PCOLCE ORF clone pFN21AE6443

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE6443

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Clone info.

Species

human

Clone Name

pFN21AE6443 Sequence (with vector) Restriction Map

Product ID

FHC09726

ORF Sequence

DNA sequence (1347 bp)
Protein sequence (449 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE6443

Symbol

PCOLCE

Alias : PCPE, PCPE-1, PCPE1

Description

procollagen C-endopeptidase enhancer

Original Clone ID

oc96443

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1347 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 449 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS5700.1 2e-137 100.0 PCOLCE CCDS3127.1 4e-42 44.5 PCOLCE2 CCDS31179.1 3.4e-19 33.5 NRP1 CCDS31180.1 3.6e-19 33.5 NRP1 CCDS81448.1 4.7e-19 33.5 NRP1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000859 37 146 PF00431 CUB IPR000859 159 270 PF00431 CUB IPR018933 331 433 PF01759 Netrin module HMMSmart IPR000859 37 149 SM00042 CUB IPR000859 159 273 SM00042 CUB IPR018933 329 435 SM00643 Netrin module ProfileScan IPR000859 37 149 PS01180 CUB IPR000859 159 273 PS01180 CUB IPR001134 318 437 PS50189 Netrin domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_002584 449 600270 100.0 100.0 NP_037495 415 607064 44.5 96.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE6443 Download

>KIEE6443  1347 bp
ATGCTGCCTGCAGCCACAGCCTCCCTCCTGGGGCCCCTCCTCACTGCCTGCGCCCTGCTG
CCTTTTGCCCAGGGCCAGACCCCCAACTACACCAGACCCGTGTTCCTGTGCGGAGGGGAT
GTGAAGGGGGAATCAGGTTACGTGGCAAGTGAGGGGTTCCCCAACCTCTACCCCCCTAAT
AAGGAGTGCATCTGGACCATAACGGTCCCCGAGGGCCAGACTGTGTCCCTCTCATTCCGA
GTCTTCGACCTGGAGCTGCACCCCGCCTGCCGCTACGATGCTCTGGAGGTCTTCGCTGGG
TCTGGGACTTCCGGCCAGCGGCTCGGACGCTTTTGTGGGACCTTCCGGCCTGCGCCCCTA
GTCGCCCCCGGCAACCAGGTGACCCTGAGGATGACGACGGATGAGGGCACAGGAGGACGA
GGCTTCCTGCTCTGGTACAGCGGGCGGGCCACCTCGGGCACTGAGCACCAATTTTGCGGG
GGGCGGCTGGAGAAGGCCCAGGGAACCCTGACCACGCCCAACTGGCCCGAGTCCGATTAC
CCCCCGGGCATCAGCTGTTCCTGGCACATCATCGCGCCCCCGGACCAGGTCATCGCGCTG
ACCTTCGAGAAGTTTGACCTGGAGCCGGACACCTACTGCCGCTATGACTCGGTCAGCGTG
TTCAACGGAGCCGTGAGCGACGACTCCCGGAGGCTGGGGAAGTTCTGCGGCGACGCAGTC
CCGGGCTCCATCTCCTCCGAAGGGAATGAACTCCTCGTCCAGTTCGTCTCAGATCTCAGT
GTCACCGCTGATGGCTTCTCAGCCTCCTACAAGACCCTGCCGCGGGGCACTGCCAAAGAA
GGGCAAGGGCCCGGCCCCAAACGGGGAACTGAGCCTAAAGTCAAGCTGCCCCCCAAGTCC
CAACCTCCGGAGAAAACAGAGGAATCTCCTTCAGCCCCTGATGCACCCACCTGCCCAAAG
CAGTGCCGCCGGACAGGCACCTTGCAGAGCAACTTCTGTGCCAGCAGCCTTGTGGTGACT
GCGACAGTGAAGTCCATGGTTCGGGAGCCAGGGGAGGGCCTTGCCGTGACTGTCAGTCTT
ATTGGTGCTTATAAAACTGGAGGACTGGACCTGCCTTCTCCACCCACTGGTGCCTCCCTG
AAGTTTTACGTGCCTTGCAAGCAGTGCCCCCCCATGAAGAAAGGAGTCAGTTATCTGCTG
ATGGGCCAGGTAGAAGAGAACAGAGGCCCCGTCCTTCCTCCAGAGAGCTTTGTGGTTCTC
CACCGGCCCAACCAGGACCAGATCCTCACCAACCTAAGCAAGAGGAAGTGCCCCTCTCAA
CCTGTGCGGGCTGCTGCGTCCCAGGAC

ORF Protein

Cloned ORF protein sequence for pFN21AE6443 Download

>KIEE6443  449 aa
MLPAATASLLGPLLTACALLPFAQGQTPNYTRPVFLCGGDVKGESGYVASEGFPNLYPPN
KECIWTITVPEGQTVSLSFRVFDLELHPACRYDALEVFAGSGTSGQRLGRFCGTFRPAPL
VAPGNQVTLRMTTDEGTGGRGFLLWYSGRATSGTEHQFCGGRLEKAQGTLTTPNWPESDY
PPGISCSWHIIAPPDQVIALTFEKFDLEPDTYCRYDSVSVFNGAVSDDSRRLGKFCGDAV
PGSISSEGNELLVQFVSDLSVTADGFSASYKTLPRGTAKEGQGPGPKRGTEPKVKLPPKS
QPPEKTEESPSAPDAPTCPKQCRRTGTLQSNFCASSLVVTATVKSMVREPGEGLAVTVSL
IGAYKTGGLDLPSPPTGASLKFYVPCKQCPPMKKGVSYLLMGQVEENRGPVLPPESFVVL
HRPNQDQILTNLSKRKCPSQPVRAAASQD

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE6443 Download

>pFN21AE6443  6052 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCTGCCTGCAGCCACAGCCTCCCTCCTGGGGCC
CCTCCTCACTGCCTGCGCCCTGCTGCCTTTTGCCCAGGGCCAGACCCCCAACTACACCAG
ACCCGTGTTCCTGTGCGGAGGGGATGTGAAGGGGGAATCAGGTTACGTGGCAAGTGAGGG
GTTCCCCAACCTCTACCCCCCTAATAAGGAGTGCATCTGGACCATAACGGTCCCCGAGGG
CCAGACTGTGTCCCTCTCATTCCGAGTCTTCGACCTGGAGCTGCACCCCGCCTGCCGCTA
CGATGCTCTGGAGGTCTTCGCTGGGTCTGGGACTTCCGGCCAGCGGCTCGGACGCTTTTG
TGGGACCTTCCGGCCTGCGCCCCTAGTCGCCCCCGGCAACCAGGTGACCCTGAGGATGAC
GACGGATGAGGGCACAGGAGGACGAGGCTTCCTGCTCTGGTACAGCGGGCGGGCCACCTC
GGGCACTGAGCACCAATTTTGCGGGGGGCGGCTGGAGAAGGCCCAGGGAACCCTGACCAC
GCCCAACTGGCCCGAGTCCGATTACCCCCCGGGCATCAGCTGTTCCTGGCACATCATCGC
GCCCCCGGACCAGGTCATCGCGCTGACCTTCGAGAAGTTTGACCTGGAGCCGGACACCTA
CTGCCGCTATGACTCGGTCAGCGTGTTCAACGGAGCCGTGAGCGACGACTCCCGGAGGCT
GGGGAAGTTCTGCGGCGACGCAGTCCCGGGCTCCATCTCCTCCGAAGGGAATGAACTCCT
CGTCCAGTTCGTCTCAGATCTCAGTGTCACCGCTGATGGCTTCTCAGCCTCCTACAAGAC
CCTGCCGCGGGGCACTGCCAAAGAAGGGCAAGGGCCCGGCCCCAAACGGGGAACTGAGCC
TAAAGTCAAGCTGCCCCCCAAGTCCCAACCTCCGGAGAAAACAGAGGAATCTCCTTCAGC
CCCTGATGCACCCACCTGCCCAAAGCAGTGCCGCCGGACAGGCACCTTGCAGAGCAACTT
CTGTGCCAGCAGCCTTGTGGTGACTGCGACAGTGAAGTCCATGGTTCGGGAGCCAGGGGA
GGGCCTTGCCGTGACTGTCAGTCTTATTGGTGCTTATAAAACTGGAGGACTGGACCTGCC
TTCTCCACCCACTGGTGCCTCCCTGAAGTTTTACGTGCCTTGCAAGCAGTGCCCCCCCAT
GAAGAAAGGAGTCAGTTATCTGCTGATGGGCCAGGTAGAAGAGAACAGAGGCCCCGTCCT
TCCTCCAGAGAGCTTTGTGGTTCTCCACCGGCCCAACCAGGACCAGATCCTCACCAACCT
AAGCAAGAGGAAGTGCCCCTCTCAACCTGTGCGGGCTGCTGCGTCCCAGGACGTTTAAAC
GAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATC
CGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAAC
TAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTT
GGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCT
ATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATT
CATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTC
TACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTC
CAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTC
TACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCT
ATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGA
TAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCC
CTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTG
AAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTC
AACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACT
TTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTC
GGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAG
CATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGAT
AACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTT
TTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAA
GCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGC
AAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATG
GAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATT
GCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA
GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAT
GAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAA
ATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTAT
CCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCA
GGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGC
ATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACC
AGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCG
GATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTA
GGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCG
TTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGAC
ACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAG
GCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTAT
TTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGAT
CCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGC
GCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGT
GGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCT
AGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGG
TGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCT
CTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCG
GGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCG
GTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAG
ATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT