 Size: 54 1425 343 741 71 297 3340 Fragments arranged by size: 3340 1425 741 343 297 71 54 MslI CAynn'nnrTG Cuts at: 518 1166 2801 2831 2972 3131 4229 4588 4747 Size: 648 1635 30 141 159 1098 359 159 Cuts at: 4747 518 Size: 2042 Fragments arranged by size: 2042 1635 1098 648 359 159 159 141 30 MspA1I CmG'CkG Cuts at: 1606 2030 2037 2168 3687 4364 5512 5757 1606 Size: 424 7 131 1519 677 1148 245 2120 Fragments arranged by size: 2120 1519 1148 677 424 245 131 7 NaeI GCC'GGC Cuts at: 2198 2198 Size: 6271 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5719 NdeI CA'TA_TG Cuts at: 387 2535 387 Size: 2148 4123 NgoMIV G'CCGG_C Cuts at: 2196 2196 Size: 6271 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6271 NotI GC'GGCC_GC Cuts at: 3718 3718 Size: 6271 NspI r_CATG'y Cuts at: 1132 1229 2608 2861 3629 5174 1132 Size: 97 1379 253 768 1545 2229 Fragments arranged by size: 2229 1545 1379 768 253 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6271 PciI A'CATG_T Cuts at: 2857 5170 2857 Size: 2313 3958 PflMI CCAn_nnn'nTGG Cuts at: 1108 2749 1108 Size: 1641 4630 PfoI T'CCnGG_A Cuts at: 2046 3107 3450 2046 Size: 1061 343 4867 Fragments arranged by size: 4867 1061 343 PmeI GTTT'AAAC Cuts at: 3575 3575 Size: 6271 PmlI CAC'GTG Cuts at: 3100 3100 Size: 6271 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4340 4372 5853 5885 4340 Size: 32 1481 32 4726 Fragments arranged by size: 4726 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2815 2815 Size: 6271 PshAI GACnn'nnGTC Cuts at: 1613 2178 1613 Size: 565 5706 PsiI TTA'TAA Cuts at: 3841 3841 Size: 6271 PstI C_TGCA'G Cuts at: 830 2252 2848 3061 3234 3623 830 Size: 1422 596 213 173 389 3478 Fragments arranged by size: 3478 1422 596 389 213 173 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6271 PvuII CAG'CTG Cuts at: 2037 2037 Size: 6271 SacII CC_GC'GG Cuts at: 2169 2169 Size: 6271 SalI G'TCGA_C Cuts at: 1936 3613 1936 Size: 1677 4594 SbfI CC_TGCA'GG Cuts at: 3623 3623 Size: 6271 ScaI AGT'ACT Cuts at: 4519 6231 4519 Size: 1712 4559 SfcI C'TryA_G Cuts at: 826 1045 2248 2730 2844 3057 3230 3619 4754 Size: 219 1203 482 114 213 173 389 1135 Cuts at: 4754 5435 5626 826 Size: 681 191 1471 Fragments arranged by size: 1471 1203 1135 681 482 389 219 213 191 173 114 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6271 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6271 SmaI CCC'GGG Cuts at: 1872 3598 1872 Size: 1726 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2436 4375 5276 5538 820 Size: 197 926 493 1939 901 262 1553 Fragments arranged by size: 1939 1553 926 901 493 262 197 SnaBI TAC'GTA Cuts at: 493 4062 493 Size: 3569 2702 SpeI A'CTAG_T Cuts at: 152 2587 152 Size: 2435 3836 SphI G_CATG'C Cuts at: 1132 2608 3629 1132 Size: 1476 1021 3774 Fragments arranged by size: 3774 1476 1021 SspI AAT'ATT Cuts at: 5 52 2404 2629 2876 4195 5 Size: 47 2352 225 247 1319 2081 Fragments arranged by size: 2352 2081 1319 247 225 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6271 StyI C'CwwG_G Cuts at: 513 1065 3710 513 Size: 552 2645 3074 Fragments arranged by size: 3074 2645 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4298 4315 4468 4653 5098 325 Size: 1255 2718 17 153 185 445 1498 Fragments arranged by size: 2718 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2388 4517 6229 Size: 274 80 33 51 1443 411 2129 1712 Cuts at: 6229 96 Size: 138 Fragments arranged by size: 2129 1712 1443 411 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2094 2126 2487 2519 3089 3121 6231 6263 2094 Size: 32 361 32 570 32 3110 32 2102 Fragments arranged by size: 3110 2102 570 361 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6271 XbaI T'CTAG_A Cuts at: 3607 3607 Size: 6271 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2034 2992 2034 Size: 958 5313 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6271 XmaI C'CCGG_G Cuts at: 1870 3596 1870 Size: 1726 4545 XmnI GAAnn'nnTTC Cuts at: 2012 4400 2012 Size: 2388 3883 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2059 2180 4079 276 Size: 53 83 186 936 525 121 1899 2468 Fragments arranged by size: 2468 1899 936 525 186 121 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI