(Linear) (Six-base) MAPSORT of: KIEE4168.seq Check: 8361 from: 1 to: 1860 KIEE4168.orf With 182 enzymes: SgfI * July 3, 2012 18:42 .. AccI GT'mk_AC Cuts at: 0 1454 1860 Size: 1454 406 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 544 553 601 1860 Size: 544 9 48 1259 Fragments arranged by size: 1259 544 48 9 AfeI AGC'GCT Cuts at: 0 92 1860 Size: 92 1768 AflIII A'CryG_T Cuts at: 0 1084 1579 1860 Size: 1084 495 281 Fragments arranged by size: 1084 495 281 AhdI GACnn_n'nnGTC Cuts at: 0 1103 1860 Size: 1103 757 AleI CACnn'nnGTG Cuts at: 0 1016 1860 Size: 1016 844 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 140 172 1860 Size: 140 32 1688 Fragments arranged by size: 1688 140 32 AlwNI CAG_nnn'CTG Cuts at: 0 288 1236 1704 1860 Size: 288 948 468 156 Fragments arranged by size: 948 468 288 156 ApoI r'AATT_y Cuts at: 0 154 1860 Size: 154 1706 BamHI G'GATC_C Cuts at: 0 1529 1860 Size: 1529 331 BanI G'GyrC_C Cuts at: 0 1180 1852 1860 Size: 1180 672 8 Fragments arranged by size: 1180 672 8 BanII G_rGCy'C Cuts at: 0 680 1860 Size: 680 1180 BbsI GAAGACnn'nnnn_ Cuts at: 0 1330 1860 Size: 1330 530 BbvCI CC'TCA_GC Cuts at: 0 143 1860 Size: 143 1717 BciVI GTATCCnnnnn_n' Cuts at: 0 708 1705 1860 Size: 708 997 155 Fragments arranged by size: 997 708 155 BclI T'GATC_A Cuts at: 0 939 1860 Size: 939 921 BglI GCCn_nnn'nGGC Cuts at: 0 828 1860 Size: 828 1032 BglII A'GATC_T Cuts at: 0 617 1860 Size: 617 1243 BmgBI CAC'GTC Cuts at: 0 1085 1860 Size: 1085 775 BmtI G_CTAG'C Cuts at: 0 349 631 1860 Size: 349 282 1229 Fragments arranged by size: 1229 349 282 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 310 340 996 1640 1860 Size: 310 30 656 644 220 Fragments arranged by size: 656 644 310 220 30 Bpu10I CC'TnA_GC Cuts at: 0 143 1860 Size: 143 1717 BsaWI w'CCGG_w Cuts at: 0 1526 1860 Size: 1526 334 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1046 1076 1613 1643 1860 Size: 1046 30 537 30 217 Fragments arranged by size: 1046 537 217 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 120 1860 Size: 120 1740 BseYI C'CCAG_C Cuts at: 0 1176 1860 Size: 1176 684 BsiEI CG_ry'CG Cuts at: 0 591 1860 Size: 591 1269 BsiHKAI G_wGCw'C Cuts at: 0 680 1860 Size: 680 1180 BsmI GAATG_Cn' Cuts at: 0 954 1860 Size: 954 906 Bsp1286I G_dGCh'C Cuts at: 0 680 1860 Size: 680 1180 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1604 1860 Size: 1604 256 BsrDI GCAATG_nn' Cuts at: 0 212 965 1509 1860 Size: 212 753 544 351 Fragments arranged by size: 753 544 351 212 BsrGI T'GTAC_A Cuts at: 0 440 1473 1860 Size: 440 1033 387 Fragments arranged by size: 1033 440 387 BstAPI GCAn_nnn'nTGC Cuts at: 0 122 1173 1860 Size: 122 1051 687 Fragments arranged by size: 1051 687 122 BstXI CCAn_nnnn'nTGG Cuts at: 0 999 1860 Size: 999 861 BstYI r'GATC_y Cuts at: 0 435 617 1300 1529 1860 Size: 435 182 683 229 331 Fragments arranged by size: 683 435 331 229 182 BtgI C'CryG_G Cuts at: 0 1184 1463 1860 Size: 1184 279 397 Fragments arranged by size: 1184 397 279 BtsI GCAGTG_nn' Cuts at: 0 195 491 1860 Size: 195 296 1369 Fragments arranged by size: 1369 296 195 ClaI AT'CG_AT Cuts at: 0 491 1860 Size: 491 1369 DraIII CAC_nnn'GTG Cuts at: 0 261 1860 Size: 261 1599 EaeI y'GGCC_r Cuts at: 0 1077 1605 1860 Size: 1077 528 255 Fragments arranged by size: 1077 528 255 EarI CTCTTCn'nnn_ Cuts at: 0 520 577 605 1832 1860 Size: 520 57 28 1227 28 Fragments arranged by size: 1227 520 57 28 28 EcoICRI GAG'CTC Cuts at: 0 678 1860 Size: 678 1182 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 310 340 544 553 601 996 1640 1860 Size: 310 30 204 9 48 395 644 220 Fragments arranged by size: 644 395 310 220 204 48 30 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 568 1860 Size: 568 1292 EcoO109I rG'GnC_Cy Cuts at: 0 789 1860 Size: 789 1071 EcoRV GAT'ATC Cuts at: 0 1349 1860 Size: 1349 511 HaeII r_GCGC'y Cuts at: 0 94 1860 Size: 94 1766 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1089 1121 1613 1645 1860 Size: 1089 32 492 32 215 Fragments arranged by size: 1089 492 215 32 32 HincII GTy'rAC Cuts at: 0 783 1860 Size: 783 1077 HindIII A'AGCT_T Cuts at: 0 1483 1860 Size: 1483 377 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 353 455 623 1860 Size: 353 102 168 1237 Fragments arranged by size: 1237 353 168 102 MscI TGG'CCA Cuts at: 0 1607 1860 Size: 1607 253 MslI CAynn'nnrTG Cuts at: 0 997 1016 1073 1860 Size: 997 19 57 787 Fragments arranged by size: 997 787 57 19 MspA1I CmG'CkG Cuts at: 0 352 369 1860 Size: 352 17 1491 Fragments arranged by size: 1491 352 17 NcoI C'CATG_G Cuts at: 0 1184 1463 1860 Size: 1184 279 397 Fragments arranged by size: 1184 397 279 NheI G'CTAG_C Cuts at: 0 345 627 1860 Size: 345 282 1233 Fragments arranged by size: 1233 345 282 NspI r_CATG'y Cuts at: 0 188 1072 1784 1811 1860 Size: 188 884 712 27 49 Fragments arranged by size: 884 712 188 49 27 PflMI CCAn_nnn'nTGG Cuts at: 0 669 1020 1464 1704 1860 Size: 669 351 444 240 156 Fragments arranged by size: 669 444 351 240 156 PfoI T'CCnGG_A Cuts at: 0 335 407 1860 Size: 335 72 1453 Fragments arranged by size: 1453 335 72 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2 34 1860 Size: 2 32 1826 Fragments arranged by size: 1826 32 2 PstI C_TGCA'G Cuts at: 0 1445 1860 Size: 1445 415 PvuII CAG'CTG Cuts at: 0 369 1860 Size: 369 1491 SacI G_AGCT'C Cuts at: 0 680 1860 Size: 680 1180 SapI GCTCTTCn'nnn_ Cuts at: 0 520 577 605 1860 Size: 520 57 28 1255 Fragments arranged by size: 1255 520 57 28 SfcI C'TryA_G Cuts at: 0 364 1241 1441 1860 Size: 364 877 200 419 Fragments arranged by size: 877 419 364 200 SphI G_CATG'C Cuts at: 0 188 1072 1784 1860 Size: 188 884 712 76 Fragments arranged by size: 884 712 188 76 StyI C'CwwG_G Cuts at: 0 270 1184 1463 1860 Size: 270 914 279 397 Fragments arranged by size: 914 397 279 270 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1161 1168 1860 Size: 1161 7 692 Fragments arranged by size: 1161 692 7 TatI w'GTAC_w Cuts at: 0 440 665 1473 1860 Size: 440 225 808 387 Fragments arranged by size: 808 440 387 225 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1338 1370 1860 Size: 1338 32 490 Fragments arranged by size: 1338 490 32 Tth111I GACn'n_nGTC Cuts at: 0 79 1860 Size: 79 1781 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1615 1860 Size: 1615 245 XmnI GAAnn'nnTTC Cuts at: 0 542 1860 Size: 542 1318 Enzymes that do cut: AccI AcuI AfeI AflIII AhdI AleI AloI AlwNI ApoI BamHI BanI BanII BbsI BbvCI BciVI BclI BglI BglII BmgBI BmtI BpmI Bpu10I BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrGI BstAPI BstXI BstYI BtgI BtsI ClaI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I HincII HindIII MmeI MscI MslI MspA1I NcoI NheI NspI PflMI PfoI PpiI PstI PvuII SacI SapI SfcI SphI StyI TaqII TatI TstI Tth111I XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AgeI AlfI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BbeI BcgI BfrBI BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsgI BsiWI BsmBI BspEI BspHI BsrBI BsrFI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI CspCI DraI DrdI EagI EciI EcoRI FalI FseI FspI FspAI HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NotI NruI NsiI PacI PasI PciI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI StuI SwaI XbaI XhoI XmaI ZraI