(Linear) (Six-base) MAPSORT of: KIEE6443.seq Check: 3604 from: 1 to: 1347 KIEE6443.orf With 182 enzymes: SgfI * March 16, 2015 11:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 958 1347 Size: 958 389 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 914 1159 1347 Size: 914 245 188 Fragments arranged by size: 914 245 188 AhdI GACnn_n'nnGTC Cuts at: 0 1073 1347 Size: 1073 274 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 966 1347 Size: 966 381 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 740 772 1347 Size: 740 32 575 Fragments arranged by size: 740 575 32 AlwNI CAG_nnn'CTG Cuts at: 0 937 1347 Size: 937 410 ApaI G_GGCC'C Cuts at: 0 35 851 1347 Size: 35 816 496 Fragments arranged by size: 816 496 35 AvaI C'yCGr_G Cuts at: 0 208 453 526 543 720 1347 Size: 208 245 73 17 177 627 Fragments arranged by size: 627 245 208 177 73 17 BanI G'GyrC_C Cuts at: 0 976 1129 1347 Size: 976 153 218 Fragments arranged by size: 976 218 153 BanII G_rGCy'C Cuts at: 0 35 727 851 1347 Size: 35 692 124 496 Fragments arranged by size: 692 496 124 35 BbsI GAAGACnn'nnnn_ Cuts at: 0 234 282 1347 Size: 234 48 1065 Fragments arranged by size: 1065 234 48 BglI GCCn_nnn'nGGC Cuts at: 0 522 974 1347 Size: 522 452 373 Fragments arranged by size: 522 452 373 BglII A'GATC_T Cuts at: 0 771 1347 Size: 771 576 Bme1580I G_kGCm'C Cuts at: 0 35 409 460 829 851 1168 1312 1347 Size: 35 374 51 369 22 317 144 35 Fragments arranged by size: 374 369 317 144 51 35 35 22 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 271 304 508 640 1118 1223 1347 Size: 271 33 204 132 478 105 124 Fragments arranged by size: 478 271 204 132 124 105 33 Bpu10I CC'TnA_GC Cuts at: 0 1294 1347 Size: 1294 53 BsaAI yAC'GTr Cuts at: 0 141 1149 1347 Size: 141 1008 198 Fragments arranged by size: 1008 198 141 BsaWI w'CCGG_w Cuts at: 0 906 1347 Size: 906 441 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 104 134 1347 Size: 104 30 1213 Fragments arranged by size: 1213 104 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 28 723 742 1347 Size: 28 695 19 605 Fragments arranged by size: 695 605 28 19 BseYI C'CCAG_C Cuts at: 0 295 693 1347 Size: 295 398 654 Fragments arranged by size: 654 398 295 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 241 1347 Size: 241 1106 BsiHKAI G_wGCw'C Cuts at: 0 467 1347 Size: 467 880 BsmBI CGTCTCn'nnnn_ Cuts at: 0 771 1347 Size: 771 576 Bsp1286I G_dGCh'C Cuts at: 0 35 409 460 467 727 829 851 1168 Size: 35 374 51 7 260 102 22 317 Cuts at: 1168 1312 1347 Size: 144 35 Fragments arranged by size: 374 317 260 144 102 51 35 35 22 7 BspEI T'CCGG_A Cuts at: 0 906 1347 Size: 906 441 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 958 1118 1347 Size: 958 160 229 Fragments arranged by size: 958 229 160 BsrFI r'CCGG_y Cuts at: 0 1262 1347 Size: 1262 85 BstAPI GCAn_nnn'nTGC Cuts at: 0 1163 1347 Size: 1163 184 BstEII G'GTnAC_C Cuts at: 0 378 1347 Size: 378 969 BstYI r'GATC_y Cuts at: 0 771 1280 1347 Size: 771 509 67 Fragments arranged by size: 771 509 67 Bsu36I CC'TnA_GG Cuts at: 0 385 1347 Size: 385 962 BtgI C'CryG_G Cuts at: 0 820 1034 1347 Size: 820 214 313 Fragments arranged by size: 820 313 214 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 555 576 1347 Size: 555 21 771 Fragments arranged by size: 771 555 21 BtsI GCAGTG_nn' Cuts at: 0 41 827 967 1168 1347 Size: 41 786 140 201 179 Fragments arranged by size: 786 201 179 140 41 DrdI GACnn_nn'nnGTC Cuts at: 0 1029 1347 Size: 1029 318 EaeI y'GGCC_r Cuts at: 0 312 1347 Size: 312 1035 EarI CTCTTCn'nnn_ Cuts at: 0 1208 1347 Size: 1208 139 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 271 304 508 640 914 1118 1159 1223 Size: 271 33 204 132 274 204 41 64 Cuts at: 1223 1347 Size: 124 Fragments arranged by size: 274 271 204 204 132 124 64 41 33 EcoO109I rG'GnC_Cy Cuts at: 0 31 32 338 847 1056 1225 1347 Size: 31 1 306 509 209 169 122 Fragments arranged by size: 509 306 209 169 122 31 1 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 781 813 1059 1091 1214 1246 1347 Size: 781 32 246 32 123 32 101 Fragments arranged by size: 781 246 123 101 32 32 32 MspA1I CmG'CkG Cuts at: 0 318 440 554 787 822 1347 Size: 318 122 114 233 35 525 Fragments arranged by size: 525 318 233 122 114 35 NcoI C'CATG_G Cuts at: 0 1034 1347 Size: 1034 313 PasI CC'CwG_GG Cuts at: 0 69 498 1347 Size: 69 429 849 Fragments arranged by size: 849 429 69 PflMI CCAn_nnn'nTGG Cuts at: 0 1126 1347 Size: 1126 221 PfoI T'CCnGG_A Cuts at: 0 685 1347 Size: 685 662 PpuMI rG'GwC_Cy Cuts at: 0 338 1347 Size: 338 1009 PsiI TTA'TAA Cuts at: 0 1091 1347 Size: 1091 256 PspOMI G'GGCC_C Cuts at: 0 31 847 1347 Size: 31 816 500 Fragments arranged by size: 816 500 31 PstI C_TGCA'G Cuts at: 0 12 1347 Size: 12 1335 PvuII CAG'CTG Cuts at: 0 554 1347 Size: 554 793 SacII CC_GC'GG Cuts at: 0 823 1347 Size: 823 524 SexAI A'CCwGG_T Cuts at: 0 374 584 1347 Size: 374 210 763 Fragments arranged by size: 763 374 210 SfcI C'TryA_G Cuts at: 0 8 1347 Size: 8 1339 SmaI CCC'GGG Cuts at: 0 545 722 1347 Size: 545 177 625 Fragments arranged by size: 625 545 177 StyI C'CwwG_G Cuts at: 0 1034 1347 Size: 1034 313 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 639 960 1137 1347 Size: 639 321 177 210 Fragments arranged by size: 639 321 210 177 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 104 136 1347 Size: 104 32 1211 Fragments arranged by size: 1211 104 32 Tth111I GACn'n_nGTC Cuts at: 0 222 586 649 715 1347 Size: 222 364 63 66 632 Fragments arranged by size: 632 364 222 66 63 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1008 1347 Size: 1008 339 XmaI C'CCGG_G Cuts at: 0 543 720 1347 Size: 543 177 627 Fragments arranged by size: 627 543 177 Enzymes that do cut: AarI AcuI AhdI AlfI AloI AlwNI ApaI AvaI BanI BanII BbsI BglI BglII Bme1580I BpmI Bpu10I BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrFI BstAPI BstEII BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI EarI Eco57MI EcoO109I Hin4I MspA1I NcoI PasI PflMI PfoI PpuMI PsiI PspOMI PstI PvuII SacII SexAI SfcI SmaI StyI TaqII TstI Tth111I XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AleI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BlpI BmgBI BmrI BmtI BplI BpuEI BsaI BsaBI BsaHI BsiEI BsiWI BsmI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstXI BstZ17I ClaI CspCI DraI DraIII EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PpiI PshAI PspXI PsrI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI XbaI XhoI XmnI ZraI