Restriction map for
pFN21AE9523
(Circular) (Six-base) MAPSORT of: pFN21AE9523.seq Check: 6544 from: 1 to: 6088
pFN21AE9523 6088 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3898 278
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2809 3431 1937
Size: 872 622 4594
Fragments arranged by size:
4594 872 622
Acc65I G'GTAC_C
Cuts at: 3409 3409
Size: 6088
AclI AA'CG_TT
Cuts at: 1563 4215 4588 1563
Size: 2652 373 3063
Fragments arranged by size:
3063 2652 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3093 4151 5535 5803 1632
Size: 1461 1058 1384 268 1917
Fragments arranged by size:
1917 1461 1384 1058 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5891
AflIII A'CryG_T
Cuts at: 1203 2944 4987 5964 1203
Size: 1741 2043 977 1327
Fragments arranged by size:
2043 1741 1327 977
AgeI A'CCGG_T
Cuts at: 4919 4919
Size: 6088
AhdI GACnn_n'nnGTC
Cuts at: 4817 4817
Size: 6088
AleI CACnn'nnGTG
Cuts at: 1166 2594 1166
Size: 1428 4660
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6088
AlwNI CAG_nnn'CTG
Cuts at: 2208 2249 3831 5403 2208
Size: 41 1582 1572 2893
Fragments arranged by size:
2893 1582 1572 41
ApaLI G'TGCA_C
Cuts at: 4145 5301 4145
Size: 1156 4932
ApoI r'AATT_y
Cuts at: 1493 2422 3047 3376 3397 3623 3775 5884 1493
Size: 929 625 329 21 226 152 2109 1697
Fragments arranged by size:
2109 1697 929 625 329 226 152 21
AseI AT'TA_AT
Cuts at: 160 1032 4642 160
Size: 872 3610 1606
Fragments arranged by size:
3610 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
AvaI C'yCGr_G
Cuts at: 1870 1943 3413 1870
Size: 73 1470 4545
Fragments arranged by size:
4545 1470 73
BamHI G'GATC_C
Cuts at: 3418 3785 3418
Size: 367 5721
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3028 3409 4864 618
Size: 325 217 627 1241 381 1455 1842
Fragments arranged by size:
1842 1455 1241 627 381 325 217
BanII G_rGCy'C
Cuts at: 1391 3407 1391
Size: 2016 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2417 928
Size: 971 518 4599
Fragments arranged by size:
4599 971 518
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4279 4313 1903
Size: 34 2342 34 3678
Fragments arranged by size:
3678 2342 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3980 5190 3980
Size: 1210 4878
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6088
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4699 136
Size: 107 122 71 631 605 3027 1525
Fragments arranged by size:
3027 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2313 6083 2313
Size: 3770 2318
BlpI GC'TnA_GC
Cuts at: 3505 3505
Size: 6088
Bme1580I G_kGCm'C
Cuts at: 1790 4149 5305 1790
Size: 2359 1156 2573
Fragments arranged by size:
2573 2359 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6088
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2374 2644 3819
Size: 416 53 83 399 24 957 270 1175
Cuts at: 3819 4777 442
Size: 958 1753
Fragments arranged by size:
1753 1175 958 957 416 399 270 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6088
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3033 3033
Size: 6088
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4748 1239
Size: 3509 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4213 5078 5376 4213
Size: 865 298 4925
Fragments arranged by size:
4925 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3148 4751 882
Size: 617 1649 1603 2219
Fragments arranged by size:
2219 1649 1603 617
BsaAI yAC'GTr
Cuts at: 493 1204 3879 493
Size: 711 2675 2702
Fragments arranged by size:
2702 2675 711
BsaBI GATnn'nnATC
Cuts at: 2868 6082 2868
Size: 3214 2874
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3895 4277 4912 275
Size: 53 83 186 936 2362 382 635 1451
Fragments arranged by size:
2362 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4521 4919 5193 5340 5751 4521
Size: 398 274 147 411 4858
Fragments arranged by size:
4858 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2000 2030 1178
Size: 30 165 30 597 30 5236
Fragments arranged by size:
5236 597 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2864 3104 1186
Size: 1678 240 4170
Fragments arranged by size:
4170 1678 240
BseYI C'CCAG_C
Cuts at: 2781 5291 5787 2781
Size: 2510 496 3082
Fragments arranged by size:
3082 2510 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2400 1163
Size: 573 664 4851
Fragments arranged by size:
4851 664 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3538 4299 5327 1540
Size: 275 186 1537 761 1028 2301
Fragments arranged by size:
2301 1537 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4149 4234 5305 4149
Size: 85 1071 4932
Fragments arranged by size:
4932 1071 85
BsmI GAATG_Cn'
Cuts at: 3599 3692 3599
Size: 93 5995
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3407 4149 4234 5305 1391
Size: 399 1617 742 85 1071 2174
Fragments arranged by size:
2174 1617 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5751 5751
Size: 6088
BspHI T'CATG_A
Cuts at: 2127 3304 3977 5707 2127
Size: 1177 673 1730 2508
Fragments arranged by size:
2508 1730 1177 673
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3443 844
Size: 2599 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3975 1394
Size: 429 2152 3507
Fragments arranged by size:
3507 2152 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2817 3032 4583 4757 65
Size: 1183 1569 215 1551 174 1396
Fragments arranged by size:
1569 1551 1396 1183 215 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4732 4919 1700
Size: 110 2922 187 2869
Fragments arranged by size:
2922 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2994 96
Size: 2898 3190
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6088
BssSI C'ACGA_G
Cuts at: 2399 4148 5160 2399
Size: 1749 1012 3327
Fragments arranged by size:
3327 1749 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6088
BstBI TT'CG_AA
Cuts at: 4892 4892
Size: 6088
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6088
BstYI r'GATC_y
Cuts at: 1972 2313 3418 3785 4170 4187 5639 5725 5737
Size: 341 1105 367 385 17 1452 86 12
Cuts at: 5737 6083 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1105 385 367 346 341 86
17 12
BstZ17I GTA'TAC
Cuts at: 2810 2810
Size: 6088
Bsu36I CC'TnA_GG
Cuts at: 2088 2088
Size: 6088
BtgI C'CryG_G
Cuts at: 513 1065 2004 2887 3062 513
Size: 552 939 883 175 3539
Fragments arranged by size:
3539 939 883 552 175
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5349
Fragments arranged by size:
5349 683 56
BtsI GCAGTG_nn'
Cuts at: 2468 2589 2601 3604 4398 4418 2468
Size: 121 12 1003 794 20 4138
Fragments arranged by size:
4138 1003 794 121 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6088
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6053
DraI TTT'AAA
Cuts at: 3392 3392
Size: 6088
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5095 809
Size: 507 3779 1802
Fragments arranged by size:
3779 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2598 2733 3535
Size: 54 1425 50 275 18 768 135 802
Cuts at: 3535 4424 6053 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 802 768 275 135 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3535 1537
Size: 275 1723 4090
Fragments arranged by size:
4090 1723 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2073 3255 4018 1353
Size: 720 1182 763 3423
Fragments arranged by size:
3423 1182 763 720
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4675 5049 5195 387
Size: 1064 138 3086 374 146 1280
Fragments arranged by size:
3086 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3093 4151 4748 5535 5803 1239
Size: 393 1461 1058 597 787 268 1524
Fragments arranged by size:
1524 1461 1058 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3397 3775 3397
Size: 378 5710
FspI TGC'GCA
Cuts at: 4594 4594
Size: 6088
HaeII r_GCGC'y
Cuts at: 1678 5235 1678
Size: 3557 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4729 4761 4803 4835 1373
Size: 32 135 32 3157 32 42 32 2626
Fragments arranged by size:
3157 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2392 3276 3432 3678 669
Size: 988 281 454 884 156 246 3079
Fragments arranged by size:
3079 988 884 454 281 246 156
HindIII A'AGCT_T
Cuts at: 748 2827 748
Size: 2079 4009
HpaI GTT'AAC
Cuts at: 2392 3678 2392
Size: 1286 4802
KpnI G_GTAC'C
Cuts at: 3413 3413
Size: 6088
MfeI C'AATT_G
Cuts at: 2845 3687 2845
Size: 842 5246
MluI A'CGCG_T
Cuts at: 2944 5964 2944
Size: 3020 3068
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3261 5202 5386 1305
Size: 1956 1941 184 2007
Fragments arranged by size:
2007 1956 1941 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2600 10
Size: 54 1425 343 768 3498
Fragments arranged by size:
3498 1425 768 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2594 2729 3119 3327 4046 4405 4564
Size: 648 1428 135 390 208 719 359 159
Cuts at: 4564 518
Size: 2042
Fragments arranged by size:
2042 1428 719 648 390 359 208 159
135
MspA1I CmG'CkG
Cuts at: 1606 3504 4181 5329 5574 1606
Size: 1898 677 1148 245 2120
Fragments arranged by size:
2120 1898 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2887 3062 513
Size: 552 939 883 175 3539
Fragments arranged by size:
3539 939 883 552 175
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6088
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6088
NotI GC'GGCC_GC
Cuts at: 3535 3535
Size: 6088
NspI r_CATG'y
Cuts at: 1132 1229 2816 3446 4991 1132
Size: 97 1587 630 1545 2229
Fragments arranged by size:
2229 1587 1545 630 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6088
PciI A'CATG_T
Cuts at: 4987 4987
Size: 6088
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3038 1108
Size: 1930 4158
PmeI GTTT'AAAC
Cuts at: 3392 3392
Size: 6088
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4157 4189 5670 5702 4157
Size: 32 1481 32 4543
Fragments arranged by size:
4543 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6088
PsiI TTA'TAA
Cuts at: 3658 3658
Size: 6088
PstI C_TGCA'G
Cuts at: 830 2596 3440 830
Size: 1766 844 3478
Fragments arranged by size:
3478 1766 844
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6088
SalI G'TCGA_C
Cuts at: 1936 3430 1936
Size: 1494 4594
SapI GCTCTTCn'nnn_
Cuts at: 3255 3255
Size: 6088
SbfI CC_TGCA'GG
Cuts at: 3440 3440
Size: 6088
ScaI AGT'ACT
Cuts at: 4336 6048 4336
Size: 1712 4376
SfcI C'TryA_G
Cuts at: 826 1045 2592 3436 4571 5252 5443 826
Size: 219 1547 844 1135 681 191 1471
Fragments arranged by size:
1547 1471 1135 844 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6088
SmaI CCC'GGG
Cuts at: 1872 3415 1872
Size: 1543 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4192 5093 5355 820
Size: 197 926 2249 901 262 1553
Fragments arranged by size:
2249 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3879 493
Size: 3386 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6088
SphI G_CATG'C
Cuts at: 1132 3446 1132
Size: 2314 3774
SspI AAT'ATT
Cuts at: 5 52 2938 4012 5
Size: 47 2886 1074 2081
Fragments arranged by size:
2886 2081 1074 47
StuI AGG'CCT
Cuts at: 1517 2612 1517
Size: 1095 4993
StyI C'CwwG_G
Cuts at: 513 1065 2004 2887 3062 3527 513
Size: 552 939 883 175 465 3074
Fragments arranged by size:
3074 939 883 552 465 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2411 4115 4132 4285 4470 4915 325
Size: 1255 831 1704 17 153 185 445 1498
Fragments arranged by size:
1704 1498 1255 831 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2994 4334 6046
Size: 274 80 33 51 1443 1017 1340 1712
Cuts at: 6046 96
Size: 138
Fragments arranged by size:
1712 1443 1340 1017 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2000 2032 2683 2715 6048 6080 2000
Size: 32 651 32 3333 32 2008
Fragments arranged by size:
3333 2008 651 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6088
XbaI T'CTAG_A
Cuts at: 3424 3424
Size: 6088
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6088
XmaI C'CCGG_G
Cuts at: 1870 3413 1870
Size: 1543 4545
XmnI GAAnn'nnTTC
Cuts at: 3249 3357 4217 3249
Size: 108 860 5120
Fragments arranged by size:
5120 860 108
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3896 276
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI