################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII Acc65I AccI AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BbrPI BciVI BfrI BglII BlnI BsaI BsiWI BsmBI BsoBI Bsp1407I BspDI BspHI BspLU11I BsrFI BsrGI BssHII Bst1107I BstAPI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII Eam1105I Eco47III Eco81I EcoNI EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KpnI KspI MfeI MluI MseI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccIII 30 TCCGGA T^CCGGA AcsI 581 GAATTT [GA]^AATT[CT] AcyI 1563 GGCGCC G[GA]^CG[CT]C AfaI 606 GTAC GT^AC AlwNI 164 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 581 GAATTT [GA]^AATT[CT] BamHI 144 GGATCC G^GATCC BbeI 1563 GGCGCC GGCGC^C BbsI 3 GAAGAC GAAGAC(2/6) BbvCI 707 CCTCAGC CCTCAGC(-5/-2) BclI 1587 TGATCA T^GATCA BglI 968 GCCAGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 3 GAAGAC GAAGAC(2/6) BsaAI 730 TACGTG [CT]AC^GT[GA] BsaBI 759 GATTCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1563 GGCGCC G[GA]^CG[CT]C BseAI 30 TCCGGA T^CCGGA BsmI 775 GCATTC GAATGC(1/-1) BspEI 30 TCCGGA T^CCGGA BsrDI 1337 GCAATG GCAATG(2/0) BssSI 1296 CACGAG CACGAG(-5/-1) BstBI 653 TTCGAA TT^CGAA BstXI 227 CCACTCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DrdI 931 GACTGCGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1035 CGGCCG C^GGCCG EarI 874 CTCTTC CTCTTC(1/4) EclXI 1035 CGGCCG C^GGCCG Eco52I 1035 CGGCCG C^GGCCG FbaI 1587 TGATCA T^GATCA HaeII 1563 GGCGCC [GA]GCGC^[CT] HgaI 772 GACGC GACGC(5/10) Hin1I 1563 GGCGCC G[GA]^CG[CT]C KasI 1563 GGCGCC G^GCGCC Ksp632I 874 CTCTTC CTCTTC(1/4) MamI 759 GATTCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 30 TCCGGA T^CCGGA NarI 1563 GGCGCC GG^CGCC NcoI 383 CCATGG C^CATGG NspV 653 TTCGAA TT^CGAA PshAI 34 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsaI 606 GTAC GT^AC SapI 873 GCTCTTC GCTCTTC(1/4) SfoI 1563 GGCGCC GGC^GCC SfuI 653 TTCGAA TT^CGAA Tsp45I 1231 GTGAC ^GT[GC]AC Tsp509I 582 AATT ^AATT XcmI 870 CCAGCTCTTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccII 805 CGCG CG^CG 1514 CGCG AspHI 787 GAGCTC G[AT]GC[AT]^C 1171 GAGCTC BalI 734 TGGCCA TGG^CCA 1427 TGGCCA BanI 1094 GGCACC G^G[CT][GA]CC 1563 GGCGCC BcgI 835 GCAGCCCATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1247 GCATCTACTTCG BlpI 179 GCTCAGC GC^T[ACGT]AGC 1168 GCTGAGC Bpu1102I 179 GCTCAGC GC^T[ACGT]AGC 1168 GCTGAGC BseRI 280 GAGGAG GAGGAG(10/8) 1486 GAGGAG BsgI 1386 CTGCAC GTGCAG(16/14) 1405 CTGCAC BsiEI 1035 CGGCCG CG[GA][CT]^CG 1268 CGACCG BsiHKAI 787 GAGCTC G[AT]GC[AT]^C 1171 GAGCTC BspMI 590 ACCTGC ACCTGC(4/8) 621 GCAGGT BsrI 781 ACTGG ACTGG(1/-1) 214 CCAGT BstEII 1201 GGTTACC G^GT[ACGT]ACC 1230 GGTGACC BstPI 1201 GGTTACC G^GT[ACGT]ACC 1230 GGTGACC BstUI 805 CGCG CG^CG 1514 CGCG CelII 179 GCTCAGC GC^T[ACGT]AGC 1168 GCTGAGC DraII 53 AGGACCC [GA]G^G[ACGT]CC[CT] 209 GGGACCC DsaI 383 CCATGG C^C[GA][CT]GG 911 CCACGG EcoO109I 53 AGGACCC [GA]G^G[ACGT]CC[CT] 209 GGGACCC EcoO65I 1201 GGTTACC G^GT[ACGT]ACC 1230 GGTGACC EcoT14I 383 CCATGG C^C[AT][AT]GG 1098 CCAAGG MaeII 731 ACGT A^CGT 1520 ACGT MaeIII 1202 GTTAC ^GT[ACGT]AC 1231 GTGAC MluNI 734 TGGCCA TGG^CCA 1427 TGGCCA MscI 734 TGGCCA TGG^CCA 1427 TGGCCA MslI 817 CACCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1126 CATCCCGATG MvnI 805 CGCG CG^CG 1514 CGCG NlaIII 384 CATG CATG^ 610 CATG PpuMI 53 AGGACCC [GA]G^G[AT]CC[CT] 209 GGGACCC PstI 314 CTGCAG CTGCA^G 410 CTGCAG PvuII 182 CAGCTG CAG^CTG 454 CAGCTG SacI 787 GAGCTC GAGCT^C 1171 GAGCTC StyI 383 CCATGG C^C[AT][AT]GG 1098 CCAAGG TfiI 275 GAATC G^A[AT]TC 759 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AciI AluI AlwI AvaII BanII BbvI BcnI BfaI BmyI BpmI BsaJI BsaWI BsiYI BslI BsmAI BsmFI Bsp1286I BsrBI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII EaeI EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TseI TspRI TthHB8I XhoII