WRAP53 ORF clone pFN21AE5682

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE5682

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Clone info.

Species

human

Clone Name

pFN21AE5682 Sequence (with vector) Restriction Map

Product ID

FHC25877

ORF Sequence

DNA sequence (1644 bp)
Protein sequence (548 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE5682

Symbol

WRAP53

Alias : DKCB3, TCAB1, WDR79

Description

WD repeat containing, antisense to TP53, transcript variant 1

Original Clone ID

oc95682

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1644 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 548 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS11119.1 4.4e-134 100.0 WRAP53 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR019781 170 197 PF00400 WD40 repeat IPR019781 240 258 PF00400 WD40 repeat IPR019781 282 304 PF00400 WD40 repeat IPR019781 322 349 PF00400 WD40 repeat IPR019781 358 387 PF00400 WD40 repeat IPR019781 421 441 PF00400 WD40 repeat HMMSmart IPR001680 160 197 SM00320 WD40 repeat IPR001680 261 304 SM00320 WD40 repeat IPR001680 311 355 SM00320 WD40 repeat IPR001680 356 396 SM00320 WD40 repeat IPR001680 400 441 SM00320 WD40 repeat ProfileScan IPR017986 321 450 PS50294 WD40-repeat-containing domain IPR019782 363 398 PS50082 WD40 repeat 2 IPR019782 421 441 PS50082 WD40 repeat 2
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_060551 548 612661,613988 100.0 100.0 NP_001137462 548 612661,613988 100.0 100.0 NP_001137463 548 612661,613988 100.0 100.0 NP_001137464 548 612661,613988 100.0 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE5682 Download

>KIEE5682  1644 bp
ATGAAGACTTTGGAGACTCAACCGTTAGCTCCGGACTGCTGTCCTTCAGACCAGGACCCA
GCTCCAGCCCATCCTTCTCCCCACGCTTCCCCGATGAATAAAAATGCGGACTCTGAACTG
ATGCCACCGCCTCCCGAAAGGGGGGATCCGCCCCGGTTGTCCCCAGATCCTGTGGCTGGC
TCAGCTGTGTCCCAGGAGCTACGGGAGGGGGACCCAGTTTCTCTCTCCACTCCCCTGGAA
ACAGAGTTTGGTTCCCCTAGTGAGTTGAGTCCTCGAATCGAGGAGCAAGAACTTTCTGAA
AATACAAGCCTTCCTGCAGAAGAAGCAAACGGGAGCCTTTCTGAAGAAGAAGCGAACGGG
CCAGAGTTGGGGTCTGGAAAAGCCATGGAAGATACCTCTGGGGAACCCGCTGCAGAGGAC
GAGGGAGACACCGCTTGGAACTACAGCTTCTCCCAGCTGCCTCGATTTCTCAGTGGTTCC
TGGTCAGAGTTCAGCACCCAACCTGAGAACTTCTTGAAAGGCTGTAAGTGGGCTCCTGAC
GGTTCCTGCATCTTGACCAATAGTGCTGATAACATCTTGCGAATTTATAACCTGCCCCCA
GAGCTGTACCATGAGGGGGAGCAGGTGGAATATGCAGAAATGGTCCCTGTCCTTCGAATG
GTGGAAGGTGATACCATCTATGATTACTGCTGGTATTCTCTGATGTCCTCAGCCCAGCCA
GACACCTCCTACGTGGCCAGCAGCAGCCGGGAGAACCCGATTCATATCTGGGACGCATTC
ACTGGAGAGCTCCGGGCTTCCTTTCGCGCCTACAACCACCTGGATGAGCTGACGGCAGCC
CATTCGCTCTGCTTCTCCCCGGATGGCTCCCAGCTCTTCTGTGGCTTCAACCGGACTGTG
CGTGTTTTTTCCACGGCCCGGCCTGGCCGAGACTGCGAGGTCCGAGCCACATTTGCAAAA
AAGCAGGGCCAGAGCGGCATCATCTCCTGCATAGCCTTCAGCCCAGCCCAGCCCCTCTAT
GCCTGTGGCTCCTACGGCCGCTCCCTGGGTCTGTATGCCTGGGATGATGGCTCCCCTCTC
GCCTTGCTGGGAGGGCACCAAGGGGGCATCACCCACCTCTGCTTTCATCCCGATGGCAAC
CGCTTCTTCTCAGGAGCCCGCAAGGATGCTGAGCTCCTGTGCTGGGATCTCCGGCAGTCT
GGTTACCCACTGTGGTCCCTGGGTCGAGAGGTGACCACCAATCAGCGCATCTACTTCGAT
CTGGACCCGACCGGGCAGTTCCTAGTGAGTGGCAGCACGAGCGGGGCTGTCTCTGTGTGG
GACACGGACGGGCCTGGCAATGATGGGAAGCCGGAGCCCGTGTTGAGTTTTCTGCCCCAG
AAGGACTGCACCAATGGCGTGAGCCTGCACCCTAGCCTGCCTCTCCTGGCCACTGCCTCC
GGTCAGCGTGTGTTTCCTGAGCCCACAGAGAGTGGGGACGAAGGAGAGGAGCTGGGCCTT
CCCTTGCTCTCCACGCGCCACGTCCACCTTGAATGTCGGCTTCAGCTCTGGTGGTGTGGG
GGGGCGCCAGACTCCAGCATCCCTGATGATCACCAGGGCGAGAAAGGGCAGGGAGGAACG
GAGGGAGGTGTGGGTGAGCTGATA

ORF Protein

Cloned ORF protein sequence for pFN21AE5682 Download

>KIEE5682  548 aa
MKTLETQPLAPDCCPSDQDPAPAHPSPHASPMNKNADSELMPPPPERGDPPRLSPDPVAG
SAVSQELREGDPVSLSTPLETEFGSPSELSPRIEEQELSENTSLPAEEANGSLSEEEANG
PELGSGKAMEDTSGEPAAEDEGDTAWNYSFSQLPRFLSGSWSEFSTQPENFLKGCKWAPD
GSCILTNSADNILRIYNLPPELYHEGEQVEYAEMVPVLRMVEGDTIYDYCWYSLMSSAQP
DTSYVASSSRENPIHIWDAFTGELRASFRAYNHLDELTAAHSLCFSPDGSQLFCGFNRTV
RVFSTARPGRDCEVRATFAKKQGQSGIISCIAFSPAQPLYACGSYGRSLGLYAWDDGSPL
ALLGGHQGGITHLCFHPDGNRFFSGARKDAELLCWDLRQSGYPLWSLGREVTTNQRIYFD
LDPTGQFLVSGSTSGAVSVWDTDGPGNDGKPEPVLSFLPQKDCTNGVSLHPSLPLLATAS
GQRVFPEPTESGDEGEELGLPLLSTRHVHLECRLQLWWCGGAPDSSIPDDHQGEKGQGGT
EGGVGELI

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE5682 Download

>pFN21AE5682  6349 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAAGACTTTGGAGACTCAACCGTTAGCTCCGGA
CTGCTGTCCTTCAGACCAGGACCCAGCTCCAGCCCATCCTTCTCCCCACGCTTCCCCGAT
GAATAAAAATGCGGACTCTGAACTGATGCCACCGCCTCCCGAAAGGGGGGATCCGCCCCG
GTTGTCCCCAGATCCTGTGGCTGGCTCAGCTGTGTCCCAGGAGCTACGGGAGGGGGACCC
AGTTTCTCTCTCCACTCCCCTGGAAACAGAGTTTGGTTCCCCTAGTGAGTTGAGTCCTCG
AATCGAGGAGCAAGAACTTTCTGAAAATACAAGCCTTCCTGCAGAAGAAGCAAACGGGAG
CCTTTCTGAAGAAGAAGCGAACGGGCCAGAGTTGGGGTCTGGAAAAGCCATGGAAGATAC
CTCTGGGGAACCCGCTGCAGAGGACGAGGGAGACACCGCTTGGAACTACAGCTTCTCCCA
GCTGCCTCGATTTCTCAGTGGTTCCTGGTCAGAGTTCAGCACCCAACCTGAGAACTTCTT
GAAAGGCTGTAAGTGGGCTCCTGACGGTTCCTGCATCTTGACCAATAGTGCTGATAACAT
CTTGCGAATTTATAACCTGCCCCCAGAGCTGTACCATGAGGGGGAGCAGGTGGAATATGC
AGAAATGGTCCCTGTCCTTCGAATGGTGGAAGGTGATACCATCTATGATTACTGCTGGTA
TTCTCTGATGTCCTCAGCCCAGCCAGACACCTCCTACGTGGCCAGCAGCAGCCGGGAGAA
CCCGATTCATATCTGGGACGCATTCACTGGAGAGCTCCGGGCTTCCTTTCGCGCCTACAA
CCACCTGGATGAGCTGACGGCAGCCCATTCGCTCTGCTTCTCCCCGGATGGCTCCCAGCT
CTTCTGTGGCTTCAACCGGACTGTGCGTGTTTTTTCCACGGCCCGGCCTGGCCGAGACTG
CGAGGTCCGAGCCACATTTGCAAAAAAGCAGGGCCAGAGCGGCATCATCTCCTGCATAGC
CTTCAGCCCAGCCCAGCCCCTCTATGCCTGTGGCTCCTACGGCCGCTCCCTGGGTCTGTA
TGCCTGGGATGATGGCTCCCCTCTCGCCTTGCTGGGAGGGCACCAAGGGGGCATCACCCA
CCTCTGCTTTCATCCCGATGGCAACCGCTTCTTCTCAGGAGCCCGCAAGGATGCTGAGCT
CCTGTGCTGGGATCTCCGGCAGTCTGGTTACCCACTGTGGTCCCTGGGTCGAGAGGTGAC
CACCAATCAGCGCATCTACTTCGATCTGGACCCGACCGGGCAGTTCCTAGTGAGTGGCAG
CACGAGCGGGGCTGTCTCTGTGTGGGACACGGACGGGCCTGGCAATGATGGGAAGCCGGA
GCCCGTGTTGAGTTTTCTGCCCCAGAAGGACTGCACCAATGGCGTGAGCCTGCACCCTAG
CCTGCCTCTCCTGGCCACTGCCTCCGGTCAGCGTGTGTTTCCTGAGCCCACAGAGAGTGG
GGACGAAGGAGAGGAGCTGGGCCTTCCCTTGCTCTCCACGCGCCACGTCCACCTTGAATG
TCGGCTTCAGCTCTGGTGGTGTGGGGGGGCGCCAGACTCCAGCATCCCTGATGATCACCA
GGGCGAGAAAGGGCAGGGAGGAACGGAGGGAGGTGTGGGTGAGCTGATAGTTTAAACGAA
TTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGG
CTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAG
CATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGA
CAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATT
GCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT
TTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTAC
AAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAG
ATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTAC
GTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATT
TGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAA
ATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTT
ATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA
GTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC
AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTC
AAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT
CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCAT
CTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAAC
ACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG
CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCC
ATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAA
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG
GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCT
GATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT
GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA
CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATG
ACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCA
CAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGA
ACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC
ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG
CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGAT
ACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT
ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTC
AGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACG
ACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCG
GTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTG
GTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCG
GCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCA
GAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGA
ACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGA
TCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGA
AACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTG
GTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGT
GGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTG
CGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATA
AACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT