Restriction map for
pFN21AE5682
(Circular) (Six-base) MAPSORT of: pFN21AE5682.seq Check: 6773 from: 1 to: 6349
pFN21AE5682 6349 bp
With 182 enzymes: SgfI *
December 8, 2014 13:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2617 2617
Size: 6349
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4159 278
Size: 53 83 186 936 2623 2468
Fragments arranged by size:
2623 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3692 1937
Size: 1755 4594
Acc65I G'GTAC_C
Cuts at: 3670 3670
Size: 6349
AclI AA'CG_TT
Cuts at: 1563 4476 4849 1563
Size: 2913 373 3063
Fragments arranged by size:
3063 2913 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2034 2367 2986 3530 4412 5796 6064 1632
Size: 402 333 619 544 882 1384 268 1917
Fragments arranged by size:
1917 1384 882 619 544 402 333 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6152
AflIII A'CryG_T
Cuts at: 1203 5248 6225 1203
Size: 4045 977 1327
Fragments arranged by size:
4045 1327 977
AgeI A'CCGG_T
Cuts at: 5180 5180
Size: 6349
AhdI GACnn_n'nnGTC
Cuts at: 5078 5078
Size: 6349
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6349
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6349
AlwNI CAG_nnn'CTG
Cuts at: 2174 4092 5664 2174
Size: 1918 1572 2859
Fragments arranged by size:
2859 1918 1572
ApaLI G'TGCA_C
Cuts at: 4406 5562 4406
Size: 1156 5193
ApoI r'AATT_y
Cuts at: 1493 2586 3658 3884 4036 6145 1493
Size: 1093 1072 226 152 2109 1697
Fragments arranged by size:
2109 1697 1093 1072 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4903 160
Size: 872 3871 1606
Fragments arranged by size:
3871 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6349
AvaI C'yCGr_G
Cuts at: 1870 1943 3674 1870
Size: 73 1731 4545
Fragments arranged by size:
4545 1731 73
BamHI G'GATC_C
Cuts at: 2149 3679 4046 2149
Size: 1530 367 4452
Fragments arranged by size:
4452 1530 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3099 3568 3670 5125 618
Size: 325 217 627 1312 469 102 1455 1842
Fragments arranged by size:
1842 1455 1312 627 469 325 217 102
BanII G_rGCy'C
Cuts at: 1391 2539 2796 3163 3180 3363 3468 3668 1391
Size: 1148 257 367 17 183 105 200 4072
Fragments arranged by size:
4072 1148 367 257 200 183 105 17
BbeI G_GCGC'C
Cuts at: 3572 3572
Size: 6349
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2015 928
Size: 971 116 5262
Fragments arranged by size:
5262 971 116
BbvCI CC'TCA_GC
Cuts at: 2713 2713
Size: 6349
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2829 2863 3241 3275 4540 4574 1903
Size: 34 892 34 378 34 1265 34 3678
Fragments arranged by size:
3678 1265 892 378 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4241 5451 4241
Size: 1210 5139
BclI T'GATC_A
Cuts at: 1548 3592 1548
Size: 2044 4305
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2979 4960 136
Size: 107 122 71 631 605 1307 1981 1525
Fragments arranged by size:
1981 1525 1307 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6344 6344
Size: 6349
BlpI GC'TnA_GC
Cuts at: 2185 3174 3766 2185
Size: 989 592 4768
Fragments arranged by size:
4768 989 592
Bme1580I G_kGCm'C
Cuts at: 1790 3102 4410 5566 1790
Size: 1312 1308 1156 2573
Fragments arranged by size:
2573 1312 1308 1156
BmgBI CAC'GTC
Cuts at: 1318 3526 1318
Size: 2208 4141
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2213 4080 5038
Size: 416 53 83 399 24 796 1867 958
Cuts at: 5038 442
Size: 1753
Fragments arranged by size:
1867 1753 958 796 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6349
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2052 2808 3562 5009 1239
Size: 813 756 754 1447 2579
Fragments arranged by size:
2579 1447 813 756 754
Bpu10I CC'TnA_GC
Cuts at: 2713 3462 2713
Size: 749 5600
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4474 5339 5637 4474
Size: 865 298 5186
Fragments arranged by size:
5186 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5012 882
Size: 617 3513 2219
Fragments arranged by size:
3513 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2737 4140 493
Size: 711 1533 1403 2702
Fragments arranged by size:
2702 1533 1403 711
BsaBI GATnn'nnATC
Cuts at: 2768 6343 2768
Size: 3575 2774
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3569 4156 4538 5173
Size: 53 83 186 936 2036 587 382 635
Cuts at: 5173 275
Size: 1451
Fragments arranged by size:
2036 1451 936 635 587 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2035 2895 3443 4782 5180 5454 5601 6012 2035
Size: 860 548 1339 398 274 147 411 2372
Fragments arranged by size:
2372 1339 860 548 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2438 2468 3029 3059 3350
Size: 30 165 30 1035 30 561 30 291
Cuts at: 3350 3380 1178
Size: 30 4147
Fragments arranged by size:
4147 1035 561 291 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2300 3506 1186
Size: 1114 1206 4029
Fragments arranged by size:
4029 1206 1114
BseYI C'CCAG_C
Cuts at: 2062 2457 2718 2874 3007 3012 3091 3186 3496
Size: 395 261 156 133 5 79 95 310
Cuts at: 3496 5552 6048 2062
Size: 2056 496 2363
Fragments arranged by size:
2363 2056 496 395 310 261 156 133
95 79 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3376 3395 1163
Size: 573 1640 19 4117
Fragments arranged by size:
4117 1640 573 19
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3043 3276 3799 4560 5588 1540
Size: 275 186 1042 233 523 761 1028 2301
Fragments arranged by size:
2301 1042 1028 761 523 275 233 186
BsiHKAI G_wGCw'C
Cuts at: 2796 3180 4410 4495 5566 2796
Size: 384 1230 85 1071 3579
Fragments arranged by size:
3579 1230 1071 384 85
BsmI GAATG_Cn'
Cuts at: 2780 3860 3953 2780
Size: 1080 93 5176
Fragments arranged by size:
5176 1080 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2539 2796 3102 3163 3180 3363 3468
Size: 399 749 257 306 61 17 183 105
Cuts at: 3468 3668 4410 4495 5566 1391
Size: 200 742 85 1071 2174
Fragments arranged by size:
2174 1071 749 742 399 306 257 200
183 105 85 61 17
BspEI T'CCGG_A
Cuts at: 2035 6012 2035
Size: 3977 2372
BspHI T'CATG_A
Cuts at: 4238 5968 4238
Size: 1730 4619
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2604 2617 3704 844
Size: 1760 13 1087 3489
Fragments arranged by size:
3489 1760 1087 13
BsrBI CCG'CTC
Cuts at: 1394 1823 2979 3045 3306 4236 1394
Size: 429 1156 66 261 930 3507
Fragments arranged by size:
3507 1156 930 429 261 66
BsrDI GCAATG_nn'
Cuts at: 65 1248 3349 4844 5018 65
Size: 1183 2101 1495 174 1396
Fragments arranged by size:
2101 1495 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4993 5180 1700
Size: 110 3183 187 2869
Fragments arranged by size:
3183 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6349
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6349
BssSI C'ACGA_G
Cuts at: 3301 4409 5421 3301
Size: 1108 1012 4229
Fragments arranged by size:
4229 1108 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6349
BstBI TT'CG_AA
Cuts at: 2659 5153 2659
Size: 2494 3855
BstEII G'GTnAC_C
Cuts at: 1184 3206 3235 1184
Size: 2022 29 4298
Fragments arranged by size:
4298 2022 29
BstXI CCAn_nnnn'nTGG
Cuts at: 2239 2239
Size: 6349
BstYI r'GATC_y
Cuts at: 1972 2149 2170 3190 3679 4046 4431 4448 5900
Size: 177 21 1020 489 367 385 17 1452
Cuts at: 5900 5986 5998 6344 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1020 489 385 367 346 177
86 21 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2388 2916 513
Size: 552 1323 528 3946
Fragments arranged by size:
3946 1323 552 528
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5610
Fragments arranged by size:
5610 683 56
BtsI GCAGTG_nn'
Cuts at: 3435 3865 4659 4679 3435
Size: 430 794 20 5105
Fragments arranged by size:
5105 794 430 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6349
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6314
DraI TTT'AAA
Cuts at: 3653 3653
Size: 6349
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2942 5356 809
Size: 507 1626 2414 1802
Fragments arranged by size:
2414 1802 1626 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2739 2929 3040
Size: 54 1425 50 275 18 909 190 111
Cuts at: 3040 3432 3796 4685 6314 8
Size: 392 364 889 1629 43
Fragments arranged by size:
1629 1425 909 889 392 364 275 190
111 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3040 3796 1537
Size: 275 1228 756 4090
Fragments arranged by size:
4090 1228 756 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2885 4279 1353
Size: 1532 1394 3423
Fragments arranged by size:
3423 1532 1394
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2142 4936 5310 5456 387
Size: 1064 138 553 2794 374 146 1280
Fragments arranged by size:
2794 1280 1064 553 374 146 138
EcoICRI GAG'CTC
Cuts at: 2794 3178 2794
Size: 384 5965
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2034 2052 2367 2808 2986 3530 3562
Size: 393 402 18 315 441 178 544 32
Cuts at: 3562 4412 5009 5796 6064 1239
Size: 850 597 787 268 1524
Fragments arranged by size:
1524 850 787 597 544 441 402 393
315 268 178 32 18
EcoO109I rG'GnC_Cy
Cuts at: 2059 2215 2059
Size: 156 6193
EcoRI G'AATT_C
Cuts at: 3658 4036 3658
Size: 378 5971
FspI TGC'GCA
Cuts at: 4855 4855
Size: 6349
HaeII r_GCGC'y
Cuts at: 1678 3572 5496 1678
Size: 1894 1924 2531
Fragments arranged by size:
2531 1924 1894
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2360 2392 3059 3091 3229
Size: 32 135 32 788 32 667 32 138
Cuts at: 3229 3261 4990 5022 5064 5096 1373
Size: 32 1729 32 42 32 2626
Fragments arranged by size:
2626 1729 788 667 138 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3693 3939 669
Size: 988 281 1755 246 3079
Fragments arranged by size:
3079 1755 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6349
HpaI GTT'AAC
Cuts at: 3939 3939
Size: 6349
KasI G'GCGC_C
Cuts at: 3568 3568
Size: 6349
KpnI G_GTAC'C
Cuts at: 3674 3674
Size: 6349
MfeI C'AATT_G
Cuts at: 3948 3948
Size: 6349
MluI A'CGCG_T
Cuts at: 6225 6225
Size: 6349
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5463 5647 1305
Size: 4158 184 2007
Fragments arranged by size:
4158 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2741 3434 10
Size: 54 1425 343 909 693 2925
Fragments arranged by size:
2925 1425 909 693 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2826 3135 4307 4666 4825 518
Size: 648 1660 309 1172 359 159 2042
Fragments arranged by size:
2042 1660 1172 648 359 309 159
MspA1I CmG'CkG
Cuts at: 1606 2189 2414 2461 3765 4442 5590 5835 1606
Size: 583 225 47 1304 677 1148 245 2120
Fragments arranged by size:
2120 1304 1148 677 583 245 225 47
NarI GG'CG_CC
Cuts at: 3569 3569
Size: 6349
NcoI C'CATG_G
Cuts at: 513 1065 2388 513
Size: 552 1323 4474
Fragments arranged by size:
4474 1323 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6349
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6349
NotI GC'GGCC_GC
Cuts at: 3796 3796
Size: 6349
NspI r_CATG'y
Cuts at: 1132 1229 3707 5252 1132
Size: 97 2478 1545 2229
Fragments arranged by size:
2478 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3049 3223 1348
Size: 1701 174 4474
Fragments arranged by size:
4474 1701 174
PciI A'CATG_T
Cuts at: 5248 5248
Size: 6349
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6349
PmeI GTTT'AAAC
Cuts at: 3653 3653
Size: 6349
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4418 4450 5931 5963 4418
Size: 32 1481 32 4804
Fragments arranged by size:
4804 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2059 2215 2059
Size: 156 6193
PshAI GACnn'nnGTC
Cuts at: 1613 2043 1613
Size: 430 5919
PsiI TTA'TAA
Cuts at: 2592 3919 2592
Size: 1327 5022
PstI C_TGCA'G
Cuts at: 830 2323 2419 3701 830
Size: 1493 96 1282 3478
Fragments arranged by size:
3478 1493 1282 96
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6349
PvuII CAG'CTG
Cuts at: 2189 2461 2189
Size: 272 6077
SacI G_AGCT'C
Cuts at: 2796 3180 2796
Size: 384 5965
SalI G'TCGA_C
Cuts at: 1936 3691 1936
Size: 1755 4594
SanDI GG'GwC_CC
Cuts at: 2215 2215
Size: 6349
SapI GCTCTTCn'nnn_
Cuts at: 2885 2885
Size: 6349
SbfI CC_TGCA'GG
Cuts at: 3701 3701
Size: 6349
ScaI AGT'ACT
Cuts at: 4597 6309 4597
Size: 1712 4637
SfcI C'TryA_G
Cuts at: 826 1045 2319 2415 2446 3697 4832 5513 5704
Size: 219 1274 96 31 1251 1135 681 191
Cuts at: 5704 826
Size: 1471
Fragments arranged by size:
1471 1274 1251 1135 681 219 191 96
31
SfoI GGC'GCC
Cuts at: 3570 3570
Size: 6349
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6349
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6349
SmaI CCC'GGG
Cuts at: 1872 3676 1872
Size: 1804 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4453 5354 5616 820
Size: 197 926 2510 901 262 1553
Fragments arranged by size:
2510 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4140 493
Size: 3647 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6349
SphI G_CATG'C
Cuts at: 1132 3707 1132
Size: 2575 3774
SspI AAT'ATT
Cuts at: 5 52 4273 5
Size: 47 4221 2081
Fragments arranged by size:
4221 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6349
StyI C'CwwG_G
Cuts at: 513 1065 2388 3103 3788 513
Size: 552 1323 715 685 3074
Fragments arranged by size:
3074 1323 715 685 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2516 3131 3626 4376 4393 4546 4731
Size: 1255 936 615 495 750 17 153 185
Cuts at: 4731 5176 325
Size: 445 1498
Fragments arranged by size:
1498 1255 936 750 615 495 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4595 6307 96
Size: 274 80 33 51 1443 2618 1712 138
Fragments arranged by size:
2618 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2890 2922 6309 6341 2890
Size: 32 3387 32 2898
Fragments arranged by size:
3387 2898 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6349
XbaI T'CTAG_A
Cuts at: 3685 3685
Size: 6349
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2882 2011
Size: 871 5478
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6349
XmaI C'CCGG_G
Cuts at: 1870 3674 1870
Size: 1804 4545
XmnI GAAnn'nnTTC
Cuts at: 4478 4478
Size: 6349
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4157 276
Size: 53 83 186 936 2623 2468
Fragments arranged by size:
2623 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI
SrfI SwaI