################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AcyI AflII AflIII AgeI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsiEI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstXI BstZ17I CelII ClaI CpoI DraI DraIII EagI EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI MamI MfeI MluI MluNI MscI MseI MslI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccIII 906 TCCGGA T^CCGGA AfaI 435 GTAC GT^AC AhdI 1068 GACTGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 1281 GATCC GGATC(4/5) AlwNI 932 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 384 CCTGAGG CC^T[ACGT]AGG AspEI 1068 GACTGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 463 GAGCAC G[AT]GC[AT]^C BfaI 358 CTAG C^TAG BglII 771 AGATCT A^GATCT BsaWI 906 TCCGGA [AT]^CCGG[AT] BseAI 906 TCCGGA T^CCGGA BsgI 256 CTGCAC GTGCAG(16/14) BsiHKAI 463 GAGCAC G[AT]GC[AT]^C BsmAI 766 GTCTC GTCTC(1/5) BsmBI 765 CGTCTC CGTCTC(1/5) BspEI 906 TCCGGA T^CCGGA BsrFI 1262 ACCGGC [GA]^CCGG[CT] BstAPI 1157 GCAAGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 378 GGTGACC G^GT[ACGT]ACC BstPI 378 GGTGACC G^GT[ACGT]ACC Bsu36I 384 CCTGAGG CC^T[ACGT]AGG Cfr10I 1262 ACCGGC [GA]^CCGG[CT] DrdI 1023 GACAGTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 312 CGGCCA [CT]^GGCC[GA] Eam1105I 1068 GACTGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1213 GAAGAG CTCTTC(1/4) Eco81I 384 CCTGAGG CC^T[ACGT]AGG EcoO65I 378 GGTGACC G^GT[ACGT]ACC EcoT14I 1034 CCATGG C^C[AT][AT]GG Ksp632I 1213 GAAGAG CTCTTC(1/4) KspI 820 CCGCGG CCGC^GG MaeI 358 CTAG C^TAG MroI 906 TCCGGA T^CCGGA NcoI 1034 CCATGG C^CATGG PflMI 1120 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 337 GGGACCT [GA]G^G[AT]CC[CT] PstI 8 CTGCAG CTGCA^G PvuII 552 CAGCTG CAG^CTG RsaI 435 GTAC GT^AC SacII 820 CCGCGG CCGC^GG SfcI 8 CTGCAG C^T[GA][CT]AG StyI 1034 CCATGG C^C[AT][AT]GG Tsp509I 470 AATT ^AATT Van91I 1120 CCACCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1001 CCAGCAGCCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### ApaI 31 GGGCCC GGGCC^C 847 GGGCCC BanI 976 GGCACC G^G[CT][GA]CC 1129 GGTGCC BbsI 241 GTCTTC GAAGAC(2/6) 289 GTCTTC BglI 516 GCCCAACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 968 GCCGGACAGGC BpuAI 241 GTCTTC GAAGAC(2/6) 289 GTCTTC BsaAI 139 TACGTG [CT]AC^GT[GA] 1147 TACGTG BspMI 949 ACCTGC ACCTGC(4/8) 1109 ACCTGC BstYI 771 AGATCT [GA]^GATC[CT] 1280 AGATCC DpnII 772 GATC ^GATC 1281 GATC DsaI 820 CCGCGG C^C[GA][CT]GG 1034 CCATGG MaeII 140 ACGT A^CGT 1148 ACGT MboI 772 GATC ^GATC 1281 GATC MflI 771 AGATCT [GA]^GATC[CT] 1280 AGATCC NdeII 772 GATC ^GATC 1281 GATC NlaIII 1035 CATG CATG^ 1173 CATG Sau3AI 772 GATC ^GATC 1281 GATC SexAI 374 ACCAGGT A^CC[AT]GGT 584 ACCAGGT SmaI 543 CCCGGG CCC^GGG 720 CCCGGG TaqI 245 TCGA T^CGA 605 TCGA TfiI 130 GAATC G^A[AT]TC 922 GAATC TthHB8I 245 TCGA T^CGA 605 TCGA XhoII 771 AGATCT [GA]^GATC[CT] 1280 AGATCC XmaCI 543 CCCGGG C^CCGGG 720 CCCGGG XmaI 543 CCCGGG C^CCGGG 720 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AccII AciI AluI AspI AvaI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIV PflFI PleI Sau96I ScrFI SfaNI TseI Tsp45I TspRI Tth111I