KLHL32 ORF clone pFN21AE4168

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE4168

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Clone info.

Species

human

Clone Name

pFN21AE4168 Sequence (with vector) Restriction Map

Product ID

FHC11702

ORF Sequence

DNA sequence (1860 bp)
Protein sequence (620 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE4168

Symbol

KLHL32

Alias : BKLHD5, KIAA1900, UG0030H05, dJ21F7.1

Description

kelch-like family member 32, transcript variant 1

Original Clone ID

cp94168

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1860 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 620 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS5038.1 0 100.0 KLHL32 CCDS69154.1 0 94.2 KLHL32 CCDS69155.1 2.4e-211 88.9 KLHL32 CCDS10948.1 7.3e-70 35.8 KLHL36 CCDS75495.1 1.6e-65 100.0 KLHL32 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR013069 32 138 PF00651 BTB/POZ IPR011705 145 243 PF07707 BTB/Kelch-associated IPR006652 336 384 PF01344 Kelch repeat type 1 IPR006652 390 432 PF01344 Kelch repeat type 1 IPR006652 437 480 PF01344 Kelch repeat type 1 IPR006652 483 529 PF01344 Kelch repeat type 1 HMMSmart IPR000210 42 139 SM00225 BTB/POZ-like IPR011705 144 245 SM00875 BTB/Kelch-associated IPR006652 290 346 SM00612 Kelch repeat type 1 IPR006652 347 398 SM00612 Kelch repeat type 1 IPR006652 399 446 SM00612 Kelch repeat type 1 IPR006652 447 494 SM00612 Kelch repeat type 1 IPR006652 495 547 SM00612 Kelch repeat type 1 ProfileScan IPR000210 42 109 PS50097 BTB/POZ-like
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001003760 634 610749 31.0 93.2 XP_016866347 634 610749 31.0 93.2 XP_005249164 634 610749 31.0 93.2 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE4168 Download

>KIEE4168  1860 bp
ATGCCGTCTGAACGCTGCCTCAGTATTCAAGAAATGCTGACAGGCCAGAGGCTCTGCCAC
TCCGAATCTCACAATGACAGTGTCCTGGCAGCGCTGAATCAGCAGAGGAGTGATGGCATC
CTCTGCGACATCACCCTGATTGCTGAGGAACAGAAATTCCATGCTCACAAGGCAGTCCTA
GCAGCATGCAGTGACTATTTCCGGGCAATGTTCAGTCTTTGTATGGTGGAAAGTGGAGCT
GATGAGGTTAATTTGCACGGTGTGACCAGCCTTGGCTTAAAGCAGGCTCTGGAGTTTGCA
TACACAGGACAGATTTTGCTGGAGCCAGGTGTGATCCAGGATGTGCTAGCAGCGGGCAGT
CACCTACAGCTGTTGGAGCTTCTCAATTTATGCTCCCACTATCTCATCCAGGAATTAAAT
AGCTTTAATTACTTGGATCTGTACAGACTTGCTGACCTCTTTAACCTCACTTTGTTGGAG
AAGGCAGTGATCGATTTCTTAGTGAAACATCTCTCTGAACTCCTGAAGAGCCGCCCAGAA
GAAGTTCTAACGCTTCCCTATTGCCTGCTTCAGGAGGTGCTGAAGAGCGACCGCCTGACC
TCCCTGAGTGAAGAGCAGATCTGGCAGCTAGCTGTGAGGTGGTTGGAACACAACTGCCAC
TACCAGTACATGGACGAGCTCCTGCAATACATCCGCTTTGGCCTAATGGATGTGGATACT
CTCCATACAGTTGCCCTGTCCCACCCCCTTGTCCAAGCAAGTGAGACTGCAACAGCCCTT
GTCAACGAGGCCCTGGAATACCACCAGAGCATCTATGCACAGCCTGTCTGGCAGACTCGC
AGGACCAAACCACGATTCCAGTCAGACACTCTGTATATCATTGGTGGGAAAAAGCGCGAG
GTCTGCAAGGTCAAGGAACTTCGGTACTTCAATCCTGTTGATCAGGAGAATGCTCTCATA
GCTGCCATTGCCAACTGGAGTGAGCTGGCTCCCATGCCTGTGGGAAGGAGCCACCATTGT
GTGGCAGTCATGGGGGACTTCCTGTTTGTGGCAGGAGGGGAAGTTGAGCATGCCAGTGGC
CGGACGTGTGCTGTGAGGACTGCCTGTCGCTATGACCCCCGCAGTAATTCCTGGGCAGAG
ATAGCACCCATGAAAAACTGCCGGGAGCATTTTGTGCTGGGTGCCATGGAGGAATACCTC
TATGCAGTTGGGGGCAGAAATGAACTGCGCCAGGTTCTGCCTACAGTTGAGCGATATTGC
CCCAAGAAGAACAAATGGACTTTTGTTCAGTCCTTTGACAGATCCCTTTCATGCCATGCT
GGATATGTGGCTGATGGTCTTCTTTGGATATCAGGTGGAGTAACTAATACGGCACAATAT
CAGAACAGGCTAATGGTGTATGAACCTAACCAGAATAAGTGGATAAGCCGTAGCCCCATG
CTGCAGAGAAGGGTCTACCATTCCATGGCTGCTGTACAAAGGAAGCTTTATGTTCTTGGA
GGCAATGACCTAGACTACAATAATGACCGGATCCTTGTGCGCCATATAGATTCTTACAAC
ATAGACACTGACCAGTGGACACGTTGTAATTTCAACCTGCTGACTGGCCAAAATGAATCT
GGAGTTGCTGTCCATAATGGGAGAATATATTTAGTTGGTGGATATTCAATTTGGACAAAT
GAGCCTCTGGCTTGTATCCAGGTACTGGATGTAAGCAGAGAAGGCAAAGAAGAAGTATTC
TATGGGCCTACACTCCCTTTTGCTTCCAATGGAATAGCAGCATGCTTCCTTCCAGCTCCA
TATTTTACATGCCCTAACCTTCAAACTCTTCAAGTGCCTCATCACAGGATTGGCACCATC

ORF Protein

Cloned ORF protein sequence for pFN21AE4168 Download

>KIEE4168  620 aa
MPSERCLSIQEMLTGQRLCHSESHNDSVLAALNQQRSDGILCDITLIAEEQKFHAHKAVL
AACSDYFRAMFSLCMVESGADEVNLHGVTSLGLKQALEFAYTGQILLEPGVIQDVLAAGS
HLQLLELLNLCSHYLIQELNSFNYLDLYRLADLFNLTLLEKAVIDFLVKHLSELLKSRPE
EVLTLPYCLLQEVLKSDRLTSLSEEQIWQLAVRWLEHNCHYQYMDELLQYIRFGLMDVDT
LHTVALSHPLVQASETATALVNEALEYHQSIYAQPVWQTRRTKPRFQSDTLYIIGGKKRE
VCKVKELRYFNPVDQENALIAAIANWSELAPMPVGRSHHCVAVMGDFLFVAGGEVEHASG
RTCAVRTACRYDPRSNSWAEIAPMKNCREHFVLGAMEEYLYAVGGRNELRQVLPTVERYC
PKKNKWTFVQSFDRSLSCHAGYVADGLLWISGGVTNTAQYQNRLMVYEPNQNKWISRSPM
LQRRVYHSMAAVQRKLYVLGGNDLDYNNDRILVRHIDSYNIDTDQWTRCNFNLLTGQNES
GVAVHNGRIYLVGGYSIWTNEPLACIQVLDVSREGKEEVFYGPTLPFASNGIAACFLPAP
YFTCPNLQTLQVPHHRIGTI

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE4168 Download

>pFN21AE4168  6565 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCCGTCTGAACGCTGCCTCAGTATTCAAGAAAT
GCTGACAGGCCAGAGGCTCTGCCACTCCGAATCTCACAATGACAGTGTCCTGGCAGCGCT
GAATCAGCAGAGGAGTGATGGCATCCTCTGCGACATCACCCTGATTGCTGAGGAACAGAA
ATTCCATGCTCACAAGGCAGTCCTAGCAGCATGCAGTGACTATTTCCGGGCAATGTTCAG
TCTTTGTATGGTGGAAAGTGGAGCTGATGAGGTTAATTTGCACGGTGTGACCAGCCTTGG
CTTAAAGCAGGCTCTGGAGTTTGCATACACAGGACAGATTTTGCTGGAGCCAGGTGTGAT
CCAGGATGTGCTAGCAGCGGGCAGTCACCTACAGCTGTTGGAGCTTCTCAATTTATGCTC
CCACTATCTCATCCAGGAATTAAATAGCTTTAATTACTTGGATCTGTACAGACTTGCTGA
CCTCTTTAACCTCACTTTGTTGGAGAAGGCAGTGATCGATTTCTTAGTGAAACATCTCTC
TGAACTCCTGAAGAGCCGCCCAGAAGAAGTTCTAACGCTTCCCTATTGCCTGCTTCAGGA
GGTGCTGAAGAGCGACCGCCTGACCTCCCTGAGTGAAGAGCAGATCTGGCAGCTAGCTGT
GAGGTGGTTGGAACACAACTGCCACTACCAGTACATGGACGAGCTCCTGCAATACATCCG
CTTTGGCCTAATGGATGTGGATACTCTCCATACAGTTGCCCTGTCCCACCCCCTTGTCCA
AGCAAGTGAGACTGCAACAGCCCTTGTCAACGAGGCCCTGGAATACCACCAGAGCATCTA
TGCACAGCCTGTCTGGCAGACTCGCAGGACCAAACCACGATTCCAGTCAGACACTCTGTA
TATCATTGGTGGGAAAAAGCGCGAGGTCTGCAAGGTCAAGGAACTTCGGTACTTCAATCC
TGTTGATCAGGAGAATGCTCTCATAGCTGCCATTGCCAACTGGAGTGAGCTGGCTCCCAT
GCCTGTGGGAAGGAGCCACCATTGTGTGGCAGTCATGGGGGACTTCCTGTTTGTGGCAGG
AGGGGAAGTTGAGCATGCCAGTGGCCGGACGTGTGCTGTGAGGACTGCCTGTCGCTATGA
CCCCCGCAGTAATTCCTGGGCAGAGATAGCACCCATGAAAAACTGCCGGGAGCATTTTGT
GCTGGGTGCCATGGAGGAATACCTCTATGCAGTTGGGGGCAGAAATGAACTGCGCCAGGT
TCTGCCTACAGTTGAGCGATATTGCCCCAAGAAGAACAAATGGACTTTTGTTCAGTCCTT
TGACAGATCCCTTTCATGCCATGCTGGATATGTGGCTGATGGTCTTCTTTGGATATCAGG
TGGAGTAACTAATACGGCACAATATCAGAACAGGCTAATGGTGTATGAACCTAACCAGAA
TAAGTGGATAAGCCGTAGCCCCATGCTGCAGAGAAGGGTCTACCATTCCATGGCTGCTGT
ACAAAGGAAGCTTTATGTTCTTGGAGGCAATGACCTAGACTACAATAATGACCGGATCCT
TGTGCGCCATATAGATTCTTACAACATAGACACTGACCAGTGGACACGTTGTAATTTCAA
CCTGCTGACTGGCCAAAATGAATCTGGAGTTGCTGTCCATAATGGGAGAATATATTTAGT
TGGTGGATATTCAATTTGGACAAATGAGCCTCTGGCTTGTATCCAGGTACTGGATGTAAG
CAGAGAAGGCAAAGAAGAAGTATTCTATGGGCCTACACTCCCTTTTGCTTCCAATGGAAT
AGCAGCATGCTTCCTTCCAGCTCCATATTTTACATGCCCTAACCTTCAAACTCTTCAAGT
GCCTCATCACAGGATTGGCACCATCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCC
TCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAG
CTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTT
CGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGA
AAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGC
TGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAG
ATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAA
TGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATC
CGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGT
GGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCA
AATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGG
AAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC
CTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTG
GGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTT
CGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTA
TTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAAT
GACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGA
GAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACA
ACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACT
CGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACC
ACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACT
CTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTT
CTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGT
GGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTT
ATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATA
GGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGG
TATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAA
AGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGG
CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGA
GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG
TGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG
GAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTC
GCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCG
GTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCA
CTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGT
GGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAG
TTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCG
GTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATC
CTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTT
TGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAG
GAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAG
TGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTA
AAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTC
TGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAA
ATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATG
CGTCCGGCGTAGAGGATCGAGATCT