Restriction map for
pFN21AE4543
(Circular) (Six-base) MAPSORT of: pFN21AE4543.seq Check: 6738 from: 1 to: 6586
pFN21AE4543 6586 bp
With 182 enzymes: SgfI *
March 28, 2012 14:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2908 2916 2908
Size: 8 6578
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4396 278
Size: 53 83 186 936 2860 2468
Fragments arranged by size:
2860 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3509 3929 1937
Size: 1572 420 4594
Fragments arranged by size:
4594 1572 420
Acc65I G'GTAC_C
Cuts at: 3907 3907
Size: 6586
AclI AA'CG_TT
Cuts at: 1563 4713 5086 1563
Size: 3150 373 3063
Fragments arranged by size:
3150 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2307 2672 2823 2854 3731 4649 6033 6301
Size: 675 365 151 31 877 918 1384 268
Cuts at: 6301 1632
Size: 1917
Fragments arranged by size:
1917 1384 918 877 675 365 268 151
31
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6389
AflIII A'CryG_T
Cuts at: 1203 2967 5485 6462 1203
Size: 1764 2518 977 1327
Fragments arranged by size:
2518 1764 1327 977
AgeI A'CCGG_T
Cuts at: 5417 5417
Size: 6586
AhdI GACnn_n'nnGTC
Cuts at: 5315 5315
Size: 6586
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6586
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6586
AlwNI CAG_nnn'CTG
Cuts at: 4329 5901 4329
Size: 1572 5014
ApaLI G'TGCA_C
Cuts at: 4643 5799 4643
Size: 1156 5430
ApoI r'AATT_y
Cuts at: 1493 3895 4121 4273 6382 1493
Size: 2402 226 152 2109 1697
Fragments arranged by size:
2402 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5140 160
Size: 872 4108 1606
Fragments arranged by size:
4108 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6586
AvaI C'yCGr_G
Cuts at: 1870 1943 3237 3911 1870
Size: 73 1294 674 4545
Fragments arranged by size:
4545 1294 674 73
BamHI G'GATC_C
Cuts at: 3916 4283 3916
Size: 367 6219
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2342 2619 3907 5362 618
Size: 325 217 627 555 277 1288 1455 1842
Fragments arranged by size:
1842 1455 1288 627 555 325 277 217
BanII G_rGCy'C
Cuts at: 1391 2046 2811 3172 3905 1391
Size: 655 765 361 733 4072
Fragments arranged by size:
4072 765 733 655 361
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3719 3816 928
Size: 971 1820 97 3698
Fragments arranged by size:
3698 1820 971 97
BbvCI CC'TCA_GC
Cuts at: 2608 2643 2816 2608
Size: 35 173 6378
Fragments arranged by size:
6378 173 35
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4777 4811 1903
Size: 34 2840 34 3678
Fragments arranged by size:
3678 2840 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2078 4478 5688 2078
Size: 2400 1210 2976
Fragments arranged by size:
2976 2400 1210
BclI T'GATC_A
Cuts at: 1548 2502 1548
Size: 954 5632
BfrBI ATG'CAT
Cuts at: 3012 3012
Size: 6586
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5197 136
Size: 107 122 71 631 605 3525 1525
Fragments arranged by size:
3525 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3448 6581 3448
Size: 3133 3453
BlpI GC'TnA_GC
Cuts at: 4003 4003
Size: 6586
Bme1580I G_kGCm'C
Cuts at: 1790 4647 5803 1790
Size: 2857 1156 2573
Fragments arranged by size:
2857 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6586
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3172 3362 3583
Size: 416 53 83 399 24 1755 190 221
Cuts at: 3583 4317 5275 442
Size: 734 958 1753
Fragments arranged by size:
1755 1753 958 734 416 399 221 190
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6586
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2798 5246 1239
Size: 1559 2448 2579
Fragments arranged by size:
2579 2448 1559
Bpu10I CC'TnA_GC
Cuts at: 2608 2643 2816 2608
Size: 35 173 6378
Fragments arranged by size:
6378 173 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2933 3395 4711 5576 5874 2933
Size: 462 1316 865 298 3645
Fragments arranged by size:
3645 1316 865 462 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5249 882
Size: 617 3750 2219
Fragments arranged by size:
3750 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4377 493
Size: 711 3173 2702
Fragments arranged by size:
3173 2702 711
BsaBI GATnn'nnATC
Cuts at: 6580 6580
Size: 6586
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4393 4775 5410 275
Size: 53 83 186 936 2860 382 635 1451
Fragments arranged by size:
2860 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5019 5417 5691 5838 6249 5019
Size: 398 274 147 411 5356
Fragments arranged by size:
5356 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3777 3807 1178
Size: 30 165 30 2374 30 3957
Fragments arranged by size:
3957 2374 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6586
BseYI C'CCAG_C
Cuts at: 3034 5789 6285 3034
Size: 2755 496 3335
Fragments arranged by size:
3335 2755 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3119 1163
Size: 573 1383 4630
Fragments arranged by size:
4630 1383 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4036 4797 5825 1540
Size: 275 186 2035 761 1028 2301
Fragments arranged by size:
2301 2035 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2046 2811 4647 4732 5803 2046
Size: 765 1836 85 1071 2829
Fragments arranged by size:
2829 1836 1071 765 85
BsmI GAATG_Cn'
Cuts at: 3014 4097 4190 3014
Size: 1083 93 5410
Fragments arranged by size:
5410 1083 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3176 3176
Size: 6586
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2811 3172 3905 4647 4732 5803
Size: 399 256 765 361 733 742 85 1071
Cuts at: 5803 1391
Size: 2174
Fragments arranged by size:
2174 1071 765 742 733 399 361 256
85
BspEI T'CCGG_A
Cuts at: 6249 6249
Size: 6586
BspHI T'CATG_A
Cuts at: 3840 4475 6205 3840
Size: 635 1730 4221
Fragments arranged by size:
4221 1730 635
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2908 2916 3941 844
Size: 2064 8 1025 3489
Fragments arranged by size:
3489 2064 1025 8
BsrBI CCG'CTC
Cuts at: 1394 1823 3196 4473 1394
Size: 429 1373 1277 3507
Fragments arranged by size:
3507 1373 1277 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2086 5081 5255 65
Size: 1183 838 2995 174 1396
Fragments arranged by size:
2995 1396 1183 838 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3042 5230 5417 1700
Size: 110 1232 2188 187 2869
Fragments arranged by size:
2869 2188 1232 187 110
BsrGI T'GTAC_A
Cuts at: 96 3252 96
Size: 3156 3430
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6586
BssSI C'ACGA_G
Cuts at: 4646 5658 4646
Size: 1012 5574
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3758 794
Size: 2964 3622
BstBI TT'CG_AA
Cuts at: 5390 5390
Size: 6586
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6586
BstXI CCAn_nnnn'nTGG
Cuts at: 3769 3769
Size: 6586
BstYI r'GATC_y
Cuts at: 1972 3448 3916 4283 4668 4685 6137 6223 6235
Size: 1476 468 367 385 17 1452 86 12
Cuts at: 6235 6581 1972
Size: 346 1977
Fragments arranged by size:
1977 1476 1452 468 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3510 3510
Size: 6586
BtgI C'CryG_G
Cuts at: 513 1065 2004 2239 513
Size: 552 939 235 4860
Fragments arranged by size:
4860 939 552 235
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5847
Fragments arranged by size:
5847 683 56
BtsI GCAGTG_nn'
Cuts at: 4102 4896 4916 4102
Size: 794 20 5772
Fragments arranged by size:
5772 794 20
ClaI AT'CG_AT
Cuts at: 1554 3672 1554
Size: 2118 4468
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3049 3084 529
Size: 35 2485 35 4031
Fragments arranged by size:
4031 2485 35 35
DraI TTT'AAA
Cuts at: 3890 3890
Size: 6586
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3566 5593 809
Size: 507 2250 2027 1802
Fragments arranged by size:
2250 2027 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3541 4033
Size: 54 1425 50 275 18 177 1534 492
Cuts at: 4033 4922 6551 8
Size: 889 1629 43
Fragments arranged by size:
1629 1534 1425 889 492 275 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4033 1537
Size: 275 2221 4090
Fragments arranged by size:
4090 2221 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2799 3186 3872 4516 1353
Size: 1446 387 686 644 3423
Fragments arranged by size:
3423 1446 686 644 387
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3041 5173 5547 5693 387
Size: 1064 138 1452 2132 374 146 1280
Fragments arranged by size:
2132 1452 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2044 2809 2044
Size: 765 5821
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2307 2672 2798 2823 2854 3731 4649
Size: 393 675 365 126 25 31 877 918
Cuts at: 4649 5246 6033 6301 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 918 877 787 675 597 393 365
268 126 31 25
EcoNI CCTnn'n_nnAGG
Cuts at: 2654 2654
Size: 6586
EcoO109I rG'GnC_Cy
Cuts at: 2647 3631 2647
Size: 984 5602
EcoRI G'AATT_C
Cuts at: 3895 4273 3895
Size: 378 6208
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2395 3109 2395
Size: 714 5872
FspI TGC'GCA
Cuts at: 5092 5092
Size: 6586
HaeII r_GCGC'y
Cuts at: 1678 5733 1678
Size: 4055 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3435 3467 3706 3738 5227
Size: 32 135 32 1863 32 239 32 1489
Cuts at: 5227 5259 5301 5333 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1863 1489 239 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2386 2632 3232 3316 3571 3930
Size: 988 281 448 246 600 84 255 359
Cuts at: 3930 4176 669
Size: 246 3079
Fragments arranged by size:
3079 988 600 448 359 281 255 246
246 84
HindIII A'AGCT_T
Cuts at: 748 2052 2895 3371 748
Size: 1304 843 476 3963
Fragments arranged by size:
3963 1304 843 476
HpaI GTT'AAC
Cuts at: 3232 4176 3232
Size: 944 5642
KpnI G_GTAC'C
Cuts at: 3911 3911
Size: 6586
MfeI C'AATT_G
Cuts at: 2526 4185 2526
Size: 1659 4927
MluI A'CGCG_T
Cuts at: 6462 6462
Size: 6586
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2455 3706 5700 5884 1305
Size: 1150 1251 1994 184 2007
Fragments arranged by size:
2007 1994 1251 1150 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 3543 10
Size: 54 1425 343 177 1534 3053
Fragments arranged by size:
3053 1534 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2438 3767 3845 4544 4903 5062 518
Size: 648 1272 1329 78 699 359 159 2042
Fragments arranged by size:
2042 1329 1272 699 648 359 159 78
MspA1I CmG'CkG
Cuts at: 1606 4002 4679 5827 6072 1606
Size: 2396 677 1148 245 2120
Fragments arranged by size:
2396 2120 1148 677 245
NaeI GCC'GGC
Cuts at: 3044 3044
Size: 6586
NcoI C'CATG_G
Cuts at: 513 1065 2004 2239 513
Size: 552 939 235 4860
Fragments arranged by size:
4860 939 552 235
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6586
NgoMIV G'CCGG_C
Cuts at: 3042 3042
Size: 6586
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6586
NotI GC'GGCC_GC
Cuts at: 4033 4033
Size: 6586
NsiI A_TGCA'T
Cuts at: 3014 3014
Size: 6586
NspI r_CATG'y
Cuts at: 1132 1229 2432 2971 3016 3944 5489 1132
Size: 97 1203 539 45 928 1545 2229
Fragments arranged by size:
2229 1545 1203 928 539 97 45
PasI CC'CwG_GG
Cuts at: 1348 3223 1348
Size: 1875 4711
PciI A'CATG_T
Cuts at: 2967 5485 2967
Size: 2518 4068
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6586
PmeI GTTT'AAAC
Cuts at: 3890 3890
Size: 6586
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2591 2623 4655 4687 6168 6200 2591
Size: 32 2032 32 1481 32 2977
Fragments arranged by size:
2977 2032 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2647 3631 2647
Size: 984 5602
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6586
PsiI TTA'TAA
Cuts at: 4156 4156
Size: 6586
PspXI vC'TCGA_Gb
Cuts at: 3237 3237
Size: 6586
PstI C_TGCA'G
Cuts at: 830 3021 3938 830
Size: 2191 917 3478
Fragments arranged by size:
3478 2191 917
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6586
SacI G_AGCT'C
Cuts at: 2046 2811 2046
Size: 765 5821
SalI G'TCGA_C
Cuts at: 1936 3928 1936
Size: 1992 4594
SapI GCTCTTCn'nnn_
Cuts at: 2799 3186 2799
Size: 387 6199
SbfI CC_TGCA'GG
Cuts at: 3938 3938
Size: 6586
ScaI AGT'ACT
Cuts at: 2875 4834 6546 2875
Size: 1959 1712 2915
Fragments arranged by size:
2915 1959 1712
SexAI A'CCwGG_T
Cuts at: 3685 3813 3685
Size: 128 6458
SfcI C'TryA_G
Cuts at: 826 1045 2181 3017 3934 5069 5750 5941 826
Size: 219 1136 836 917 1135 681 191 1471
Fragments arranged by size:
1471 1136 1135 917 836 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6586
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6586
SmaI CCC'GGG
Cuts at: 1872 3913 1872
Size: 2041 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2912 3237 3374 4690 5591 5853
Size: 197 926 969 325 137 1316 901 262
Cuts at: 5853 820
Size: 1553
Fragments arranged by size:
1553 1316 969 926 901 325 262 197
137
SnaBI TAC'GTA
Cuts at: 493 4377 493
Size: 3884 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6586
SphI G_CATG'C
Cuts at: 1132 3016 3944 1132
Size: 1884 928 3774
Fragments arranged by size:
3774 1884 928
SspI AAT'ATT
Cuts at: 5 52 4510 5
Size: 47 4458 2081
Fragments arranged by size:
4458 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6586
StyI C'CwwG_G
Cuts at: 513 1065 2004 2239 4025 513
Size: 552 939 235 1786 3074
Fragments arranged by size:
3074 1786 939 552 235
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2360 3215 3565 4613 4630 4783 4968
Size: 1255 780 855 350 1048 17 153 185
Cuts at: 4968 5413 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1048 855 780 445 350 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2873 3252 3644
Size: 274 80 33 51 1443 896 379 392
Cuts at: 3644 4832 6544 96
Size: 1188 1712 138
Fragments arranged by size:
1712 1443 1188 896 392 379 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3504 3536 3777 3809 6546 6578 3504
Size: 32 241 32 2737 32 3512
Fragments arranged by size:
3512 2737 241 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6586
XbaI T'CTAG_A
Cuts at: 3922 3922
Size: 6586
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3091 3091
Size: 6586
XhoI C'TCGA_G
Cuts at: 1943 3237 1943
Size: 1294 5292
XmaI C'CCGG_G
Cuts at: 1870 3911 1870
Size: 2041 4545
XmnI GAAnn'nnTTC
Cuts at: 2946 4715 2946
Size: 1769 4817
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4394 276
Size: 53 83 186 936 2860 2468
Fragments arranged by size:
2860 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspXI PstI PvuI SacI SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BplI
BsiWI Bsu36I DraIII EcoRV FseI FspAI KasI NarI
NruI PacI PfoI PmlI PspOMI PsrI PvuII RsrII
SacII SanDI SfiI SfoI SrfI SwaI