Restriction map for
pFN21AE5314
##################################
# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AviII AvrII
BamHI BanII BbrPI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DdeI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI MamI MfeI
MflI MluI MroI MunI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tth111I TthHB8I XbaI
XcmI XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AcsI 346 AAATTT [GA]^AATT[CT]
AluI 270 AGCT AG^CT
ApoI 346 AAATTT [GA]^AATT[CT]
Asp700I 695 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 41 CTCGGG C^[CT]CG[GA]G
BalI 212 TGGCCA TGG^CCA
BbsI 336 GAAGAC GAAGAC(2/6)
BfaI 268 CTAG C^TAG
BpuAI 336 GAAGAC GAAGAC(2/6)
BsoBI 41 CTCGGG C^[CT]CG[GA]G
BsrFI 163 GCCGGC [GA]^CCGG[CT]
BsrI 421 ACTGG ACTGG(1/-1)
BstF5I 858 GGATG GGATG(2/0)
Cfr10I 163 GCCGGC [GA]^CCGG[CT]
DpnII 537 GATC ^GATC
DraII 61 GGGTCCC [GA]G^G[ACGT]CC[CT]
EarI 493 GAAGAG CTCTTC(1/4)
EcoO109I 61 GGGTCCC [GA]G^G[ACGT]CC[CT]
FokI 858 GGATG GGATG(9/13)
HgaI 121 GCGTC GACGC(5/10)
HinfI 443 GAATC G^A[ACGT]TC
Ksp632I 493 GAAGAG CTCTTC(1/4)
KspI 251 CCGCGG CCGC^GG
MaeI 268 CTAG C^TAG
MboI 537 GATC ^GATC
MluNI 212 TGGCCA TGG^CCA
MscI 212 TGGCCA TGG^CCA
MslI 240 CATCGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 163 GCCGGC GCC^GGC
NdeI 805 CATATG CA^TATG
NdeII 537 GATC ^GATC
NgoMIV 163 GCCGGC G^CCGGC
NspI 678 GCATGT [GA]CATG^[CT]
PflMI 215 CCACCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 61 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 420 GACTGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 47 CTGCAG CTGCA^G
SacII 251 CCGCGG CCGC^GG
Sau3AI 537 GATC ^GATC
SfaNI 769 GCATC GCATC(5/9)
SfcI 47 CTGCAG C^T[GA][CT]AG
SmlI 370 CTTGAG C^T[CT][GA]AG
TfiI 443 GAATC G^A[AT]TC
TspRI 382 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 215 CCACCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 695 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AfaI 283 GTAC GT^AC
400 GTAC
BcnI 174 CCGGG CC^[GC]GG
184 CCCGG
BglI 112 GCCACCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
154 GCCTCGGAGGC
BsmAI 87 GTCTC GTCTC(1/5)
730 GTCTC
BsmFI 63 GTCCC GGGAC(10/14)
96 GTCCC
EaeI 110 CGGCCA [CT]^GGCC[GA]
212 TGGCCA
FauI 28 GCGGG CCCGC(4/6)
253 GCGGG
NciI 174 CCGGG CC^[GC]GG
184 CCCGG
RsaI 283 GTAC GT^AC
400 GTAC
Tsp45I 418 GTGAC ^GT[GC]AC
529 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AcyI AvaII BanI
BbeI BbvI BsaHI BsaJI BsiYI
BslI BssKI BstNI BstUI Cac8I
CfoI Cfr13I DsaI EcoRII EcoT14I
Fnu4HI HaeII HaeIII HapII HhaI
Hin1I HinP1I HpaII HphI ItaI
KasI MaeII MaeIII MboII MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NarI NcoI NlaIII NlaIV
Sau96I ScrFI SfoI StyI Tru9I
TseI Tsp509I