################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BssHII BstAPI BstBI BstEII BstPI BstXI CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HhaI Hin1I HinP1I HpaII KasI KpnI KspI MaeII MroI MspA1I MspI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 803 GTATAC GT^[AC][GT]AC AccII 940 CGCG CG^CG AciI 976 GCGG CCGC(-3/-1) AflIII 939 ACGCGT A^C[GA][CT]GT AocI 82 CCTGAGG CC^T[ACGT]AGG AvaII 1285 GGTCC G^G[AT]CC BalI 593 TGGCCA TGG^CCA BanI 1023 GGTGCC G^G[CT][GA]CC BbsI 405 GAAGAC GAAGAC(2/6) BglII 308 AGATCT A^GATCT BpuAI 405 GAAGAC GAAGAC(2/6) BsaBI 859 GATACCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1137 GGTCTC GGTCTC(1/5) BsgI 410 CTGCAC GTGCAG(16/14) BsiYI 1027 CCATTGCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 1027 CCATTGCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 1138 GTCTC GTCTC(1/5) BsmFI 642 GTCCC GGGAC(10/14) Bsp1407I 989 TGTACA T^GTACA BsrGI 989 TGTACA T^GTACA BssSI 394 CACGAG CACGAG(-5/-1) Bst1107I 803 GTATAC GTA^TAC BstUI 940 CGCG CG^CG BstYI 308 AGATCT [GA]^GATC[CT] BstZ17I 803 GTATAC GTA^TAC Bsu36I 82 CCTGAGG CC^T[ACGT]AGG Cac8I 346 GCAAGC GC[ACGT]^[ACGT]GC Eco81I 82 CCTGAGG CC^T[ACGT]AGG HgaI 1255 GCGTC GACGC(5/10) HindIII 822 AAGCTT A^AGCTT HpaI 385 GTTAAC GTT^AAC MamI 859 GATACCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 840 CAATTG C^AATTG MflI 308 AGATCT [GA]^GATC[CT] MluI 939 ACGCGT A^CGCGT MluNI 593 TGGCCA TGG^CCA MscI 593 TGGCCA TGG^CCA MunI 840 CAATTG C^AATTG MvnI 940 CGCG CG^CG NlaIV 1023 GGTGCC GG[ACGT]^[ACGT]CC NspI 807 ACATGC [GA]CATG^[CT] PflMI 1027 CCATTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 587 CTGCAG CTGCA^G SapI 1243 GCTCTTC GCTCTTC(1/4) SfcI 587 CTGCAG C^T[GA][CT]AG SspBI 989 TGTACA T^GTACA SspI 931 AATATT AAT^ATT StuI 605 AGGCCT AGG^CCT Tsp45I 314 GTGAC ^GT[GC]AC Van91I 1027 CCATTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 308 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AlwI 286 GATCC GGATC(4/5) 848 GGATC AlwNI 198 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG 239 CAGGAACTG Asp700I 1240 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1348 GAAAATGTTC BseRI 868 CTCCTC GAGGAG(10/8) 1084 GAGGAG BspHI 122 TCATGA T^CATGA 1299 TCATGA BsrDI 813 CATTGC GCAATG(2/0) 1028 CATTGC Cfr13I 886 GGCCC G^G[ACGT]CC 1285 GGTCC DsaI 882 CCATGG C^C[GA][CT]GG 1057 CCATGG EaeI 593 TGGCCA [CT]^GGCC[GA] 728 TGGCCG EarI 1244 CTCTTC CTCTTC(1/4) 73 GAAGAG EcoT14I 882 CCATGG C^C[AT][AT]GG 1057 CCATGG HincII 385 GTTAAC GT[CT]^[GA]AC 1269 GTCAAC HindII 385 GTTAAC GT[CT]^[GA]AC 1269 GTCAAC Ksp632I 1244 CTCTTC CTCTTC(1/4) 73 GAAGAG NcoI 882 CCATGG C^CATGG 1057 CCATGG PleI 88 GACTC GAGTC(4/5) 78 GAGTC RcaI 122 TCATGA T^CATGA 1299 TCATGA Sau96I 886 GGCCC G^G[ACGT]CC 1285 GGTCC StyI 882 CCATGG C^C[AT][AT]GG 1057 CCATGG XmnI 1240 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1348 GAAAATGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AcsI AfaI AluI ApoI BbvI BfaI BsaJI BsrI BssKI BstF5I BstNI DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MslI MvaI MwoI NdeII NlaIII RsaI Sau3AI ScrFI SfaNI TfiI Tru9I TseI Tsp509I TspRI