(Circular) (Six-base) MAPSORT of: pFN21AE6443.seq Check: 1189 from: 1 to: 6052 pFN21AE6443 6052 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2963 2963 Size: 6052 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3862 278 Size: 53 83 186 936 2326 2468 Fragments arranged by size: 2468 2326 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3395 1937 Size: 1458 4594 Acc65I G'GTAC_C Cuts at: 3373 3373 Size: 6052 AclI AA'CG_TT Cuts at: 1563 4179 4552 1563 Size: 2616 373 3063 Fragments arranged by size: 3063 2616 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2919 3164 4115 5499 5767 1632 Size: 1287 245 951 1384 268 1917 Fragments arranged by size: 1917 1384 1287 951 268 245 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5855 AflIII A'CryG_T Cuts at: 1203 4951 5928 1203 Size: 3748 977 1327 Fragments arranged by size: 3748 1327 977 AgeI A'CCGG_T Cuts at: 4883 4883 Size: 6052 AhdI GACnn_n'nnGTC Cuts at: 3078 4781 3078 Size: 1703 4349 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6052 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2971 1184 Size: 1787 4265 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2745 2777 2745 Size: 32 6020 AlwNI CAG_nnn'CTG Cuts at: 2942 3795 5367 2942 Size: 853 1572 3627 Fragments arranged by size: 3627 1572 853 ApaI G_GGCC'C Cuts at: 2040 2856 2040 Size: 816 5236 ApaLI G'TGCA_C Cuts at: 4109 5265 4109 Size: 1156 4896 ApoI r'AATT_y Cuts at: 1493 3361 3587 3739 5848 1493 Size: 1868 226 152 2109 1697 Fragments arranged by size: 2109 1868 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4606 160 Size: 872 3574 1606 Fragments arranged by size: 3574 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6052 AvaI C'yCGr_G Cuts at: 1870 1943 2213 2458 2531 2548 2725 3377 1870 Size: 73 270 245 73 17 177 652 4545 Fragments arranged by size: 4545 652 270 245 177 73 73 17 BamHI G'GATC_C Cuts at: 3382 3749 3382 Size: 367 5685 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2981 3134 3373 4828 618 Size: 325 217 627 1194 153 239 1455 1842 Fragments arranged by size: 1842 1455 1194 627 325 239 217 153 BanII G_rGCy'C Cuts at: 1391 2040 2732 2856 3371 1391 Size: 649 692 124 515 4072 Fragments arranged by size: 4072 692 649 515 124 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2239 2287 928 Size: 971 340 48 4693 Fragments arranged by size: 4693 971 340 48 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4243 4277 1903 Size: 34 50 34 2222 34 3678 Fragments arranged by size: 3678 2222 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3944 5154 3944 Size: 1210 4842 BclI T'GATC_A Cuts at: 1548 1548 Size: 6052 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2527 2979 4663 Size: 107 122 71 631 605 855 452 1684 Cuts at: 4663 136 Size: 1525 Fragments arranged by size: 1684 1525 855 631 605 452 122 107 71 BglII A'GATC_T Cuts at: 2776 6047 2776 Size: 3271 2781 BlpI GC'TnA_GC Cuts at: 3469 3469 Size: 6052 Bme1580I G_kGCm'C Cuts at: 1790 2040 2414 2465 2834 2856 3173 3317 4113 Size: 250 374 51 369 22 317 144 796 Cuts at: 4113 5269 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 796 374 369 317 250 144 51 22 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6052 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3783 4741 442 Size: 416 53 83 399 24 2366 958 1753 Fragments arranged by size: 2366 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6052 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2276 2309 2513 2645 3123 3228 4712 1239 Size: 1037 33 204 132 478 105 1484 2579 Fragments arranged by size: 2579 1484 1037 478 204 132 105 33 Bpu10I CC'TnA_GC Cuts at: 3299 3299 Size: 6052 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4177 5042 5340 4177 Size: 865 298 4889 Fragments arranged by size: 4889 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4715 882 Size: 617 3216 2219 Fragments arranged by size: 3216 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2146 3154 3843 493 Size: 711 942 1008 689 2702 Fragments arranged by size: 2702 1008 942 711 689 BsaBI GATnn'nnATC Cuts at: 6046 6046 Size: 6052 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3859 4241 4876 275 Size: 53 83 186 936 2326 382 635 1451 Fragments arranged by size: 2326 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2911 4485 4883 5157 5304 5715 2911 Size: 1574 398 274 147 411 3248 Fragments arranged by size: 3248 1574 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2109 2139 1178 Size: 30 165 30 706 30 5091 Fragments arranged by size: 5091 706 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2033 2728 2747 1186 Size: 847 695 19 4491 Fragments arranged by size: 4491 847 695 19 BseYI C'CCAG_C Cuts at: 2300 2698 5255 5751 2300 Size: 398 2557 496 2601 Fragments arranged by size: 2601 2557 496 398 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2246 1163 Size: 573 510 4969 Fragments arranged by size: 4969 573 510 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3502 4263 5291 1540 Size: 275 186 1501 761 1028 2301 Fragments arranged by size: 2301 1501 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2472 4113 4198 5269 2472 Size: 1641 85 1071 3255 Fragments arranged by size: 3255 1641 1071 85 BsmI GAATG_Cn' Cuts at: 3563 3656 3563 Size: 93 5959 BsmBI CGTCTCn'nnnn_ Cuts at: 2776 2776 Size: 6052 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 2414 2465 2472 2732 2834 2856 Size: 399 250 374 51 7 260 102 22 Cuts at: 2856 3173 3317 3371 4113 4198 5269 1391 Size: 317 144 54 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 374 317 260 250 144 102 85 54 51 22 7 BspEI T'CCGG_A Cuts at: 2911 5715 2911 Size: 2804 3248 BspHI T'CATG_A Cuts at: 3941 5671 3941 Size: 1730 4322 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2963 3123 3407 844 Size: 2119 160 284 3489 Fragments arranged by size: 3489 2119 284 160 BsrBI CCG'CTC Cuts at: 1394 1823 3939 1394 Size: 429 2116 3507 Fragments arranged by size: 3507 2116 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4547 4721 65 Size: 1183 3299 174 1396 Fragments arranged by size: 3299 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3267 4696 4883 1700 Size: 110 1457 1429 187 2869 Fragments arranged by size: 2869 1457 1429 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6052 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6052 BssSI C'ACGA_G Cuts at: 4112 5124 4112 Size: 1012 5040 BstAPI GCAn_nnn'nTGC Cuts at: 794 3168 794 Size: 2374 3678 BstBI TT'CG_AA Cuts at: 4856 4856 Size: 6052 BstEII G'GTnAC_C Cuts at: 1184 2383 1184 Size: 1199 4853 BstYI r'GATC_y Cuts at: 1972 2776 3285 3382 3749 4134 4151 5603 5689 Size: 804 509 97 367 385 17 1452 86 Cuts at: 5689 5701 6047 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 804 509 385 367 346 97 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2390 2390 Size: 6052 BtgI C'CryG_G Cuts at: 513 1065 2825 3039 513 Size: 552 1760 214 3526 Fragments arranged by size: 3526 1760 552 214 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2560 2581 487 Size: 683 56 1334 21 3958 Fragments arranged by size: 3958 1334 683 56 21 BtsI GCAGTG_nn' Cuts at: 2046 2832 2972 3173 3568 4362 4382 2046 Size: 786 140 201 395 794 20 3716 Fragments arranged by size: 3716 794 786 395 201 140 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6052 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6017 DraI TTT'AAA Cuts at: 3356 3356 Size: 6052 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3034 5059 809 Size: 507 1718 2025 1802 Fragments arranged by size: 2025 1802 1718 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2317 3499 4388 Size: 54 1425 50 275 18 487 1182 889 Cuts at: 4388 6017 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1182 889 487 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3499 1537 Size: 275 1687 4090 Fragments arranged by size: 4090 1687 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3213 3982 1353 Size: 1860 769 3423 Fragments arranged by size: 3423 1860 769 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4639 5013 5159 387 Size: 1064 138 3050 374 146 1280 Fragments arranged by size: 3050 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2276 2309 2513 2645 2919 3123 3164 Size: 393 644 33 204 132 274 204 41 Cuts at: 3164 3228 4115 4712 5499 5767 1239 Size: 64 887 597 787 268 1524 Fragments arranged by size: 1524 887 787 644 597 393 274 268 204 204 132 64 41 33 EcoO109I rG'GnC_Cy Cuts at: 2036 2037 2343 2852 3061 3230 2036 Size: 1 306 509 209 169 4858 Fragments arranged by size: 4858 509 306 209 169 1 EcoRI G'AATT_C Cuts at: 3361 3739 3361 Size: 378 5674 FspI TGC'GCA Cuts at: 4558 4558 Size: 6052 HaeII r_GCGC'y Cuts at: 1678 5199 1678 Size: 3521 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2786 2818 3064 3096 3219 Size: 32 135 32 1214 32 246 32 123 Cuts at: 3219 3251 4693 4725 4767 4799 1373 Size: 32 1442 32 42 32 2626 Fragments arranged by size: 2626 1442 1214 246 135 123 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3396 3642 669 Size: 988 281 1458 246 3079 Fragments arranged by size: 3079 1458 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6052 HpaI GTT'AAC Cuts at: 3642 3642 Size: 6052 KpnI G_GTAC'C Cuts at: 3377 3377 Size: 6052 MfeI C'AATT_G Cuts at: 3651 3651 Size: 6052 MluI A'CGCG_T Cuts at: 5928 5928 Size: 6052 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5166 5350 1305 Size: 3861 184 2007 Fragments arranged by size: 3861 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4230 Fragments arranged by size: 4230 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4010 4369 4528 518 Size: 648 2844 359 159 2042 Fragments arranged by size: 2844 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2323 2445 2559 2792 2827 3468 4145 5293 Size: 717 122 114 233 35 641 677 1148 Cuts at: 5293 5538 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 717 677 641 245 233 122 114 35 NcoI C'CATG_G Cuts at: 513 1065 3039 513 Size: 552 1974 3526 Fragments arranged by size: 3526 1974 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6052 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6052 NotI GC'GGCC_GC Cuts at: 3499 3499 Size: 6052 NspI r_CATG'y Cuts at: 1132 1229 3410 4955 1132 Size: 97 2181 1545 2229 Fragments arranged by size: 2229 2181 1545 97 PasI CC'CwG_GG Cuts at: 1348 2074 2503 1348 Size: 726 429 4897 Fragments arranged by size: 4897 726 429 PciI A'CATG_T Cuts at: 4951 4951 Size: 6052 PflMI CCAn_nnn'nTGG Cuts at: 1108 3131 1108 Size: 2023 4029 PfoI T'CCnGG_A Cuts at: 2690 2690 Size: 6052 PmeI GTTT'AAAC Cuts at: 3356 3356 Size: 6052 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4121 4153 5634 5666 4121 Size: 32 1481 32 4507 Fragments arranged by size: 4507 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2343 2343 Size: 6052 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6052 PsiI TTA'TAA Cuts at: 3096 3622 3096 Size: 526 5526 PspOMI G'GGCC_C Cuts at: 2036 2852 2036 Size: 816 5236 PstI C_TGCA'G Cuts at: 830 2017 3404 830 Size: 1187 1387 3478 Fragments arranged by size: 3478 1387 1187 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6052 PvuII CAG'CTG Cuts at: 2559 2559 Size: 6052 SacII CC_GC'GG Cuts at: 2828 2828 Size: 6052 SalI G'TCGA_C Cuts at: 1936 3394 1936 Size: 1458 4594 SbfI CC_TGCA'GG Cuts at: 3404 3404 Size: 6052 ScaI AGT'ACT Cuts at: 4300 6012 4300 Size: 1712 4340 SexAI A'CCwGG_T Cuts at: 2379 2589 2379 Size: 210 5842 SfcI C'TryA_G Cuts at: 826 1045 2013 3400 4535 5216 5407 826 Size: 219 968 1387 1135 681 191 1471 Fragments arranged by size: 1471 1387 1135 968 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6052 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6052 SmaI CCC'GGG Cuts at: 1872 2550 2727 3379 1872 Size: 678 177 652 4545 Fragments arranged by size: 4545 678 652 177 SmlI C'TyrA_G Cuts at: 820 1017 1943 4156 5057 5319 820 Size: 197 926 2213 901 262 1553 Fragments arranged by size: 2213 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3843 493 Size: 3350 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6052 SphI G_CATG'C Cuts at: 1132 3410 1132 Size: 2278 3774 SspI AAT'ATT Cuts at: 5 52 3976 5 Size: 47 3924 2081 Fragments arranged by size: 3924 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6052 StyI C'CwwG_G Cuts at: 513 1065 3039 3491 513 Size: 552 1974 452 3074 Fragments arranged by size: 3074 1974 552 452 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2644 2965 3142 4079 4096 4249 4434 Size: 1255 1064 321 177 937 17 153 185 Cuts at: 4434 4879 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1064 937 445 321 185 177 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4298 6010 96 Size: 274 80 33 51 1443 2321 1712 138 Fragments arranged by size: 2321 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2109 2141 6012 6044 2109 Size: 32 3871 32 2117 Fragments arranged by size: 3871 2117 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2227 2591 2654 2720 1316 Size: 911 364 63 66 4648 Fragments arranged by size: 4648 911 364 66 63 XbaI T'CTAG_A Cuts at: 3388 3388 Size: 6052 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3013 3013 Size: 6052 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6052 XmaI C'CCGG_G Cuts at: 1870 2548 2725 3377 1870 Size: 678 177 652 4545 Fragments arranged by size: 4545 678 652 177 XmnI GAAnn'nnTTC Cuts at: 4181 4181 Size: 6052 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3860 276 Size: 53 83 186 936 2326 2468 Fragments arranged by size: 2468 2326 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SanDI SapI SfiI SfoI SrfI SwaI