CALCRL ORF clone pFN21AE9523

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE9523

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Clone info.

Species

human

Clone Name

pFN21AE9523 Sequence (with vector) Restriction Map

Product ID

FHC21247

ORF Sequence

DNA sequence (1383 bp)
Protein sequence (461 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE9523

Symbol

CALCRL

Alias : CGRPR, CRLR, LMPHM8

Description

calcitonin receptor-like, transcript variant 1

Original Clone ID

cp99523

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1383 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 461 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS2293.1 7.7e-203 100.0 CALCRL CCDS5631.1 9.5e-118 57.8 CALCR CCDS55125.1 8e-74 55.7 CALCR CCDS56478.1 1.1e-41 33.0 CRHR2 CCDS5429.1 1.2e-41 34.6 CRHR2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR003289 3 26 PR01351 GPCR IPR003289 34 47 PR01351 GPCR IPR003287 45 64 PR01350 GPCR IPR003289 50 61 PR01351 GPCR IPR003287 75 86 PR01350 GPCR IPR003289 76 86 PR01351 GPCR IPR003287 90 104 PR01350 GPCR IPR003287 111 125 PR01350 GPCR IPR003287 128 148 PR01350 GPCR IPR000832 143 167 PR00249 GPCR IPR000832 175 199 PR00249 GPCR IPR003289 196 208 PR01351 GPCR IPR000832 214 237 PR00249 GPCR IPR000832 252 277 PR00249 GPCR IPR003287 263 281 PR01350 GPCR IPR000832 292 317 PR00249 GPCR IPR000832 334 354 PR00249 GPCR IPR003287 354 374 PR01350 GPCR IPR000832 366 387 PR00249 GPCR IPR003287 392 406 PR01350 GPCR IPR003289 405 421 PR01351 GPCR IPR003289 421 437 PR01351 GPCR IPR003287 434 444 PR01350 GPCR HMMPfam IPR001879 63 129 PF02793 GPCR IPR000832 140 380 PF00002 GPCR HMMSmart IPR001879 61 136 SM00008 GPCR ProfileScan IPR001879 47 131 PS50227 GPCR IPR017981 141 388 PS50261 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 5 CTLYFLVLLPFFMILVTAELEES 27 PRIMARY 23 2 142 FYLTIIGHGLSIASLLISLGIFF 164 PRIMARY 23 3 182 FSFVCNSVVTIIHLTAVANNQAL 204 SECONDARY 23 4 228 FWMLCEGIYLHTLIVVAVFAEKQ 250 PRIMARY 23 5 255 YYFLGWGFPLIPACIHAIARSLY 277 SECONDARY 23 6 296 GPICAALLVNLFFLLNIVRVLIT 318 PRIMARY 23 7 333 KAVRATLILVPLLGIEFVLIPWR 355 SECONDARY 23 8 368 IMHILMHFQGLLVSTIFCFFNGE 390 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001258680 461 114190 100.0 100.0 NP_005786 461 114190 100.0 100.0 XP_005246289 472 114190 100.0 100.0 XP_005246288 472 114190 100.0 100.0 XP_005246291 472 114190 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE9523 Download

>KIEE9523  1383 bp
ATGGAGAAAAAGTGTACCCTGTATTTTCTGGTTCTCTTGCCTTTTTTTATGATTCTTGTT
ACAGCAGAATTAGAAGAGAGTCCTGAGGACTCAATTCAGTTGGGAGTTACTAGAAATAAA
ATCATGACAGCTCAATATGAATGTTACCAAAAGATTATGCAAGACCCCATTCAACAAGCA
GAAGGCGTTTACTGCAACAGAACCTGGGATGGATGGCTCTGCTGGAACGATGTTGCAGCA
GGAACTGAATCAATGCAGCTCTGCCCTGATTACTTTCAGGACTTTGATCCATCAGAAAAA
GTTACAAAGATCTGTGACCAAGATGGAAACTGGTTTAGACATCCAGCAAGCAACAGAACA
TGGACAAATTATACCCAGTGTAATGTTAACACCCACGAGAAAGTGAAGACTGCACTAAAT
TTGTTTTACCTGACCATAATTGGACACGGATTGTCTATTGCATCACTGCTTATCTCGCTT
GGCATATTCTTTTATTTCAAGAGCCTAAGTTGCCAAAGGATTACCTTACACAAAAATCTG
TTCTTCTCATTTGTTTGTAACTCTGTTGTAACAATCATTCACCTCACTGCAGTGGCCAAC
AACCAGGCCTTAGTAGCCACAAATCCTGTTAGTTGCAAAGTGTCCCAGTTCATTCATCTT
TACCTGATGGGCTGTAATTACTTTTGGATGCTCTGTGAAGGCATTTACCTACACACACTC
ATTGTGGTGGCCGTGTTTGCAGAGAAGCAACATTTAATGTGGTATTATTTTCTTGGCTGG
GGATTTCCACTGATTCCTGCTTGTATACATGCCATTGCTAGAAGCTTATATTACAATGAC
AATTGCTGGATCAGTTCTGATACCCATCTCCTCTACATTATCCATGGCCCAATTTGTGCT
GCTTTACTGGTGAATCTTTTTTTCTTGTTAAATATTGTACGCGTTCTCATCACCAAGTTA
AAAGTTACACACCAAGCGGAATCCAATCTGTACATGAAAGCTGTGAGAGCTACTCTTATC
TTGGTGCCATTGCTTGGCATTGAATTTGTGCTGATTCCATGGCGACCTGAAGGAAAGATT
GCAGAGGAGGTATATGACTACATCATGCACATCCTTATGCACTTCCAGGGTCTTTTGGTC
TCTACCATTTTCTGCTTCTTTAATGGAGAGGTTCAAGCAATTCTGAGAAGAAACTGGAAT
CAATACAAAATCCAATTTGGAAACAGCTTTTCCAACTCAGAAGCTCTTCGTAGTGCGTCT
TACACAGTGTCAACAATCAGTGATGGTCCAGGTTATAGTCATGACTGTCCTAGTGAACAC
TTAAATGGAAAAAGCATCCATGATATTGAAAATGTTCTCTTAAAACCAGAAAATTTATAT
AAT

ORF Protein

Cloned ORF protein sequence for pFN21AE9523 Download

>KIEE9523  461 aa
MEKKCTLYFLVLLPFFMILVTAELEESPEDSIQLGVTRNKIMTAQYECYQKIMQDPIQQA
EGVYCNRTWDGWLCWNDVAAGTESMQLCPDYFQDFDPSEKVTKICDQDGNWFRHPASNRT
WTNYTQCNVNTHEKVKTALNLFYLTIIGHGLSIASLLISLGIFFYFKSLSCQRITLHKNL
FFSFVCNSVVTIIHLTAVANNQALVATNPVSCKVSQFIHLYLMGCNYFWMLCEGIYLHTL
IVVAVFAEKQHLMWYYFLGWGFPLIPACIHAIARSLYYNDNCWISSDTHLLYIIHGPICA
ALLVNLFFLLNIVRVLITKLKVTHQAESNLYMKAVRATLILVPLLGIEFVLIPWRPEGKI
AEEVYDYIMHILMHFQGLLVSTIFCFFNGEVQAILRRNWNQYKIQFGNSFSNSEALRSAS
YTVSTISDGPGYSHDCPSEHLNGKSIHDIENVLLKPENLYN

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE9523 Download

>pFN21AE9523  6088 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGAGAAAAAGTGTACCCTGTATTTTCTGGTTCT
CTTGCCTTTTTTTATGATTCTTGTTACAGCAGAATTAGAAGAGAGTCCTGAGGACTCAAT
TCAGTTGGGAGTTACTAGAAATAAAATCATGACAGCTCAATATGAATGTTACCAAAAGAT
TATGCAAGACCCCATTCAACAAGCAGAAGGCGTTTACTGCAACAGAACCTGGGATGGATG
GCTCTGCTGGAACGATGTTGCAGCAGGAACTGAATCAATGCAGCTCTGCCCTGATTACTT
TCAGGACTTTGATCCATCAGAAAAAGTTACAAAGATCTGTGACCAAGATGGAAACTGGTT
TAGACATCCAGCAAGCAACAGAACATGGACAAATTATACCCAGTGTAATGTTAACACCCA
CGAGAAAGTGAAGACTGCACTAAATTTGTTTTACCTGACCATAATTGGACACGGATTGTC
TATTGCATCACTGCTTATCTCGCTTGGCATATTCTTTTATTTCAAGAGCCTAAGTTGCCA
AAGGATTACCTTACACAAAAATCTGTTCTTCTCATTTGTTTGTAACTCTGTTGTAACAAT
CATTCACCTCACTGCAGTGGCCAACAACCAGGCCTTAGTAGCCACAAATCCTGTTAGTTG
CAAAGTGTCCCAGTTCATTCATCTTTACCTGATGGGCTGTAATTACTTTTGGATGCTCTG
TGAAGGCATTTACCTACACACACTCATTGTGGTGGCCGTGTTTGCAGAGAAGCAACATTT
AATGTGGTATTATTTTCTTGGCTGGGGATTTCCACTGATTCCTGCTTGTATACATGCCAT
TGCTAGAAGCTTATATTACAATGACAATTGCTGGATCAGTTCTGATACCCATCTCCTCTA
CATTATCCATGGCCCAATTTGTGCTGCTTTACTGGTGAATCTTTTTTTCTTGTTAAATAT
TGTACGCGTTCTCATCACCAAGTTAAAAGTTACACACCAAGCGGAATCCAATCTGTACAT
GAAAGCTGTGAGAGCTACTCTTATCTTGGTGCCATTGCTTGGCATTGAATTTGTGCTGAT
TCCATGGCGACCTGAAGGAAAGATTGCAGAGGAGGTATATGACTACATCATGCACATCCT
TATGCACTTCCAGGGTCTTTTGGTCTCTACCATTTTCTGCTTCTTTAATGGAGAGGTTCA
AGCAATTCTGAGAAGAAACTGGAATCAATACAAAATCCAATTTGGAAACAGCTTTTCCAA
CTCAGAAGCTCTTCGTAGTGCGTCTTACACAGTGTCAACAATCAGTGATGGTCCAGGTTA
TAGTCATGACTGTCCTAGTGAACACTTAAATGGAAAAAGCATCCATGATATTGAAAATGT
TCTCTTAAAACCAGAAAATTTATATAATGTTTAAACGAATTCGGGCTCGGTACCCGGGGA
TCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGG
AAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCG
CTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAG
TGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATA
AGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGG
GAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTC
TAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTC
ATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCA
GGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACAT
TCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAA
AGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTT
TGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAG
TTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGT
TTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCG
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAG
AATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTA
AGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTG
ACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTA
ACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGAC
ACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTT
ACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCA
CTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAG
CGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTA
GTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAG
ATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAAC
CGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAG
GAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGC
TGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTC
AGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCC
TCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTT
CGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCG
TTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTAT
CCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAG
CCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGT
GGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGC
CAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTA
GCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAG
ATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGA
TTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATA
CAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTT
CAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGG
TTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCT
GTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGG
TAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACG
ATGCGTCCGGCGTAGAGGATCGAGATCT