HACD1 ORF clone pFN21AE5314

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Halotag ORF Clone pFN21AE5314

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Clone info.

Species

human

Clone Name

pFN21AE5314 Sequence (with vector) Restriction Map

Product ID

FHC23783

ORF Sequence

DNA sequence (864 bp)
Protein sequence (288 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE5314

Symbol

HACD1

Alias : CAP, PTPLA

Description

3-hydroxyacyl-CoA dehydratase 1

Original Clone ID

oc95314

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 864 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 288 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS7121.1 3.2e-110 99.3 HACD1 CCDS46895.1 5.7e-60 62.1 HACD2 CCDS45282.1 5.9e-16 27.8 HACD3 CCDS43791.1 4.1e-12 23.8 HACD4 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR007482 119 279 PF04387 Protein-tyrosine phosphatase-like
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 72 ATAWLTFYDIAMTAGWLVLAIAM 94 PRIMARY 23 2 113 KTLKFFQTFALLEIVHCLIGIVP 135 SECONDARY 23 3 139 IVTGVQVSSRIFMVWLITHSIKP 161 SECONDARY 23 4 164 NEESVVLFLVAWTVTEITRYSFY 186 SECONDARY 23 5 204 NFFIILYPVGVAGELLTIYAALP 226 SECONDARY 23 6 249 YYFLLITMASYIPLFPQLYFHML 271 SECONDARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_055056 288 610467 99.3 100.0 NP_940684 254 615939 62.1 85.1 XP_005252698 252 610467 86.8 100.0 NP_001316712 277 615939 56.8 85.1 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE5314 Download

>KIEE5314  864 bp
ATGGGGCGCCTGACGGAAGCGGCGGCAGCGGGCAGCGGCTCTCGGGCTGCAGGCTGGGCA
GGGTCCCCTCCCACGCTCCTGCCGCTGTCTCCCACGTCCCCCAGGTGCGCGGCCACCATG
GCGTCCAGCGACGAGGACGGCACCAACGGCGGCGCCTCGGAGGCCGGCGAGGACCGGGAG
GCTCCCGGCAAGCGGAGGCGCCTGGGGTTCTTGGCCACCGCCTGGCTCACCTTCTACGAC
ATCGCCATGACCGCGGGGTGGTTGGTTCTAGCTATTGCCATGGTACGTTTTTATATGGAA
AAAGGAACACACAGAGGTTTATATAAAAGTATTCAGAAGACACTTAAATTTTTCCAGACA
TTTGCCTTGCTTGAGATAGTTCACTGTTTAATTGGAATTGTACCTACTTCTGTGATTGTG
ACTGGGGTCCAAGTGAGTTCAAGAATCTTTATGGTGTGGCTCATTACTCACAGTATAAAA
CCAATCCAGAATGAAGAGAGTGTGGTGCTTTTTCTGGTCGCGTGGACTGTGACAGAGATC
ACTCGCTATTCCTTCTACACATTCAGCCTTCTTGACCACTTGCCATACTTCATTAAATGG
GCCAGATATAATTTTTTTATCATCTTATATCCTGTTGGAGTTGCTGGTGAACTTCTTACA
ATATACGCTGCCTTGCCGCATGTGAAGAAAACAGGAATGTTTTCAATAAGACTTCCTAAC
AAATACAATGTCTCTTTTGACTACTATTATTTTCTTCTTATAACCATGGCATCATATATA
CCTTTGTTTCCACAACTCTATTTTCATATGTTACGTCAAAGAAGAAAGGTGCTTCATGGA
GAGGTGATTGTAGAAAAGGATGAT

ORF Protein

Cloned ORF protein sequence for pFN21AE5314 Download

>KIEE5314  288 aa
MGRLTEAAAAGSGSRAAGWAGSPPTLLPLSPTSPRCAATMASSDEDGTNGGASEAGEDRE
APGKRRRLGFLATAWLTFYDIAMTAGWLVLAIAMVRFYMEKGTHRGLYKSIQKTLKFFQT
FALLEIVHCLIGIVPTSVIVTGVQVSSRIFMVWLITHSIKPIQNEESVVLFLVAWTVTEI
TRYSFYTFSLLDHLPYFIKWARYNFFIILYPVGVAGELLTIYAALPHVKKTGMFSIRLPN
KYNVSFDYYYFLLITMASYIPLFPQLYFHMLRQRRKVLHGEVIVEKDD

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE5314 Download

>pFN21AE5314  5569 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGGGCGCCTGACGGAAGCGGCGGCAGCGGGCAG
CGGCTCTCGGGCTGCAGGCTGGGCAGGGTCCCCTCCCACGCTCCTGCCGCTGTCTCCCAC
GTCCCCCAGGTGCGCGGCCACCATGGCGTCCAGCGACGAGGACGGCACCAACGGCGGCGC
CTCGGAGGCCGGCGAGGACCGGGAGGCTCCCGGCAAGCGGAGGCGCCTGGGGTTCTTGGC
CACCGCCTGGCTCACCTTCTACGACATCGCCATGACCGCGGGGTGGTTGGTTCTAGCTAT
TGCCATGGTACGTTTTTATATGGAAAAAGGAACACACAGAGGTTTATATAAAAGTATTCA
GAAGACACTTAAATTTTTCCAGACATTTGCCTTGCTTGAGATAGTTCACTGTTTAATTGG
AATTGTACCTACTTCTGTGATTGTGACTGGGGTCCAAGTGAGTTCAAGAATCTTTATGGT
GTGGCTCATTACTCACAGTATAAAACCAATCCAGAATGAAGAGAGTGTGGTGCTTTTTCT
GGTCGCGTGGACTGTGACAGAGATCACTCGCTATTCCTTCTACACATTCAGCCTTCTTGA
CCACTTGCCATACTTCATTAAATGGGCCAGATATAATTTTTTTATCATCTTATATCCTGT
TGGAGTTGCTGGTGAACTTCTTACAATATACGCTGCCTTGCCGCATGTGAAGAAAACAGG
AATGTTTTCAATAAGACTTCCTAACAAATACAATGTCTCTTTTGACTACTATTATTTTCT
TCTTATAACCATGGCATCATATATACCTTTGTTTCCACAACTCTATTTTCATATGTTACG
TCAAAGAAGAAAGGTGCTTCATGGAGAGGTGATTGTAGAAAAGGATGATGTTTAAACGAA
TTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGG
CTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAG
CATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGA
CAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATT
GCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCAT
TTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTAC
AAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAG
ATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTAC
GTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATT
TGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAA
ATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTT
ATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAA
GTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC
AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTC
AAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGT
CGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCAT
CTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAAC
ACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG
CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCC
ATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAA
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAG
GCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCT
GATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT
GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAA
CGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATG
ACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCA
CAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGA
ACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATC
ACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG
CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGAT
ACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGT
ATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTC
AGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACG
ACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCG
GTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTG
GTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCG
GCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCA
GAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGA
ACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGA
TCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGA
AACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTG
GTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGT
GGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTG
CGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATA
AACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT