################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI Alw44I AocI ApaI ApaLI AscI AseI AsnI Asp718I AvaI AviII AvrII BbeI BbrPI BcgI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BseAI BsgI BsiWI BsmBI BsoBI BspEI BspHI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I CpoI DraI DrdI EagI EclXI Eco52I Eco81I EcoO65I EcoRI EcoT22I FseI FspI HgaI Hin1I HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspI StuI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AccI 1453 GTCTAC GT^[AC][GT]AC AccII 895 CGCG CG^CG AcsI 154 AAATTC [GA]^AATT[CT] AhdI 1098 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 90 AGCGCT AGC^GCT ApoI 154 AAATTC [GA]^AATT[CT] Asp700I 538 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1098 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 676 GAGCTC G[AT]GC[AT]^C AspI 76 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 842 GGACC G^G[AT]CC BalI 1605 TGGCCA TGG^CCA BamHI 1529 GGATCC G^GATCC BanII 676 GAGCTC G[GA]GC[CT]^C BbsI 1337 GTCTTC GAAGAC(2/6) BbvCI 142 GCTGAGG CCTCAGC(-5/-2) BclI 939 TGATCA T^GATCA BglI 822 GCCTGTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 617 AGATCT A^GATCT BmyI 676 GAGCTC G[AGT]GC[ACT]^C BpuAI 1337 GTCTTC GAAGAC(2/6) BsaWI 1526 ACCGGA [AT]^CCGG[AT] BseRI 105 GAGGAG GAGGAG(10/8) BsiEI 588 CGACCG CG[GA][CT]^CG BsiHKAI 676 GAGCTC G[AT]GC[AT]^C BsmAI 763 GAGAC GTCTC(1/5) BsmI 948 GAATGC GAATGC(1/-1) Bsp1286I 676 GAGCTC G[AGT]GC[ACT]^C BspDI 490 ATCGAT AT^CGAT BspMI 1595 ACCTGC ACCTGC(4/8) BstUI 895 CGCG CG^CG BstXI 992 CCATGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 490 ATCGAT AT^CGAT DraII 788 AGGCCCT [GA]G^G[ACGT]CC[CT] DraIII 256 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 1098 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 90 AGCGCT AGC^GCT EcoNI 564 CCTGCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 788 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoRV 1347 GATATC GAT^ATC FbaI 939 TGATCA T^GATCA HaeII 90 AGCGCT [GA]GCGC^[CT] HincII 781 GTCAAC GT[CT]^[GA]AC HindII 781 GTCAAC GT[CT]^[GA]AC HindIII 1483 AAGCTT A^AGCTT MluNI 1605 TGGCCA TGG^CCA MscI 1605 TGGCCA TGG^CCA MvnI 895 CGCG CG^CG PflFI 76 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 834 GACTC GAGTC(4/5) PstI 1441 CTGCAG CTGCA^G PvuII 367 CAGCTG CAG^CTG SacI 676 GAGCTC GAGCT^C TaqI 491 TCGA T^CGA Tth111I 76 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 491 TCGA T^CGA XcmI 1608 CCAAAATGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 538 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AflIII 1084 ACGTGT A^C[GA][CT]GT 1579 ACACGT BanI 1180 GGTGCC G^G[CT][GA]CC 1852 GGCACC BciVI 714 GGATAC GTATCC(6/5) 1694 GTATCC BcnI 201 CCGGG CC^[GC]GG 1161 CCGGG BsmFI 1034 GGGAC GGGAC(10/14) 738 GTCCC Bsp1407I 440 TGTACA T^GTACA 1473 TGTACA BsrGI 440 TGTACA T^GTACA 1473 TGTACA BstAPI 116 GCATCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1167 GCATTTTGTGC DsaI 1184 CCATGG C^C[GA][CT]GG 1463 CCATGG EaeI 1077 TGGCCG [CT]^GGCC[GA] 1605 TGGCCA FauI 1118 CCCGC CCCGC(4/6) 352 GCGGG HphI 131 TCACC GGTGA(8/7) 360 TCACC MaeII 1084 ACGT A^CGT 1581 ACGT MspA1I 350 CAGCGG C[AC]G^C[GT]G 367 CAGCTG NciI 201 CCGGG CC^[GC]GG 1161 CCGGG NcoI 1184 CCATGG C^CATGG 1463 CCATGG NheI 345 GCTAGC G^CTAGC 627 GCTAGC SfaNI 116 GCATC GCATC(5/9) 809 GCATC SspBI 440 TGTACA T^GTACA 1473 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI BbvI BfaI BpmI BsaJI BsiYI BslI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NdeII NlaIII NlaIV NspI PflMI RsaI SapI Sau3AI Sau96I ScrFI SfcI SphI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XhoII