SLC6A20 ORF clone pFN21AE6468

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE6468

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Clone info.

Species

human

Clone Name

pFN21AE6468 Sequence (with vector) Restriction Map

Product ID

FHC21513

ORF Sequence

DNA sequence (1776 bp)
Protein sequence (592 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE6468

Symbol

SLC6A20

Alias : IMINO, SIT1, XT3, Xtrp3

Description

solute carrier family 6 (proline IMINO transporter), member 20, transcript variant 1

Original Clone ID

oc96468

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1776 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 592 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS43077.1 0 100.0 SLC6A20 CCDS2730.1 9.1e-152 93.8 SLC6A20 CCDS3860.1 2.9e-131 48.1 SLC6A18 CCDS34130.1 3e-129 46.3 SLC6A19 CCDS9026.1 9.7e-101 44.7 SLC6A15 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000175 13 34 PR00176 Sodium:neurotransmitter symporter IPR000175 42 61 PR00176 Sodium:neurotransmitter symporter IPR000175 86 112 PR00176 Sodium:neurotransmitter symporter IPR002438 138 150 PR01206 Sodium:neurotransmitter symporter IPR002438 151 162 PR01206 Sodium:neurotransmitter symporter IPR000175 195 212 PR00176 Sodium:neurotransmitter symporter IPR000175 277 297 PR00176 Sodium:neurotransmitter symporter IPR002438 300 310 PR01206 Sodium:neurotransmitter symporter IPR000175 385 404 PR00176 Sodium:neurotransmitter symporter IPR000175 466 486 PR00176 Sodium:neurotransmitter symporter IPR000175 506 526 PR00176 Sodium:neurotransmitter symporter HMMPfam IPR000175 5 579 PF00209 Sodium:neurotransmitter symporter ProfileScan NULL 1 18 PS51257 NULL IPR000175 4 583 PS50267 Sodium:neurotransmitter symporter
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 5 RPLWANSLQFVFACISYAVGLGN 27 PRIMARY 23 2 44 LVPYIIMLIVEGMPLLYLELAVG 66 PRIMARY 23 3 81 SPYLSGVGVASVVVSFFLSMYYN 103 PRIMARY 23 4 169 EPALCLLLAWLVVYLCILRGTES 191 PRIMARY 23 5 193 GKVVYFTASLPYCVLIIYLIRGL 215 PRIMARY 23 6 242 INAATQIFFSLGLGFGSLIAFA 263 SECONDARY 22 7 277 IIVSLINSFTSIFASIVTFSIYG 299 SECONDARY 23 8 391 WSVLYFFMLLMLGIGSMLGNTAA 413 PRIMARY 23 9 431 EAISGLVCLVNCAIGMVFTMEAG 453 PRIMARY 23 10 465 ATLSLLLIVLVETIAVCYVYGL 486 PRIMARY 22 11 508 MWAGVSPLLIVSLFVFYLSDYIL 530 PRIMARY 23 12 556 LAVIGLLVASSTMCIPLAALGTF 578 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_064593 592 138500,242600,605616 100.0 100.0 XP_011532149 493 138500,242600,605616 100.0 83.3 NP_071800 555 138500,242600,605616 93.8 100.0 NP_872438 628 610300 48.1 99.0 NP_001003841 634 138500,234500,242600,608893 46.3 98.6 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE6468 Download

>KIEE6468  1776 bp
ATGGAGAAAGCGCGGCCGCTGTGGGCCAACTCGCTACAGTTCGTGTTCGCCTGCATCTCG
TACGCCGTGGGCCTGGGCAACGTGTGGCGATTCCCGTACCTGTGCCAGATGTACGGCGGA
GGTAGTTTCCTGGTCCCCTACATCATCATGCTTATCGTGGAGGGAATGCCGCTCTTGTAC
CTGGAACTGGCTGTGGGGCAGCGCATGCGGCAGGGCAGCATCGGCGCCTGGAGGACCATC
AGCCCGTACCTCAGTGGTGTCGGGGTCGCCAGCGTGGTGGTCTCTTTCTTCCTCTCCATG
TACTACAACGTCATCAACGCCTGGGCCTTCTGGTACCTCTTCCACTCCTTCCAGGATCCC
CTGCCGTGGTCTGTCTGCCCACTGAATGGTAACCACACGGGCTACGATGAGGAGTGCGAG
AAGGCGTCCTCCACACAGTACTTCTGGTACAGGAAAACCCTCAATATCTCGCCGTCCCTC
CAGGAGAACGGGGGTGTGCAGTGGGAGCCGGCGCTGTGCCTCCTCCTGGCCTGGCTGGTG
GTGTACCTGTGCATCCTGCGTGGCACCGAGTCCACTGGCAAGGTGGTGTATTTCACGGCG
TCACTGCCCTATTGCGTGCTCATCATCTACCTCATCAGGGGCCTCACGCTCCACGGAGCC
ACCAATGGCCTCATGTACATGTTCACTCCCAAGATAGAGCAGCTGGCCAACCCCAAGGCC
TGGATCAATGCAGCCACCCAGATCTTCTTCTCACTTGGCCTGGGCTTCGGCAGCCTGATC
GCCTTCGCCAGCTACAATGAGCCATCCAACAACTGCCAGAAGCACGCCATCATCGTGTCC
CTCATCAACAGCTTCACCTCCATATTTGCCAGCATTGTCACCTTCTCCATCTATGGCTTC
AAGGCCACCTTCAATTATGAAAACTGCTTGAAGAAGGTGAGTCTGCTGCTGACCAACACT
TTTGACCTTGAAGATGGCTTTTTGACAGCCAGCAACCTGGAGCAGGTGAAGGGCTACCTC
GCATCTGCCTACCCAAGCAAATACAGCGAGATGTTCCCGCAAATCAAAAACTGCAGCTTG
GAATCGGAGCTAGACACGGCCGTCCAGGGCACTGGCCTGGCATTCATCGTCTATACAGAG
GCCATTAAAAACATGGAGGTGTCCCAGCTGTGGTCGGTGCTCTACTTCTTCATGCTGCTG
ATGCTGGGCATTGGGAGCATGCTGGGGAACACAGCGGCCATCCTCACCCCTCTGACAGAC
AGCAAGATCATCTCCAGCCACCTGCCCAAGGAGGCCATCTCAGGTCTGGTGTGCCTTGTC
AACTGTGCCATTGGCATGGTGTTCACGATGGAGGCTGGGAACTACTGGTTTGACATATTC
AACGACTACGCGGCCACACTGTCCCTGCTGCTCATCGTGCTGGTGGAGACGATTGCCGTG
TGCTACGTGTACGGGCTGAGGAGATTTGAAAGTGACCTTAAGGCCATGACCGGCCGAGCT
GTGAGCTGGTACTGGAAGGTGATGTGGGCTGGCGTAAGCCCACTGCTGATTGTCAGCCTC
TTTGTCTTCTACCTGAGCGACTACATCCTCACGGGGACCCTGAAGTATCAAGCCTGGGAC
GCCTCCCAGGGCCAGCTCGTGACCAAAGATTACCCGGCCTATGCACTGGCTGTCATCGGG
CTGCTTGTGGCCTCCTCCACCATGTGCATCCCCCTGGCGGCCCTGGGGACTTTTGTTCAG
CGTCGCCTCAAGAGGGGAGACGCAGACCCCGTGGCC

ORF Protein

Cloned ORF protein sequence for pFN21AE6468 Download

>KIEE6468  592 aa
MEKARPLWANSLQFVFACISYAVGLGNVWRFPYLCQMYGGGSFLVPYIIMLIVEGMPLLY
LELAVGQRMRQGSIGAWRTISPYLSGVGVASVVVSFFLSMYYNVINAWAFWYLFHSFQDP
LPWSVCPLNGNHTGYDEECEKASSTQYFWYRKTLNISPSLQENGGVQWEPALCLLLAWLV
VYLCILRGTESTGKVVYFTASLPYCVLIIYLIRGLTLHGATNGLMYMFTPKIEQLANPKA
WINAATQIFFSLGLGFGSLIAFASYNEPSNNCQKHAIIVSLINSFTSIFASIVTFSIYGF
KATFNYENCLKKVSLLLTNTFDLEDGFLTASNLEQVKGYLASAYPSKYSEMFPQIKNCSL
ESELDTAVQGTGLAFIVYTEAIKNMEVSQLWSVLYFFMLLMLGIGSMLGNTAAILTPLTD
SKIISSHLPKEAISGLVCLVNCAIGMVFTMEAGNYWFDIFNDYAATLSLLLIVLVETIAV
CYVYGLRRFESDLKAMTGRAVSWYWKVMWAGVSPLLIVSLFVFYLSDYILTGTLKYQAWD
ASQGQLVTKDYPAYALAVIGLLVASSTMCIPLAALGTFVQRRLKRGDADPVA

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE6468 Download

>pFN21AE6468  6481 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGAGAAAGCGCGGCCGCTGTGGGCCAACTCGCT
ACAGTTCGTGTTCGCCTGCATCTCGTACGCCGTGGGCCTGGGCAACGTGTGGCGATTCCC
GTACCTGTGCCAGATGTACGGCGGAGGTAGTTTCCTGGTCCCCTACATCATCATGCTTAT
CGTGGAGGGAATGCCGCTCTTGTACCTGGAACTGGCTGTGGGGCAGCGCATGCGGCAGGG
CAGCATCGGCGCCTGGAGGACCATCAGCCCGTACCTCAGTGGTGTCGGGGTCGCCAGCGT
GGTGGTCTCTTTCTTCCTCTCCATGTACTACAACGTCATCAACGCCTGGGCCTTCTGGTA
CCTCTTCCACTCCTTCCAGGATCCCCTGCCGTGGTCTGTCTGCCCACTGAATGGTAACCA
CACGGGCTACGATGAGGAGTGCGAGAAGGCGTCCTCCACACAGTACTTCTGGTACAGGAA
AACCCTCAATATCTCGCCGTCCCTCCAGGAGAACGGGGGTGTGCAGTGGGAGCCGGCGCT
GTGCCTCCTCCTGGCCTGGCTGGTGGTGTACCTGTGCATCCTGCGTGGCACCGAGTCCAC
TGGCAAGGTGGTGTATTTCACGGCGTCACTGCCCTATTGCGTGCTCATCATCTACCTCAT
CAGGGGCCTCACGCTCCACGGAGCCACCAATGGCCTCATGTACATGTTCACTCCCAAGAT
AGAGCAGCTGGCCAACCCCAAGGCCTGGATCAATGCAGCCACCCAGATCTTCTTCTCACT
TGGCCTGGGCTTCGGCAGCCTGATCGCCTTCGCCAGCTACAATGAGCCATCCAACAACTG
CCAGAAGCACGCCATCATCGTGTCCCTCATCAACAGCTTCACCTCCATATTTGCCAGCAT
TGTCACCTTCTCCATCTATGGCTTCAAGGCCACCTTCAATTATGAAAACTGCTTGAAGAA
GGTGAGTCTGCTGCTGACCAACACTTTTGACCTTGAAGATGGCTTTTTGACAGCCAGCAA
CCTGGAGCAGGTGAAGGGCTACCTCGCATCTGCCTACCCAAGCAAATACAGCGAGATGTT
CCCGCAAATCAAAAACTGCAGCTTGGAATCGGAGCTAGACACGGCCGTCCAGGGCACTGG
CCTGGCATTCATCGTCTATACAGAGGCCATTAAAAACATGGAGGTGTCCCAGCTGTGGTC
GGTGCTCTACTTCTTCATGCTGCTGATGCTGGGCATTGGGAGCATGCTGGGGAACACAGC
GGCCATCCTCACCCCTCTGACAGACAGCAAGATCATCTCCAGCCACCTGCCCAAGGAGGC
CATCTCAGGTCTGGTGTGCCTTGTCAACTGTGCCATTGGCATGGTGTTCACGATGGAGGC
TGGGAACTACTGGTTTGACATATTCAACGACTACGCGGCCACACTGTCCCTGCTGCTCAT
CGTGCTGGTGGAGACGATTGCCGTGTGCTACGTGTACGGGCTGAGGAGATTTGAAAGTGA
CCTTAAGGCCATGACCGGCCGAGCTGTGAGCTGGTACTGGAAGGTGATGTGGGCTGGCGT
AAGCCCACTGCTGATTGTCAGCCTCTTTGTCTTCTACCTGAGCGACTACATCCTCACGGG
GACCCTGAAGTATCAAGCCTGGGACGCCTCCCAGGGCCAGCTCGTGACCAAAGATTACCC
GGCCTATGCACTGGCTGTCATCGGGCTGCTTGTGGCCTCCTCCACCATGTGCATCCCCCT
GGCGGCCCTGGGGACTTTTGTTCAGCGTCGCCTCAAGAGGGGAGACGCAGACCCCGTGGC
CGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGC
AAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTG
AGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATT
GATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATT
TGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAAC
AATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAG
TAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTT
TCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGA
CTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGC
GGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAA
TAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTC
CGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAA
ACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAA
CTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATG
ATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAA
GAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTC
ACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACC
ATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTA
ACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAG
CTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACA
ACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATA
GACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGC
TGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCA
CTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCA
ACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGG
TAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAA
TACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGC
AAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCC
CTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTAT
AAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGC
CGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCT
CACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACG
AACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACC
CGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGA
GGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAA
GGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTA
GCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGC
AGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTG
ACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGA
TCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCG
CTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGC
CTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGC
AGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGC
GTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTA
GCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATC
T