(Linear) (Six-base) MAPSORT of: KIEE6468.seq Check: 2634 from: 1 to: 1776 KIEE6468.orf With 182 enzymes: SgfI * March 16, 2015 11:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 993 1289 1776 Size: 993 296 487 Fragments arranged by size: 993 487 296 Acc65I G'GTAC_C Cuts at: 0 332 1776 Size: 332 1444 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1621 1776 Size: 1621 155 AflII C'TTAA_G Cuts at: 0 1477 1776 Size: 1477 299 AflIII A'CryG_T Cuts at: 0 80 677 1445 1776 Size: 80 597 768 331 Fragments arranged by size: 768 597 331 80 BamHI G'GATC_C Cuts at: 0 354 1776 Size: 354 1422 BanI G'GyrC_C Cuts at: 0 223 332 562 1776 Size: 223 109 230 1214 Fragments arranged by size: 1214 230 223 109 BbeI G_GCGC'C Cuts at: 0 227 1776 Size: 227 1549 BbsI GAAGACnn'nnnn_ Cuts at: 0 1557 1776 Size: 1557 219 BbvCI CC'TCA_GC Cuts at: 0 1456 1776 Size: 1456 320 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 759 793 1776 Size: 759 34 983 Fragments arranged by size: 983 759 34 BglII A'GATC_T Cuts at: 0 740 1776 Size: 740 1036 Bme1580I G_kGCm'C Cuts at: 0 1111 1776 Size: 1111 665 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1314 1776 Size: 1314 462 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 249 463 1018 1257 1776 Size: 249 214 555 239 519 Fragments arranged by size: 555 519 249 239 214 Bpu10I CC'TnA_GC Cuts at: 0 1456 1573 1776 Size: 1456 117 203 Fragments arranged by size: 1456 203 117 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1732 1776 Size: 1732 44 BsaI GGTCTCn'nnnn_ Cuts at: 0 285 1776 Size: 285 1491 BsaAI yAC'GTr Cuts at: 0 1446 1776 Size: 1446 330 BsaHI Gr'CG_yC Cuts at: 0 224 424 598 1619 1776 Size: 224 200 174 1021 157 Fragments arranged by size: 1021 224 200 174 157 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 424 511 1473 1683 1776 Size: 424 87 962 210 93 Fragments arranged by size: 962 424 210 93 87 BseYI C'CCAG_C Cuts at: 0 1163 1203 1221 1354 1776 Size: 1163 40 18 133 422 Fragments arranged by size: 1163 422 133 40 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 517 1776 Size: 517 1259 BsiEI CG_ry'CG Cuts at: 0 16 1100 1494 1776 Size: 16 1084 394 282 Fragments arranged by size: 1084 394 282 16 BsiHKAI G_wGCw'C Cuts at: 0 620 1181 1776 Size: 620 561 595 Fragments arranged by size: 620 595 561 BsiWI C'GTAC_G Cuts at: 0 59 1776 Size: 59 1717 BsmI GAATG_Cn' Cuts at: 0 170 1120 1776 Size: 170 950 656 Fragments arranged by size: 950 656 170 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1420 1751 1776 Size: 1420 331 25 Fragments arranged by size: 1420 331 25 Bsp1286I G_dGCh'C Cuts at: 0 620 1111 1181 1776 Size: 620 491 70 595 Fragments arranged by size: 620 595 491 70 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 993 1289 1776 Size: 993 296 487 Fragments arranged by size: 993 487 296 BsrBI CCG'CTC Cuts at: 0 171 1776 Size: 171 1605 BsrFI r'CCGG_y Cuts at: 0 507 1489 1776 Size: 507 982 287 Fragments arranged by size: 982 507 287 BsrGI T'GTAC_A Cuts at: 0 674 1776 Size: 674 1102 BssSI C'ACGA_G Cuts at: 0 1636 1776 Size: 1636 140 BstAPI GCAn_nnn'nTGC Cuts at: 0 204 1776 Size: 204 1572 BstEII G'GTnAC_C Cuts at: 0 388 1776 Size: 388 1388 BstXI CCAn_nnnn'nTGG Cuts at: 0 276 996 1111 1335 1776 Size: 276 720 115 224 441 Fragments arranged by size: 720 441 276 224 115 BstYI r'GATC_y Cuts at: 0 354 740 1776 Size: 354 386 1036 Fragments arranged by size: 1036 386 354 BtgI C'CryG_G Cuts at: 0 65 364 651 1769 1776 Size: 65 299 287 1118 7 Fragments arranged by size: 1118 299 287 65 7 BtsI GCAGTG_nn' Cuts at: 0 505 601 1540 1776 Size: 505 96 939 236 Fragments arranged by size: 939 505 236 96 DraIII CAC_nnn'GTG Cuts at: 0 1703 1776 Size: 1703 73 DrdI GACnn_nn'nnGTC Cuts at: 0 1099 1776 Size: 1099 677 EaeI y'GGCC_r Cuts at: 0 13 704 1097 1235 1391 1491 1776 Size: 13 691 393 138 156 100 285 Fragments arranged by size: 691 393 285 156 138 100 13 EagI C'GGCC_G Cuts at: 0 13 1097 1491 1776 Size: 13 1084 394 285 Fragments arranged by size: 1084 394 285 13 EarI CTCTTCn'nnn_ Cuts at: 0 343 1776 Size: 343 1433 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 131 1776 Size: 131 1645 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 249 463 1018 1257 1621 1776 Size: 249 214 555 239 364 155 Fragments arranged by size: 555 364 249 239 214 155 EcoNI CCTnn'n_nnAGG Cuts at: 0 1000 1285 1776 Size: 1000 285 491 Fragments arranged by size: 1000 491 285 EcoO109I rG'GnC_Cy Cuts at: 0 639 1595 1776 Size: 639 956 181 Fragments arranged by size: 956 639 181 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 924 1776 Size: 924 852 HaeII r_GCGC'y Cuts at: 0 227 514 1776 Size: 227 287 1262 Fragments arranged by size: 1262 287 227 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 409 441 1776 Size: 409 32 1335 Fragments arranged by size: 1335 409 32 HincII GTy'rAC Cuts at: 0 1320 1776 Size: 1320 456 KasI G'GCGC_C Cuts at: 0 223 1776 Size: 223 1553 KpnI G_GTAC'C Cuts at: 0 336 1776 Size: 336 1440 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 830 1776 Size: 830 946 MscI TGG'CCA Cuts at: 0 706 1776 Size: 706 1070 MslI CAynn'nnrTG Cuts at: 0 145 676 832 1156 1333 1776 Size: 145 531 156 324 177 443 Fragments arranged by size: 531 443 324 177 156 145 MspA1I CmG'CkG Cuts at: 0 18 702 1167 1234 1776 Size: 18 684 465 67 542 Fragments arranged by size: 684 542 465 67 18 NaeI GCC'GGC Cuts at: 0 509 1776 Size: 509 1267 NarI GG'CG_CC Cuts at: 0 224 1776 Size: 224 1552 NgoMIV G'CCGG_C Cuts at: 0 507 1776 Size: 507 1269 NotI GC'GGCC_GC Cuts at: 0 13 1776 Size: 13 1763 NspI r_CATG'y Cuts at: 0 207 681 1221 1776 Size: 207 474 540 555 Fragments arranged by size: 555 540 474 207 PasI CC'CwG_GG Cuts at: 0 1626 1722 1776 Size: 1626 96 54 Fragments arranged by size: 1626 96 54 PciI A'CATG_T Cuts at: 0 677 1776 Size: 677 1099 PflMI CCAn_nnn'nTGG Cuts at: 0 385 719 1776 Size: 385 334 1057 Fragments arranged by size: 1057 385 334 PfoI T'CCnGG_A Cuts at: 0 350 479 1776 Size: 350 129 1297 Fragments arranged by size: 1297 350 129 PpuMI rG'GwC_Cy Cuts at: 0 1595 1776 Size: 1595 181 PstI C_TGCA'G Cuts at: 0 1075 1776 Size: 1075 701 PvuII CAG'CTG Cuts at: 0 702 1167 1776 Size: 702 465 609 Fragments arranged by size: 702 609 465 SanDI GG'GwC_CC Cuts at: 0 1595 1776 Size: 1595 181 ScaI AGT'ACT Cuts at: 0 439 1776 Size: 439 1337 SfcI C'TryA_G Cuts at: 0 34 1071 1776 Size: 34 1037 705 Fragments arranged by size: 1037 705 34 SfoI GGC'GCC Cuts at: 0 225 1776 Size: 225 1551 SmlI C'TyrA_G Cuts at: 0 1477 1747 1776 Size: 1477 270 29 Fragments arranged by size: 1477 270 29 SphI G_CATG'C Cuts at: 0 207 1221 1776 Size: 207 1014 555 Fragments arranged by size: 1014 555 207 StuI AGG'CCT Cuts at: 0 718 1776 Size: 718 1058 StyI C'CwwG_G Cuts at: 0 713 1286 1776 Size: 713 573 490 Fragments arranged by size: 713 573 490 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 751 1776 Size: 751 1025 TatI w'GTAC_w Cuts at: 0 299 437 674 1776 Size: 299 138 237 1102 Fragments arranged by size: 1102 299 237 138 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 579 1149 1302 1776 Size: 579 570 153 474 Fragments arranged by size: 579 570 474 153 Enzymes that do cut: AarI Acc65I AcuI AflII AflIII BamHI BanI BbeI BbsI BbvCI BcgI BglII Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI BtgI BtsI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NotI NspI PasI PciI PflMI PfoI PpuMI PstI PvuII SanDI ScaI SfcI SfoI SmlI SphI StuI StyI TaqII TatI XcmI Enzymes that do not cut: AatII AccI AclI AfeI AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BanII BciVI BclI BfrBI BglI BlpI BmgBI BmrI BmtI BsaBI BsaWI BsaXI BspEI BspHI BsrDI BssHII BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI EcoICRI EcoRI EcoRV FseI FspI FspAI HindIII HpaI MfeI MluI NcoI NdeI NheI NruI NsiI PacI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TstI Tth111I XbaI XhoI XmaI XmnI ZraI