LCP1 ORF clone pFN21AE4543

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Halotag ORF Clone pFN21AE4543

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Clone info.

Species

human

Clone Name

pFN21AE4543 Sequence (with vector) Restriction Map

Product ID

FHC08338

ORF Sequence

DNA sequence (1881 bp)
Protein sequence (627 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE4543

Symbol

LCP1

Alias : CP64, HEL-S-37, L-PLASTIN, LC64P, LPL, PLS2

Description

lymphocyte cytosolic protein 1 (L-plastin)

Original Clone ID

oc94543

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1881 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 627 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS9403.1 0 99.8 LCP1 CCDS14568.1 1.2e-196 79.9 PLS3 CCDS3125.1 9.5e-183 74.0 PLS1 CCDS78499.1 6.3e-105 79.5 PLS3 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR018248 13 39 PF00036 EF-hand IPR001715 122 234 PF00307 Calponin homology domain IPR001715 266 375 PF00307 Calponin homology domain IPR001715 396 502 PF00307 Calponin homology domain IPR001715 519 621 PF00307 Calponin homology domain HMMSmart IPR002048 13 41 SM00054 Calcium-binding EF-hand IPR002048 53 81 SM00054 Calcium-binding EF-hand IPR001715 122 234 SM00033 Calponin homology domain IPR001715 266 373 SM00033 Calponin homology domain IPR001715 396 501 SM00033 Calponin homology domain IPR001715 517 622 SM00033 Calponin homology domain ProfileScan IPR018249 9 44 PS50222 EF-HAND 2 IPR018249 49 84 PS50222 EF-HAND 2 IPR001715 120 236 PS50021 Calponin homology domain IPR001715 264 375 PS50021 Calponin homology domain IPR001715 394 503 PS50021 Calponin homology domain IPR001715 515 624 PS50021 Calponin homology domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_005266431 627 153430 99.8 100.0 NP_002289 627 153430 99.8 100.0 NP_001129497 630 300131,300910 79.9 100.0 NP_005023 630 300131,300910 79.9 100.0 XP_011535836 630 300131,300910 79.9 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE4543 Download

>KIEE4543  1881 bp
ATGGCCAGAGGATCAGTGTCCGATGAGGAAATGATGGAGCTCAGAGAAGCTTTTGCCAAA
GTTGATACTGATGGCAATGGATACATCAGCTTCAATGAGTTGAATGACTTGTTCAAGGCT
GCTTGCTTGCCTTTGCCTGGGTATAGAGTACGAGAAATTACAGAAAACCTGATGGCTACA
GGTGATCTGGACCAAGATGGAAGGATCAGCTTTGATGAGTTTATCAAGATTTTCCATGGC
CTAAAAAGCACAGATGTTGCCAAGACCTTTAGAAAAGCAATCAATAAGAAGGAAGGGATT
TGTGCAATCGGTGGTACTTCAGAGCAGTCTAGCGTTGGCACCCAACACTCCTATTCAGAG
GAAGAAAAGTATGCCTTTGTCAACTGGATAAACAAAGCCCTGGAAAATGATCCTGATTGT
CGGCATGTCATCCCAATGAACCCAAACACGAATGATCTCTTTAATGCTGTTGGAGATGGC
ATTGTCCTTTGTAAAATGATCAACCTGTCAGTGCCAGACACAATTGATGAAAGAACAATC
AACAAAAAGAAGCTAACCCCTTTCACCATTCAGGAAAATCTGAACTTGGCTCTGAACTCT
GCCTCAGCCATCGGGTGCCATGTGGTCAACATAGGGGCTGAGGACCTGAAGGAGGGGAAG
CCTTATCTGGTCCTGGGACTTCTGTGGCAAGTCATCAAGATTGGGTTGTTTGCTGACATT
GAACTCAGCAGAAATGAAGCTCTGATTGCTCTTTTGAGAGAAGGTGAGAGCCTGGAGGAT
TTGATGAAACTCTCCCCTGAAGAGCTCTTGCTGAGGTGGGCTAATTACCACCTGGAAAAT
GCAGGCTGCAACAAAATTGGCAACTTCAGTACTGACATCAAGGACTCAAAAGCTTATTAC
CACCTGCTTGAGCAGGTGGCTCCAAAAGGAGATGAAGAAGGTGTTCCTGCTGTTGTTATT
GACATGTCAGGACTGCGGGAGAAGGATGACATCCAGAGGGCAGAATGCATGCTGCAGCAG
GCGGAGAGGCTGGGCTGCCGGCAGTTTGTCACAGCCACAGATGTTGTCCGAGGGAACCCC
AAGTTGAACTTGGCTTTTATTGCCAACCTCTTTAACAGATACCCTGCCCTGCACAAACCA
GAGAACCAGGACATTGACTGGGGGGCTCTTGAAGGTGAGACGAGAGAAGAGCGGACATTT
AGGAACTGGATGAACTCCCTGGGTGTTAACCCTCGAGTCAATCATTTGTACAGTGACTTA
TCAGATGCCCTGGTCATCTTCCAGCTCTATGAAAAGATCAAAGTTCCTGTTGACTGGAAC
AGAGTAAACAAACCGCCATACCCCAAACTGGGAGGCAATATGAAGAAGCTTGAGAATTGT
AACTACGCGGTAGAATTGGGGAAGAATCAAGCGAAGTTCTCCCTGGTTGGCATCGGTGGA
CAAGATCTCAATGAAGGAAACCGCACTCTCACACTGGCCTTGATTTGGCAGCTAATGAGA
AGGTATACACTGAATATCCTCGAAGAAATTGGTGGTGGCCAGAAGGTCAATGATGACATT
ATTGTCAACTGGGTGAATGAAACATTGAGGGAAGCAGAGAAAAGTTCATCCATCTCTAGT
TTCAAGGACCCGAAGATTAGTACAAGTCTGCCTGTTCTGGACCTCATCGATGCCATCCAA
CCAGGTTCCATTAACTATGACCTTCTGAAGACAGAAAATCTGAATGATGATGAGAAACTC
AACAATGCAAAATATGCCATCTCTATGGCCCGAAAAATTGGAGCAAGAGTGTATGCCCTG
CCAGAAGACCTGGTTGAAGTGAACCCCAAAATGGTCATGACCGTGTTTGCCTGCCTCATG
GGGAAAGGAATGAAGAGGGTG

ORF Protein

Cloned ORF protein sequence for pFN21AE4543 Download

>KIEE4543  627 aa
MARGSVSDEEMMELREAFAKVDTDGNGYISFNELNDLFKAACLPLPGYRVREITENLMAT
GDLDQDGRISFDEFIKIFHGLKSTDVAKTFRKAINKKEGICAIGGTSEQSSVGTQHSYSE
EEKYAFVNWINKALENDPDCRHVIPMNPNTNDLFNAVGDGIVLCKMINLSVPDTIDERTI
NKKKLTPFTIQENLNLALNSASAIGCHVVNIGAEDLKEGKPYLVLGLLWQVIKIGLFADI
ELSRNEALIALLREGESLEDLMKLSPEELLLRWANYHLENAGCNKIGNFSTDIKDSKAYY
HLLEQVAPKGDEEGVPAVVIDMSGLREKDDIQRAECMLQQAERLGCRQFVTATDVVRGNP
KLNLAFIANLFNRYPALHKPENQDIDWGALEGETREERTFRNWMNSLGVNPRVNHLYSDL
SDALVIFQLYEKIKVPVDWNRVNKPPYPKLGGNMKKLENCNYAVELGKNQAKFSLVGIGG
QDLNEGNRTLTLALIWQLMRRYTLNILEEIGGGQKVNDDIIVNWVNETLREAEKSSSISS
FKDPKISTSLPVLDLIDAIQPGSINYDLLKTENLNDDEKLNNAKYAISMARKIGARVYAL
PEDLVEVNPKMVMTVFACLMGKGMKRV

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE4543 Download

>pFN21AE4543  6586 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCCAGAGGATCAGTGTCCGATGAGGAAATGAT
GGAGCTCAGAGAAGCTTTTGCCAAAGTTGATACTGATGGCAATGGATACATCAGCTTCAA
TGAGTTGAATGACTTGTTCAAGGCTGCTTGCTTGCCTTTGCCTGGGTATAGAGTACGAGA
AATTACAGAAAACCTGATGGCTACAGGTGATCTGGACCAAGATGGAAGGATCAGCTTTGA
TGAGTTTATCAAGATTTTCCATGGCCTAAAAAGCACAGATGTTGCCAAGACCTTTAGAAA
AGCAATCAATAAGAAGGAAGGGATTTGTGCAATCGGTGGTACTTCAGAGCAGTCTAGCGT
TGGCACCCAACACTCCTATTCAGAGGAAGAAAAGTATGCCTTTGTCAACTGGATAAACAA
AGCCCTGGAAAATGATCCTGATTGTCGGCATGTCATCCCAATGAACCCAAACACGAATGA
TCTCTTTAATGCTGTTGGAGATGGCATTGTCCTTTGTAAAATGATCAACCTGTCAGTGCC
AGACACAATTGATGAAAGAACAATCAACAAAAAGAAGCTAACCCCTTTCACCATTCAGGA
AAATCTGAACTTGGCTCTGAACTCTGCCTCAGCCATCGGGTGCCATGTGGTCAACATAGG
GGCTGAGGACCTGAAGGAGGGGAAGCCTTATCTGGTCCTGGGACTTCTGTGGCAAGTCAT
CAAGATTGGGTTGTTTGCTGACATTGAACTCAGCAGAAATGAAGCTCTGATTGCTCTTTT
GAGAGAAGGTGAGAGCCTGGAGGATTTGATGAAACTCTCCCCTGAAGAGCTCTTGCTGAG
GTGGGCTAATTACCACCTGGAAAATGCAGGCTGCAACAAAATTGGCAACTTCAGTACTGA
CATCAAGGACTCAAAAGCTTATTACCACCTGCTTGAGCAGGTGGCTCCAAAAGGAGATGA
AGAAGGTGTTCCTGCTGTTGTTATTGACATGTCAGGACTGCGGGAGAAGGATGACATCCA
GAGGGCAGAATGCATGCTGCAGCAGGCGGAGAGGCTGGGCTGCCGGCAGTTTGTCACAGC
CACAGATGTTGTCCGAGGGAACCCCAAGTTGAACTTGGCTTTTATTGCCAACCTCTTTAA
CAGATACCCTGCCCTGCACAAACCAGAGAACCAGGACATTGACTGGGGGGCTCTTGAAGG
TGAGACGAGAGAAGAGCGGACATTTAGGAACTGGATGAACTCCCTGGGTGTTAACCCTCG
AGTCAATCATTTGTACAGTGACTTATCAGATGCCCTGGTCATCTTCCAGCTCTATGAAAA
GATCAAAGTTCCTGTTGACTGGAACAGAGTAAACAAACCGCCATACCCCAAACTGGGAGG
CAATATGAAGAAGCTTGAGAATTGTAACTACGCGGTAGAATTGGGGAAGAATCAAGCGAA
GTTCTCCCTGGTTGGCATCGGTGGACAAGATCTCAATGAAGGAAACCGCACTCTCACACT
GGCCTTGATTTGGCAGCTAATGAGAAGGTATACACTGAATATCCTCGAAGAAATTGGTGG
TGGCCAGAAGGTCAATGATGACATTATTGTCAACTGGGTGAATGAAACATTGAGGGAAGC
AGAGAAAAGTTCATCCATCTCTAGTTTCAAGGACCCGAAGATTAGTACAAGTCTGCCTGT
TCTGGACCTCATCGATGCCATCCAACCAGGTTCCATTAACTATGACCTTCTGAAGACAGA
AAATCTGAATGATGATGAGAAACTCAACAATGCAAAATATGCCATCTCTATGGCCCGAAA
AATTGGAGCAAGAGTGTATGCCCTGCCAGAAGACCTGGTTGAAGTGAACCCCAAAATGGT
CATGACCGTGTTTGCCTGCCTCATGGGGAAAGGAATGAAGAGGGTGGTTTAAACGAATTC
GGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTG
CTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCAT
AACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAA
ACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCT
TTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTT
ATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAA
TGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATA
GCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTA
ATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGT
TTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATG
CTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATT
CCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTA
AAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGC
GGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAA
GTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGC
CGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTT
ACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACT
GCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCAC
AACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATA
CCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTA
TTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCG
GATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGAT
AAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT
AAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGA
AATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACC
GACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAG
AATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACC
GTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACA
AAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGT
TTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACC
TGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATC
TCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGC
CCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACT
TATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTG
CTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTA
TCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCA
AACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAA
AAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACG
AAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCC
TTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAAC
CATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTT
GCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGT
TTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGT
ACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAAC
CGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT