################################## # NO CUT ENZYMES ( 107 enzymes) # ################################## AatII AccI AccIII AclI AcsI AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AvaI AviII AvrII BanII BbrPI BciVI BclI BglI BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BsoBI BspDI BspEI BspHI BsrDI BssHII Bst1107I BstBI BstZ17I Bsu36I CelII ClaI CpoI DraI Eam1105I Eco47III Eco81I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KspI MamI MfeI MluI MroI MunI NcoI NdeI NheI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI TaqI Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### Acc65I 332 GGTACC G^GTACC AflII 1477 CTTAAG C^TTAAG Asp718I 332 GGTACC G^GTACC BalI 704 TGGCCA TGG^CCA BamHI 354 GGATCC G^GATCC BbeI 223 GGCGCC GGCGC^C BbsI 1564 GTCTTC GAAGAC(2/6) BbvCI 1455 GCTGAGG CCTCAGC(-5/-2) BcgI 770 GCAGCCTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 1653 CCCGG CC^[GC]GG BfaI 1090 CTAG C^TAG BfrI 1477 CTTAAG C^TTAAG BglII 740 AGATCT A^GATCT BpuAI 1564 GTCTTC GAAGAC(2/6) BsaAI 1444 TACGTG [CT]AC^GT[GA] BsaI 279 GGTCTC GGTCTC(1/5) BsgI 496 GTGCAG GTGCAG(16/14) BsiWI 59 CGTACG C^GTACG Bsp1407I 674 TGTACA T^GTACA BspLU11I 677 ACATGT A^CATGT BsrBI 169 CCGCTC CCGCTC(-3/-3) BsrGI 674 TGTACA T^GTACA BssSI 1636 CTCGTG CACGAG(-5/-1) BstAPI 198 GCAGCGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 388 GGTAACC G^GT[ACGT]ACC BstPI 388 GGTAACC G^GT[ACGT]ACC DraIII 1698 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1093 GACACGGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 337 CTCTTC CTCTTC(1/4) EcoO65I 388 GGTAACC G^GT[ACGT]ACC FauI 1056 CCCGC CCCGC(4/6) HincII 1318 GTCAAC GT[CT]^[GA]AC HindII 1318 GTCAAC GT[CT]^[GA]AC KasI 223 GGCGCC G^GCGCC KpnI 332 GGTACC GGTAC^C Ksp632I 337 CTCTTC CTCTTC(1/4) MaeI 1090 CTAG C^TAG MluNI 704 TGGCCA TGG^CCA MscI 704 TGGCCA TGG^CCA NaeI 507 GCCGGC GCC^GGC NarI 223 GGCGCC GG^CGCC NciI 1653 CCCGG CC^[GC]GG NgoMIV 507 GCCGGC G^CCGGC NotI 12 GCGGCCGC GC^GGCCGC PpuMI 1594 GGGACCC [GA]G^G[AT]CC[CT] PstI 1071 CTGCAG CTGCA^G ScaI 437 AGTACT AGT^ACT SfoI 223 GGCGCC GGC^GCC SspBI 674 TGTACA T^GTACA StuI 716 AGGCCT AGG^CCT Tsp509I 913 AATT ^AATT ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccII 11 CGCG CG^CG 1389 CGCG AspHI 616 GTGCTC G[AT]GC[AT]^C 1177 GTGCTC BsiHKAI 616 GTGCTC G[AT]GC[AT]^C 1177 GTGCTC BsmBI 1426 GAGACG CGTCTC(1/5) 1757 GAGACG BsmI 164 GAATGC GAATGC(1/-1) 1120 GCATTC BspMI 1280 ACCTGC ACCTGC(4/8) 1002 GCAGGT BsrFI 507 GCCGGC [GA]^CCGG[CT] 1489 ACCGGC BstUI 11 CGCG CG^CG 1389 CGCG BstYI 354 GGATCC [GA]^GATC[CT] 740 AGATCT Cfr10I 507 GCCGGC [GA]^CCGG[CT] 1489 ACCGGC DraII 638 GGGGCCT [GA]G^G[ACGT]CC[CT] 1594 GGGACCC EcoNI 996 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1281 CCTGCCCAAGG EcoO109I 638 GGGGCCT [GA]G^G[ACGT]CC[CT] 1594 GGGACCC EcoT14I 713 CCAAGG C^C[AT][AT]GG 1286 CCAAGG HaeII 223 GGCGCC [GA]GCGC^[CT] 510 GGCGCT MflI 354 GGATCC [GA]^GATC[CT] 740 AGATCT MseI 1145 TTAA T^TAA 1478 TTAA MvnI 11 CGCG CG^CG 1389 CGCG PflMI 379 CCACTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 713 CCAAGGCCTGG PleI 568 GAGTC GAGTC(4/5) 939 GAGTC PvuII 700 CAGCTG CAG^CTG 1165 CAGCTG SfcI 34 CTACAG C^T[GA][CT]AG 1071 CTGCAG SmlI 1477 CTTAAG C^T[CT][GA]AG 1747 CTCAAG SphI 203 GCATGC GCATG^C 1217 GCATGC StyI 713 CCAAGG C^C[AT][AT]GG 1286 CCAAGG TfiI 89 GATTC G^A[AT]TC 1081 GAATC Tru9I 1145 TTAA T^TAA 1478 TTAA Van91I 379 CCACTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 713 CCAAGGCCTGG XhoII 354 GGATCC [GA]^GATC[CT] 740 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AciI AcyI AfaI AflIII AluI AlwI AvaII BanI BbvI BmyI BpmI BsaHI BsaJI BseRI BsiEI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EagI EclXI Eco52I EcoRII Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI RsaI Sau3AI Sau96I ScrFI SfaNI TseI Tsp45I TspRI XcmI